Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan
A technology of ginseng polysaccharides and enzymatic extraction, which is applied in the directions of metabolic diseases, fermentation, drug combination, etc., can solve the problems of large loss of active ingredients, long extraction cycle, high solvent consumption, etc., to promote release, shorten extraction time, and improve extraction efficiency Effect
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[0025] Example 1
[0026] S1. Take clean ginseng and cut it into slices with a thickness of 5 mm, and place it in a steam explosion tank. First pass nitrogen until the pressure in the steam explosion tank is 1 MPa, and explode for 15 minutes; then quickly pass steam to the pressure in the steam explosion tank It is 1.6MPa, steam explosion treatment for 1.6min, drying, and ginseng powder is obtained;
[0027] S2. Put the ginseng powder obtained in step S1 into an enzymatic hydrolysis tank, and add a biological composite enzyme preparation containing mixed protease, pectinase, glucoamylase, lignin degrading enzyme, and laccase at a ratio of 85.0 ml / ton, 35 ℃, enzymolysis under the action of high-voltage pulsed electric field for 4 hours, electric field strength 30kv / cm, 8 pulses;
[0028] S3. Heat the enzymatically hydrolyzed solution at 100°C for 6min to passivate it, add a natural source turpentine derivative with a concentration of 72.5% to extract 2.5 times at a temperature of 77....
Example Embodiment
[0030] Example 2
[0031] S1. Take clean ginseng and cut it into slices with a thickness of 5 mm, and place it in a steam explosion tank. First pass nitrogen to the steam explosion tank until the pressure in the steam explosion tank is 0.6 MPa, and explode for 7 minutes; then quickly pass steam into the steam explosion tank The pressure is 1.4MPa, steam explosion treatment is 0.6min, and dried to obtain ginseng powder;
[0032] S2. Put the ginseng powder obtained in step S1 into an enzymatic hydrolysis tank, and add a biological composite enzyme preparation containing mixed protease, pectinase, glucoamylase, lignin degrading enzyme, and laccase at a ratio of 70.0 ml / ton, 30 ℃, enzymolysis under the action of high-voltage pulsed electric field for 3 to 5 hours, electric field intensity 25kv / cm, number of pulses 6;
[0033] S3. Heat the enzymatically hydrolyzed solution at 100°C for 5 minutes to inactivate it, add natural source turpentine derivatives with a concentration of 65%, and ...
Example Embodiment
[0035] Example 3
[0036] S1. Take clean ginseng and cut it into slices with a thickness of 5 mm, and place it in a steam explosion tank. First pass nitrogen until the pressure in the steam explosion tank is 1.4MPa, and explode for 23 minutes; then quickly pass steam into the steam explosion tank The pressure is 1.8MPa, steam explosion treatment is 2.6min, and it is dried to obtain ginseng powder;
[0037] S2. Put the ginseng powder obtained in step S1 into an enzyme hydrolysis tank, and add a biological composite enzyme preparation containing mixed protease, pectinase, glucoamylase, lignin degrading enzyme, and laccase at a ratio of 100.0 ml / ton, 40 ℃, enzymatic hydrolysis under the action of high-voltage pulsed electric field for 5 hours, electric field intensity 35kv / cm, pulse number 10;
[0038] S3. Heat the enzymatically hydrolyzed solution at 100°C for 7 minutes to passivate it, add a natural source turpentine derivative with a concentration of 80%, and extract 3 times. The ex...
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