Preparation composition containing broccoli extract with effects of improving immunity and suppressing tumors and preparation method of preparation composition

A composition and anti-tumor technology, applied in the directions of anti-tumor drugs, drug combinations, medical preparations containing active ingredients, etc., can solve problems such as the preparation process of granules, tablets, and capsules, and reports on related patents that have not been seen.

Inactive Publication Date: 2015-09-23
北京高科联合生物工程技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the research reports on the improvement of immunity and anti-tumor aspects of the combination of these extracts, and there are no reports on the preparation process of such extracts to prepare granules, tablets, and capsules, and related patents

Method used

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  • Preparation composition containing broccoli extract with effects of improving immunity and suppressing tumors and preparation method of preparation composition

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the preparation of broccoli extract

[0014] Get 3kg broccoli seeds and pulverize through pulverizer, carry out mixed degreasing with 3kg sherwood oil, then isolate sherwood oil, add 15kg water and mix homogeneously, add acetic acid in the mixing solution, pH is adjusted to 6, add 300 gram mixed enzymes (papain : cellulase=1: 1), add deionized water 10kg, add appropriate amount of vitamin C, keep pH to be 6, stir at room temperature (25 ℃) and carry out hydrolysis reaction, make the glucosinolate in broccoli seed convert into radish Sulfur, hydrolysis reaction 8h. The solid part was separated by centrifugation, and the hydrolyzate was added to a vacuum concentration tank, and the extract was concentrated to half of the original volume at a vacuum degree of 0.06Mpa and 30°C. The concentrated solution was added to a chromatographic column with a column volume of 2 L of Hp850 resin, and the flow rate of the sample was 20 ml / min. After the resin was adsorbed,...

Embodiment 2

[0015] Embodiment 2: the preparation of composition particle

[0016] Take 1L of broccoli extract in Example 1, mix with 0.5kg chitosan oligosaccharide, 1kg ganoderma lucidum polysaccharide, 1kg cordyceps polysaccharide and 1kg dextrin, add deionized water, adjust to a slurry with a solid content of 10%, and spray dry Make powder, the air inlet temperature is 180°C, and the air outlet temperature is 80°C to obtain the composition powder. Add 20% starch to the powder of the composition, adopt fluidized bed granulation method to make granules, and dry to obtain granules.

Embodiment 3

[0017] Embodiment 3: the immune function test of composition

[0018] Experimental method: the experimental animals are selected clean grade female mice (body weight 20g ± 2g), divided into four groups, i.e. blank control group, low dose group, middle dose group and high dose group, the broccoli composition fed to the mice is The prepared composition granule of embodiment 2 is dissolved in the solution that distilled water gains, low, medium, high dose group is calculated by 5 times, 10 times, 30 times of people's recommended dose (people's recommended dose is 2-4g / d), respectively 300mg / kg for the low dose group, 600mg / kg for the middle dose group, and 1800mg / kg for the high dose group. The control group was given the same amount of distilled water. With reference to the methods in the "Technical Specifications for Health Food Inspection and Evaluation" issued by the Ministry of Health and Xue Bin's "Immunotoxicology Experimental Technology" (Beijing Medical University, Chin...

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Abstract

The invention provides an anti-tumor and immune-modulating composition, a preparation method and an application of the composition in preparing anti-tumor and immune-modulating medicines. The preparation composition is formed by mixing 1 to 20 parts of broccoli extracting solution, 0.5 to 1 part of chitosan oligosaccharide, 1 to 2 parts of ganoderan, 1 to 2 parts of cordyceps polysaccharide and 1 to 2 parts of dextrin in parts by weight; the preparation composition is characterized in that the content of sulforaphane is 2 to 15 mg/g and the content of polysaccharide is 200 to 400 mg/g in the preparation composition; the preparation method comprises the following steps: crushing, porphyrizing, filtering, hydrolyzing and macroporous resin adsorption separating broccoli seeds or corms to obtain a water solution containing sulforaphane, uniformly mixing the broccoli extracting solution, chitosan oligosaccharide, ganoderan, cordyceps polysaccharide and dextrin with water, spray drying into fine powder, preparing into granules, tablets, capsules and the like. Animals researches prove that the compound polysaccharide preparation has remarkable effects on improving immunity and suppressing tumors.

Description

technical field [0001] The invention belongs to the field of functional food and medicine, and relates to the preparation of broccoli extract and the composition formula and preparation method of the extract, chitosan oligosaccharide, ganoderma lucidum polysaccharide and cordyceps polysaccharide. Background technique [0002] In recent years, the incidence of cancer in my country is on the rise, and the research on various anti-cancer and anti-cancer health foods has become a research hotspot. Broccoli is rich in sulforaphane (1-isothiocyanate-4-methylsulfonylbutyl alkane), sulforaphane is the active ingredient with the strongest and best effect among all natural anti-cancer substances. The type II detoxification enzymes in the body can inhibit the production of type I enzymes at the same time, and finally discharge harmful components such as carcinogens and free radicals through a variety of enzyme systems. At the same time, this component will not remain in the human body an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/31A61K9/16A61K9/48A61K9/28A61P35/00A61P37/04A61K31/702A61K31/715
Inventor 林强
Owner 北京高科联合生物工程技术研究院有限公司
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