Preparation method of dandelion extract

Abstract

The invention relates to a preparation method of a dandelion extract. The preparation method comprises the following steps of water extraction, decompress concentration, alcohol precipitation, pH value regulation, macroporous adsorption resin purification, concentration, drying and the like. A technology is simple and easy to operate, used macroporous resin can be regenerated and reused, preparation cost is low, and environmental pollution is low; an ethanol solution serves as an extracting and eluting solvent, and is high in safety, low in price, easy to obtain and low in production cost; and the obtained dandelion extract has high contents of total flavones and cafeic acid, has complete ingredients, not only contains effective ingredients such as flavones and phenolic acids, but also contains ingredients such as coumarin and esters, wherein the content of the total flavones in medicinal materials is increased from 2% to be above 50%, and the content of the cafeic acid is increased from 0.02% to be above 0.8%.

Description

technical field [0001] The invention relates to a preparation method of a dandelion extract, which belongs to the field of traditional Chinese medicines. Background technique [0002] Dandelion is the dry whole herb of Taraxacum mongolicum Hand.-Mazz., TaraxacumborealisinenseKitm. or several plants of the same genus, which are distributed all over the country. It is excavated when the flowers first bloom from spring to autumn, removed impurities, washed, dried in the sun, and used fresh or raw. Dandelion is a commonly used traditional Chinese medicine in clinical practice. "Compendium of Materia Medica" says that it "specializes in treating carbuncles and poisons, and is also a wonderful product for treating stranguria." Ancient prescriptions record that dandelion is "an important medicine for relieving heat and cooling blood" and "it is cheap but has great merit. ". Dandelion is bitter and sweet in taste, cold in nature, and returns to the liver and stomach meridians. It...

AI Technical Summary

Problems solved by technology

At present, the separation and purification of medicinal components of dandelion are mainly based on a single component. For example, Chinese patent CN103070899A provides a method for separation and purification of total flavonoids of dandelion, which specifically includes methanol reflux extraction, concentration, pH adjustment, and macroporous resin column separation. , silica gel chromatography column separation and other steps to prepare the total flavonoids with a content of more than 96%; but this method uses 85%-100% methanol extraction, and then uses methanol, acetone and their mixed solution as eluent for separation and purification. It is easy to cause toxic solvent residues in the extract, and this method uses a macroporous resin column and a silica gel chromatography column for separation and purification at the same time, and the operation steps are numerous, which is not suitable for large-scale production

Chinese patent CN103494858A discloses a macroporous resin enrichment method for total flavonoids of dandelion, including degreasing of petroleum ether, ethanol reflux extraction, concentration, pH adjustment, separation and purification of macroporous resin column, etc., obtaining total flavonoids with a content of more than 60%. Flavonoids, but this method is degreased with petroleum ether before extraction, which is easy to cause residues of organic solvents, and is not suitable for large-scale production

Although total flavonoids and phenolic acids are one of the medicinal components of dandelion, only using total flavonoids or total phenolic acids as indicators cannot fully reflect the medicinal effect of dandelion extracts

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