Rapid detecting method and corresponding test strip for acetamiprid residues in tea

A technology of acetamiprid and test strips, which is applied in the field of pesticide residue gold standard speed test strips, can solve the problems of high detection false positive rate, inability to quickly screen on-site, detection requires multi-step reactions, etc., and achieves high detection sensitivity , good practicability, and the effect of improving sensitivity

Inactive Publication Date: 2015-12-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, the common on-site rapid screening method for pesticide residues is the enzyme inhibition chromogenic disc method, but its detection rate is high, the detection object is limited to organophosphorus and carbamate pesticides, and it has a high specificity. Sex is not high
In recent years, immunochemical analysis based on antigen-antibody specific reaction has become one of the ...

Method used

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  • Rapid detecting method and corresponding test strip for acetamiprid residues in tea
  • Rapid detecting method and corresponding test strip for acetamiprid residues in tea
  • Rapid detecting method and corresponding test strip for acetamiprid residues in tea

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: Preparation of acetamiprid gold standard speed test paper strip:

[0052] 1. Preparation of colloidal gold

[0053] Colloidal gold particles were prepared by sodium citrate (trisodium citrate) reduction method. 150mL0.01% (mass %) chloroauric acid solution is heated to boiling (about 120 ℃) ​​under oil bath condition, under magnetic stirring, add 2.5mL1% (mass %) sodium citrate aqueous solution rapidly, when the color of solution is completely When it turns bright red, continue to reflux for 5-10 minutes and then stop heating. After cooling, put the solution into a reagent bottle and store it in a refrigerator at 4°C. A colloidal gold solution with a mass concentration of 0.01% was obtained.

[0054] The colloidal gold particles were scanned in the visible light range by a UV-visible spectrophotometer, and the maximum absorption wavelength λmax was measured to be 520nm, and the absorbance value was 0.80; and the colloidal gold particles were observed und...

Embodiment 2

[0074] Example 2: Application

[0075] 1. Test strip sensitivity test

[0076] (1) Standard solution preparation

[0077] Prepare the standard stock solution of acetamiprid with methanol, the concentration of the standard stock solution is 1mg / L. Then dilute acetamiprid with 0.01MPBS buffer solution to a series concentration of standard working solution 0.1mg / L, 0.01mg / L, 0.005mg / L, 0.002mg / L.

[0078] (2) Standard solution detection

[0079] Set up 4 treatment groups, respectively blank control (0.01MPBS buffer solution), acetamiprid 0.01mg / L, 0.005mg / L, 0.002mg / L standard working solution. Use the same batch of residual speed test paper strips for detection, and repeat twice for each treatment group. The detection method is: take a tea soup sample and add 2 drops to the spotting hole for chromatographic reaction. After 5 to 10 minutes, according to the detection The color development of line 5 and quality control line 6 can directly determine the positive and negative re...

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Abstract

The invention discloses a heterogeneous-competition immunochromatographic gold-labeled test strip for detection of acetamiprid residues in tea. The test strip comprises a liner plate, a sample pad, a gold-labeled primary antibody combination pad, a nitrocellulose membrane and a water absorption pad; the gold-labeled primary antibody combination pad is coated with colloidal gold labeled acetamiprid antibodies, the nitrocellulose membrane is provided with a detection line coated with acetamiprid antigens and a quality control line coated with secondary antibodies. When the test strip is practically used, 2-3 drops of tea soup are dropwise added in a sample dropping area of the sample pad for a chromatography reaction, and 5-10 minutes later, a negative or positive result of pesticides in the sample is directly judged according to the color developing conditions of the detection line and the quality control line; the test strip is valid if the color of the quality control line is developed to be red, and the test strip is invalid if the color of the quality control line is not developed; the negative result is judged if the color of detection line is developed to be red and shows that no acetamiprid residue is detected in the tea soup, or the concentration of the acetamiprid residues in the tea soup is lower than 0.01 mg/L; the positive result is judged if the color of the detection line, namely the T line, is not developed and shows that the concentration of the acetamiprid residues in the tea soup is higher than 0.01 mg/L.

Description

technical field [0001] The invention belongs to the field of gold standard immunoassay detection of small molecule compounds, and in particular relates to a gold standard rapid test paper strip for pesticide residues based on heterologous competition immunochromatography-a rapid test paper strip suitable for acetamiprid residues in tea , the test strip is suitable for the rapid detection of acetamiprid residues of nicotinic chloride pesticides in tea samples. Background technique [0002] Since the United States, the European Union, and Japan have strengthened the quarantine and inspection of imported food and agricultural products, my country's tea export has shown a sharp decline. The main reason is that excessive pesticides have plagued my country's tea export. In the past two years, due to the high frequency of use of neonicotinoid pesticides such as acetamiprid on tea leaves, the detection rate of acetamiprid and other pesticide residues on tea leaves has been high, and...

Claims

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Application Information

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IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 赵颖杨斌郭逸蓉柳颖司芳芳方一画程敬丽朱国念
Owner ZHEJIANG UNIV
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