A kind of maca dietary fiber and its preparation method and application
A technology for dietary fiber and maca, which is applied in the field of highly active maca dietary fiber and its preparation, can solve the problem of not mentioning the utilization of maca residue and waste.
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Embodiment 1
[0061] (1) Raw material pulverization: pulverize maca root and pass through a 40-mesh sieve.
[0062] (2) Preparation: Weigh 100g of maca powder and add it to 500mL of water, add 200U / g of heat-stable α-amylase in a water bath at 90°C for 60min, cool to 30°C and then add 10U / g of For neutral protease, bathe in water at 30°C for 60 minutes, then add amyloglucosidase in an amount of 100 U / g, bathe in water at 30°C for 60 minutes.
[0063] (3) Separation and purification: add 4 volumes of 95% ethanol to the sample prepared in step (2) to precipitate overnight at room temperature, then wash with ethanol and acetone to obtain maca dietary fiber.
[0064] (4) Freeze drying.
[0065] Dietary fiber yield is 39.2% in the present embodiment.
Embodiment 2
[0067] (1) Crushing of raw materials: pulverizing the maca residue after water extraction and drying.
[0068] (2) Preparation: Weigh 100g of maca powder and add it to 5L of water, add 1000U / g of thermostable α-amylase in a water bath at 105°C for 20min, cool to 70°C and then add 100U / g of For neutral protease, bathe in water at 70°C for 20 minutes, then add amyloglucosidase in an amount of 600 U / g, bathe in water at 70°C for 60 minutes.
[0069] (3) Separation and purification: add 4 volumes of 95% ethanol to the sample prepared in step (2) to precipitate overnight at room temperature, then wash with ethanol and acetone to obtain maca dietary fiber.
[0071] Dietary fiber yield is 65.9% in the present embodiment.
Embodiment 3
[0073] (1) Raw material pulverization: maca residue after 50% ethanol extraction is pulverized.
[0074](2) Preparation: Weigh 100g of maca powder and add it to 1L of water, add 500U / g of heat-stable α-amylase in a water bath at 95°C for 30min, cool to 60°C and then add 40U / g of For neutral protease, bathe in water at 60°C for 30 minutes, then add amyloglucosidase in an amount of 250 U / g, bathe in water at 60°C for 30 minutes.
[0075] (3) Separation and purification: add 4 volumes of 95% ethanol to the sample prepared in step (2) to precipitate overnight at room temperature, then wash with ethanol and acetone to obtain maca dietary fiber.
[0076] (4) Air-dry at room temperature.
[0077] Dietary fiber yield is 45.5% in the present embodiment.
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