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Preparation method of sericin/mesoporous silicon composite drug-loaded nanoparticles wrapped with sericin protein

A technology of silicon nanoparticles and sericin, which is applied in the field of biomedical composite materials, achieves the effect of a simple way of obtaining raw materials, a simple operation process and good biocompatibility

Active Publication Date: 2017-08-22
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of sericin-coated mesoporous silicon to prepare drug delivery systems.

Method used

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  • Preparation method of sericin/mesoporous silicon composite drug-loaded nanoparticles wrapped with sericin protein
  • Preparation method of sericin/mesoporous silicon composite drug-loaded nanoparticles wrapped with sericin protein
  • Preparation method of sericin/mesoporous silicon composite drug-loaded nanoparticles wrapped with sericin protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Preparation of sericin / mesoporous silicon composite nanoparticles by glutaraldehyde crosslinking method provided by the present invention.

[0053] 1. Selection of silkworm cocoons:

[0054] The present invention preferably selects silkworm cocoons of silkworm silk fibroin-deficient mutant varieties (purchased from the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the Bombyx mori silk fibroin-deficient mutant variety is preserved in the National Silkworm Resource Preservation Center of the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the deposit number is 185 Nd-s ) as the raw material, the main chemical component is sericin.

[0055] 2. Extraction of sericin:

[0056] (1) Weigh 2 g cocoons of silkworm silk fibroin-deficient mutant varieties and cut them into 1 cm 2 left and right fragments;

[0057] (2) Submerge the shredded cocoons in 40 mL of 0.02 M sodium carbonate and dissol...

experiment example 1

[0066] Experimental Example 1: To study the biocompatibility of sericin / mesoporous silicon composite nanoparticles.

[0067] 1. After co-incubating the unloaded sericin / mesoporous silicon composite nanoparticles with various tumor cells for 48 hours, the cell viability was detected, such as Figure 4 Shown: When the composite system is not loaded with any drug, there is no obvious effect on the viability of the cells.

[0068] 2. After co-incubating empty sericin / mesoporous silicon composite nanoparticles with mouse-derived macrophages (Raw264.7cells) for 24 hours, the cytoskeleton and nucleus were stained with phalloidin and DAPI to observe The morphology of the cells, and the expression of macrophage inflammatory genes (TNF-α, IL-1β). Such as Figure 5 Shown in A: when macrophages were co-incubated with sericin / mesoporous silicon composite nanoparticles for 24 hours, the morphology of macrophages was similar to that of cells incubated with blank medium (negative control) f...

experiment example 2

[0069] Experimental Example 2: Detection of fluorescence properties of sericin / mesoporous silicon composite nanoparticles.

[0070] 1. Use a fluorescence spectrum tester to detect the fluorescence spectrum of sericin / mesoporous silicon composite nanoparticles, such as image 3 Shown in A: Under the excitation of an appropriate excitation wavelength, emission light with a wavelength of about 550 nm can be generated.

[0071] 2. After co-incubating sericin / mesoporous silicon composite particles with RAW264.7 macrophages for 24 hours, observe the fluorescence of the material with a fluorescence microscope, as shown in image 3 Shown in B: under the excitation of blue light source, the material can emit green fluorescence.

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Abstract

The invention relates to a mesoporous silicon nano-medicine carrier wrapped by natural sericin protein and its preparation method and application in the field of tumor treatment. The preparation method comprises: preparing pure sericin solid; preparing mesoporous silicon nano-particles; preparing regular pores amination, aldylation or disulfide mesoporous silicon nanoparticles; preparation of drug-loaded amination, aldylation or disulfide mesoporous silica nanoparticles; preparation of pH / protease or reduction / protease dual-responsive sericin / Mesoporous silicon composite multifunctional control system. The invention has the advantages of simple operation, convenient raw material acquisition method, low cost, universal application, and no need for special complex equipment and the like. The nanocomposite system prepared by this method fully utilizes the characteristics of the outer sericin protein and the inner mesoporous silicon nanoparticles, has dual responsiveness of pH / protease or reduction / protease, has the ability to inhibit tumor cells, and monitor nanoparticles in real time It has multiple functions such as localization and drug release, and has high drug loading capacity and good biocompatibility. It has broad application prospects in the field of tumor diagnosis and treatment, especially in solving the multi-drug resistance of tumors.

Description

technical field [0001] The invention relates to the field of biomedical composite materials, in particular to a variety of natural sericin-wrapped mesoporous silicon nano drug carriers, a preparation method thereof and an application in the field of tumor treatment. Background technique [0002] With the rapid development of nanoscience and technology, a variety of organic or inorganic materials have been made into nanostructures for use in the field of biomedicine, especially the use of nanomaterials as drug carriers for disease treatment has attracted more and more attention. Mesoporous silica nanoparticles (MSN), as a solid material with a honeycomb porous structure, have many excellent structural features, such as a large specific surface area and pore volume, uniform and controllable mesopore size Pores, stable physical and chemical properties, easy internal and external surface modification and good biocompatibility. Therefore, a lot of research is devoted to using me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/04A61K47/42A61K45/00A61K49/00A61P35/00
Inventor 王琳王征刘佳李琪琳徐鲁明乞超
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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