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Preparation method of pleurotus ostreatus fermentation broth and application of fermentation broth in degradation of aflatoxin B1

A technology of Pleurotus pilosula and fermentation liquid, which is applied in the field of microbial fermentation, can solve the problems of fewer bacteria reports and more fungi, and achieve the effects of fast degradation speed, mild conditions and simple process

Inactive Publication Date: 2015-10-21
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Bacteria and fungi are mainly used to degrade aflatoxin by microbial fermentation preparations. A large number of foreign data studies have shown that there are many fungi but few reports on bacteria.

Method used

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  • Preparation method of pleurotus ostreatus fermentation broth and application of fermentation broth in degradation of aflatoxin B1
  • Preparation method of pleurotus ostreatus fermentation broth and application of fermentation broth in degradation of aflatoxin B1
  • Preparation method of pleurotus ostreatus fermentation broth and application of fermentation broth in degradation of aflatoxin B1

Examples

Experimental program
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Effect test

Embodiment 1

[0041] The preparation method of Pleurotus pleurotus fermented liquid comprises inoculating Pleurotus otreatus CICC14012 in a sterile medium containing carbon source, nitrogen source, inorganic salts and trace elements for aerobic fermentation to prepare a fermented liquid;

[0042] Fermentation medium is made up of the raw material of following mass percentage:

[0043] Potato starch 6.5%, sucrose 2.0%, yeast extract 0.18%, ammonium sulfate 0.31%, potassium dihydrogen phosphate 0.28%, magnesium sulfate 0.17%, vitamin B 1 0.0001%, trace element solution 6.0%, veratrol 450 μL / L, Tween 80 0.13%, the rest is sterile water, pH=5.0;

[0044] Trace element solution: calcium chloride 1500mg, ferrous sulfate 150mg, zinc sulfate 100mg, copper sulfate 150mg, manganese sulfate 1000mg, cobalt sulfate 200mg, aluminum potassium sulfate 20mg, boric acid 10mg, sodium molybdate 20mg, dilute to 1000mL.

[0045] The technological conditions of aerated fermentation are as follows:

[0046] The ...

Embodiment 2

[0060] The difference between this embodiment and embodiment 1 is:

[0061] The fermentation medium is composed of the following raw materials in mass percentage:

[0062] Potato starch 7.0%, sucrose 1.8%, yeast extract 0.20%, ammonium sulfate 0.29%, potassium dihydrogen phosphate 0.25%, magnesium sulfate 0.18%, vitamin B 1 0.0002%, trace element solution 5.0%, veratrol 430μL / L, Tween 80 0.10%, the balance is sterile water, pH=4.9.

[0063] During the aerated fermentation process, the inoculum size is volume ratio = 6:100, the temperature is 28°C, the ventilation rate is 1:0.7vvm, the pressure is 0.04Mpa, and the culture period is not less than 265 hours.

[0064] The culture temperature in the preparation of the slant strain and the dish strain was 25° C., and the culture period was 5 days.

[0065] The preparation of the primary seed comprises the following process steps:

[0066] Inoculate the slant strains on the solid medium in the plate, culture at 25°C for 5 days, se...

Embodiment 3

[0072] The difference between this embodiment and embodiment 1 is:

[0073] The fermentation medium is composed of the following raw materials in mass percentage:

[0074] Potato starch 6.0%, sucrose 2.2%, yeast extract 0.22%, ammonium sulfate 0.33%, potassium dihydrogen phosphate 0.30%, magnesium sulfate 0.15%, vitamin B 1 0.0003%, trace element solution 5.5%, veratrol 470μL / L, Tween 80 0.12%, the balance is sterile water, pH=5.3.

[0075] During the aerated fermentation process, the inoculum size is volume ratio = 8:100, the temperature is 27°C, the ventilation rate is 1:1.2vvm, the pressure is 0.05Mpa, and the culture period is not less than 280 hours.

[0076] The culture temperature in the preparation of the slant strains and plate strains was 27° C., and the culture period was 4 days.

[0077] The preparation of the primary seed comprises the following process steps:

[0078] Inoculate the slant strains on the solid medium in the plate, culture at 27°C for 4 days, sel...

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Abstract

The invention relates to microbial fermentation, in particular to a preparation method of pleurotus ostreatus fermentation broth and application of the fermentation broth in degradation of aflatoxin B1. Pleurotus ostreatus CICC14012 is inoculated in an axenic culture medium containing carbon sources, nitrogen sources, inorganic salt and trace elements to conduct aerated fermentation cultivation to prepare the fermentation broth, and the fermentation broth can be used for degrading the virulence of the aflatoxin B1. By means of the method, the technical problems that an existing aflatoxin B1 removal method needs special conditions and special materials, is high in labor intensity and not complete in detoxification, has an effect on breaking nutritious components in processed fodder, and is severe in required condition, prone to producing chemical residuals and causing secondary pollution and the like are solved. The pleurotus ostreatus fermentation broth prepared through the method has the advantages of being easy and convenient in process, free of environment pollution and high in product safety, and has the advantages of being high in degradation speed, high in degradation rate, small in addition amount and the like when used for degrading the aflatoxin B1.

Description

technical field [0001] The invention relates to microbial fermentation, in particular to a method for preparing a fermented liquid of Pleurotus officinalis and the ability of the fermented liquid to degrade aflatoxin B 1 in the application. Background technique [0002] Pleurotus officinale, commonly known as Pleurotus ostreatus, is a fungus that forms fruiting bodies. Its growth environment is divided into two types: wild and artificial cultivation. The factor entity is rich in nutrients and can be eaten, also known as edible fungi. According to a large number of measurement data, every 100 grams of dried mushrooms contains about 20 grams of crude protein, 4 grams of crude fat, 50 grams of carbohydrates, and 6 grams of crude fiber. It also contains minerals, multivitamins and various trace elements. Its protein content is 2.6 times that of eggs and 4 times that of pork; the carbohydrate content accounts for 65.61% of the total, of which reducing sugar accounts for 54.73%;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A23L1/015C12R1/645
Inventor 胡常英王云鹏罗同阳郑翔高庆华刘春卯李领颇吴芳彤
Owner 河北省微生物研究所有限公司
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