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Cu2+-EDTA-Fe3O4 magnetic grain, preparation method and application

A technology of -edta-fe3o4 and edta-fe3o4, applied in the field of superparamagnetic functional materials, can solve the problem that chromatography is not suitable for direct processing of biological samples, and achieve the effects of excellent specificity, uniform particle size distribution, and mild preparation conditions

Active Publication Date: 2015-11-11
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional chromatography is not suitable for direct processing of biological samples containing suspended particles and fouling components

Method used

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  • Cu2+-EDTA-Fe3O4 magnetic grain, preparation method and application
  • Cu2+-EDTA-Fe3O4 magnetic grain, preparation method and application
  • Cu2+-EDTA-Fe3O4 magnetic grain, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Cu 2+ -EDTA-Fe 3 o 4 Preparation of magnetic particles

[0039] (1) Preparation of EDTA-Fe by hydrothermal method 3 o 4 Magnetic particles, accurately weigh 0.5g FeCl 3 ·6H 2 O (1.85mmol), add 15mL ethylene glycol and 15mL diethylene glycol to assist in ultrasonic dissolution, then add 1.8g urea (30mmol) and 0.35g disodium edetate (1.04mmol) while stirring, and stir at 70°C for 30min A uniform solution was formed, and the solution was poured into a 50 mL Teflon autoclave, placed in an oven, and then heated to 200°C for 10 hours of reaction. After the reaction, cool to room temperature, separate the product under an external magnetic field and wash thoroughly with ethanol and deionized water for 3 times, and finally dry to obtain the product EDTA-Fe 3 o 4 magnetic particles.

[0040] (2) EDTA-Fe 3 o 4 The magnetic particles were added to 0.2mmol / L copper sulfate solution, ultrasonicated for 5min at room temperature, the product was separated under a...

Embodiment 2

[0044] Example 2: Cu 2+ -EDTA-Fe 3 o 4 Preparation of magnetic particles

[0045] (1) Preparation of EDTA-Fe by hydrothermal method 3 o 4 Magnetic particles, accurately weigh 0.39gFeCl 3 ·6H 2 O (1.44mmol), add 13.4mL ethylene glycol and 16.6mL diethylene glycol to assist in ultrasonic dissolution, then add 1.44g urea (24mmol) and 0.28g disodium edetate (0.83mmol) while stirring, at 50°C Stir for 30 minutes to form a homogeneous solution, pour the solution into a 50 mL Teflon autoclave, put it in an oven, then raise the temperature to 190°C, and react for 12 hours. After the reaction, cool to room temperature, separate the product under an external magnetic field and wash thoroughly with ethanol and deionized water for 3 times, and finally dry to obtain the product EDTA-Fe 3 o 4 magnetic particles.

[0046] (2) EDTA-Fe 3 o 4 Add the magnetic particles into 0.1mmol / L copper chloride solution, ultrasonicate for 3min at room temperature, separate the product under an e...

Embodiment 3

[0047] Example 3: Cu 2+ -EDTA-Fe 3 o 4 Preparation of magnetic particles

[0048] (1) Preparation of EDTA-Fe by hydrothermal method 3 o 4 Magnetic particles, accurately weigh 0.61gFeCl 3 ·6H 2 O (2.26mmol), add 16.4mL of ethylene glycol and 13.6mL of diethylene glycol to assist in ultrasonic dissolution, then add 2.16g of urea (36mmol) and 0.42g of disodium edetate (1.24mmol) while stirring, at 60°C Stir for 30 minutes to form a homogeneous solution, pour the solution into a 50 mL Teflon autoclave, put it in an oven, then raise the temperature to 210° C., and react for 8 hours. After the reaction, cool to room temperature, separate the product under an external magnetic field and wash thoroughly with ethanol and deionized water for 3 times, and finally dry to obtain the product EDTA-Fe 3 o 4 magnetic particles.

[0049] (2) EDTA-Fe 3 o 4 Add the magnetic particles into 0.3mmol / L copper nitrate solution, ultrasonicate for 10min at room temperature, separate the produ...

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Abstract

The invention belongs to the technical field of superparamagnetism functional materials, and discloses a Cu2+-EDTA-Fe3O4 magnetic grain with the surface decorated with a large number of copper ions, a preparation method of the Cu2+-EDTA-Fe3O4 magnetic grain, and application of the Cu2+-EDTA-Fe3O4 magnetic grain in the bio-medical field. The Cu2+-EDTA-Fe3O4 magnetic grain is particularly suitable for separating histidine proteins. The magnetic grain is prepared through the following steps of preparing an EDTA-Fe3O4 magnetic grain through a hydrothermal method, adding the EDTA-Fe3O4 magnetic grain to a water-soluble copper salt solution, and obtaining the Cu2+-EDTA-Fe3O4 magnetic grain through chelation. The magnetic grain is spherical and high in dispersibility and has the grain size of about 150 nm and the magnetic saturation of 69 emu / g. Through the strong coordination of Cu2+ with which the surface of the grain is decorated and histidine residues on surfaces of hemoglobin, the superhigh adsorption capacity is achieved for hemoglobin, the adsorption capacity can reach 1250 mg / g, no obvious adsorption effect is achieved for serum albumin, excellent selectivity is expressed, and the removal efficiency is larger than 90%.

Description

technical field [0001] The invention belongs to the technical field of superparamagnetic functional materials, in particular to Cu with a large amount of copper ion modification on the surface 2+ -EDTA-Fe 3 o 4 The magnetic particle and its preparation method and application in the field of biomedicine are especially suitable for separating histidine protein. Background technique [0002] Physiological and pathological changes of the body often cause changes in blood components, so the detection of blood components has important clinical significance. Trace biomarkers in the blood are often indicators of disease. For example, alanine aminotransferase, hepatitis B surface antigen, and hepatitis C virus antibody are biological indicators for detecting liver disease, and HIV antibody is a biological indicator for AIDS detection. In addition, a series of reports point to some microRNAs in the blood as indicators of cancer. However, low-concentration biological indicators ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02H01F1/00H01F41/02C07K1/14
Inventor 贾丽丁纯
Owner SOUTH CHINA NORMAL UNIVERSITY
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