Preparing method of mother culture special for production of Cordyceps militaris

A technology for Cordyceps militaris and parent species, applied in the directions of botanical equipment and methods, application, horticulture, etc., can solve the problems of unfavorable industrial production of Cordyceps militaris mycelium and fruiting bodies, long production cycle, high production cost, and reduce the risk of degradation. , The effect of inoculation is simple and the production cycle is saved

Inactive Publication Date: 2015-09-23
HUZHOU TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacteria need to be expanded and cultivated step by step. This production method has disadvantages such as long production cycle, easy pollution, high production cost, and easy degradation of bacteria.
These problems are not conducive to the industrial production of Cordyceps militaris mycelium and fruiting body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of special mother seed

[0032] Preparation method of special mother seed:

[0033] 1. Primary shake flask strain

[0034] ① Medium formula (g / L): 200g potato, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, 1g ammonium citrate, 30g glucose, 3g peptone, 50mg vitamin B1.

[0035] ②Production process:

[0036] Potato dip preparation: peel high-quality aseptic potatoes, wash them with water, weigh 200g potatoes, cut into 3-4mm slices, put them in a pot, add 1 liter of water and cook until crisp and not rotten, filter the residue with gauze, Take out the filtrate and pour it into a container to cool it for later use.

[0037] Ingredients: Weigh 2 g of potassium dihydrogen phosphate, 1 g of magnesium sulfate, 1 g of ammonium citrate, 30 g of glucose, 3 g of peptone, and 50 mg of vitamin B1, add the potato boiling liquid, stir evenly, and dilute to 1000 mL.

[0038] Dispense: Dispense the configured medium into 500mL conical flasks, 200mL per bottle. Put...

Embodiment 2

[0051] Example 2 Mass cultivation of mycelium

[0052] Fermentation medium formula: 20g peptone, 20g sucrose, MgSO4·7H 2 O 0.5g, KH 2 PO 4 1g, vegetable oil 20mL or (foaming enemy 0.3mL), water 1000mL, pH6.5.

[0053] Medium preparation: Weigh the above reagents according to the formula ratio to prepare 75L liquid medium.

[0054] Feeding: First calibrate the pH electrode and dissolved oxygen electrode of the fermenter, and then add the fermentation medium and defoamer vegetable oil or foam enemy.

[0055] Sterilization: Sterilize at 121-126°C for 30 minutes, wait until the temperature drops below 30°C for use. Adjust the speed of 150r / min, the ventilation rate of 1.5vvm, and adjust the dissolved oxygen concentration to 100%.

[0056] Seed liquid preparation: Add 300 mL of sterile water to the special mother eggplant bottle, mash the strain to make a bacterial suspension.

[0057] Inoculation: Aseptically inoculate the seed solution with the fire ring method.

[0058] Cultivation: Durin...

Embodiment 3

[0061] Example 3: Fruit body cultivation

[0062] Solid cultivation material formula: rice (30g) + nutrient solution 35mL.

[0063] Nutrition solution formula: (20g sucrose, 1g potassium dihydrogen phosphate, 5g magnesium sulfate, 1000mL water).

[0064] Medium preparation: weigh rice and prepare nutrient solution according to the formula.

[0065] Bottling: 14×8×12cm high plastic container, add 30g rice, then add 35mL nutrient solution, shake well, wrap and seal with high-pressure polypropylene film and rubber band.

[0066] Sterilization: autoclave at 121°C, sterilize for 30 minutes, take out the bottle after sterilization for cooling and inoculation.

[0067] Liquid strain preparation: Add 300mL sterile water into the special mother eggplant bottle, mash the strain to make a bacterial suspension.

[0068] Inoculation: Use a sterilized pipette tip (the mouth of the pipette tip is cut large with scissors) to suck the liquid bacteria, and each box is connected to 10mL of the liquid bacter...

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PUM

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Abstract

The invention relates to a preparing method of a mother culture special for production of Cordyceps militaris. The preparing method includes: adding, according to a certain ratio, nutrient solution containing cane sugar, monopotassium phosphate and magnesium sulfate to wheat bran and millet, adjusting pH to obtain a medium, charging the medium in a flask, performing sterilizing at 121 DEG C for 30 min, in aseptic conditions, inoculating a spawn of Cordyceps militaris, performing culturing constantly at 18 DEG C to 25 DEG C for 7 to 14 days to obtain the mother culture. After the mother culture is added a certain amount of aseptic water, the mother culture is inoculated to a fermentation tank or solid medium, and the mycelia and fruiting bodies of Cordyceps militaris can be produced on a large scale. The preparing method has the advantages that the step amplified cultivation process is omitted, the production cycle is shortened by greater than one third, the cost of seed step amplification equipment is reduced, the production cost is decreased by greater than one third, inoculating is simple, less contamination occurs, the risk of strain degeneration is reduced, and the mycelia and fruiting bodies of Cordyceps militaris can be industrially produced.

Description

Technical field: [0001] The invention relates to a preparation method of a special mother seed for the production of Cordyceps militaris, and belongs to the technical field of edible fungus production. Background technique: [0002] Cordyceps militaris L.Link, also known as Cordyceps militaris, Cordyceps militaris. Cordyceps militaris is one of the few medicinal fungi that can be cultivated manually on artificial media, silkworm or tussah pupa. The mycelium and fruiting bodies of Cordyceps militaris contain cordycepin, cordycepic acid, cordyceps polysaccharide, SOD, adenosine, guanosine, uridine and other medicinal ingredients, and are rich in a variety of essential amino acids and trace elements. Industrialization of Cordyceps militaris to obtain mycelium and fruiting bodies requires a large amount of liquid strains. The preparation process of the liquid strain of Cordyceps militaris includes: preparation of slant mother seed → first-level shake flask → second shake flask (exp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 刘洋
Owner HUZHOU TEACHERS COLLEGE
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