Rapid detecting method and corresponding test strip for acetamiprid residues in tea

Abstract

The invention discloses a heterogeneous-competition immunochromatographic gold-labeled test strip for detection of acetamiprid residues in tea. The test strip comprises a liner plate, a sample pad, a gold-labeled primary antibody combination pad, a nitrocellulose membrane and a water absorption pad; the gold-labeled primary antibody combination pad is coated with colloidal gold labeled acetamiprid antibodies, the nitrocellulose membrane is provided with a detection line coated with acetamiprid antigens and a quality control line coated with secondary antibodies. When the test strip is practically used, 2-3 drops of tea soup are dropwise added in a sample dropping area of the sample pad for a chromatography reaction, and 5-10 minutes later, a negative or positive result of pesticides in the sample is directly judged according to the color developing conditions of the detection line and the quality control line; the test strip is valid if the color of the quality control line is developed to be red, and the test strip is invalid if the color of the quality control line is not developed; the negative result is judged if the color of detection line is developed to be red and shows that no acetamiprid residue is detected in the tea soup, or the concentration of the acetamiprid residues in the tea soup is lower than 0.01 mg / L; the positive result is judged if the color of the detection line, namely the T line, is not developed and shows that the concentration of the acetamiprid residues in the tea soup is higher than 0.01 mg / L.

Description

technical field [0001] The invention belongs to the field of gold standard immunoassay detection of small molecule compounds, and in particular relates to a gold standard rapid test paper strip for pesticide residues based on heterologous competition immunochromatography-a rapid test paper strip suitable for acetamiprid residues in tea , the test strip is suitable for the rapid detection of acetamiprid residues of nicotinic chloride pesticides in tea samples. Background technique [0002] Since the United States, the European Union, and Japan have strengthened the quarantine and inspection of imported food and agricultural products, my country's tea export has shown a sharp decline. The main reason is that excessive pesticides have plagued my country's tea export. In the past two years, due to the high frequency of use of neonicotinoid pesticides such as acetamiprid on tea leaves, the detection rate of acetamiprid and other pesticide residues on tea leaves has been high, and...

AI Technical Summary

Problems solved by technology

So far, the common on-site rapid screening method for pesticide residues is the enzyme inhibition chromogenic disc method, but its detection rate is high, the detection object is limited to organophosphorus and carbamate pesticides, and it has a high specificity. Sex is not high

In recent years, immunochemical analysis based on antigen-antibody specific reaction has become one of the research hotspots in the rapid detection of pesticide residues. Among them, the most reported one is enzyme-linked immunosorbent assay (ELISA), and many types of pesticide ELISA have been developed. The kit does have potential market application prospects, but the detection requires multi-step reactions, is not easy to operate on-site, and still cannot truly achieve on-site rapid screening

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