Production technology for high-density fermentation of lactics and separation coupling of lactic acid by physical method

A technology of high-density fermentation and lactic acid physics, which is applied in the production process field of high-density fermentation of lactic acid bacteria and the separation and coupling of lactic acid physical method, can solve the problems such as the inability to fully meet the production requirements of high-density fermentation and the toxicity of lactic acid bacteria, and improve the yield of bacteria. and specific product yield, convenient operation and high production efficiency

Inactive Publication Date: 2016-02-03
BIOGROWING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method will increase a large amount of Na+ or NH4+ in the fermentation broth system, and there will still be a large amount of lactate or molecular lactic acid. This chemical method cannot fully meet the production requirements of high-density fermentation, and it is reported that lactate or molecular lactic acid Molecular lactic acid is also toxic to lactic acid bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Streptococcus thermophilus strain preparation: Streptococcus thermophilus strains are preserved by the strain preservation bank. Streptococcus thermophilus strains were activated by culturing in liquid medium at 35°C for 16 hours, then transferred to fermentation medium to form fermentation broth, the inoculation amount was 2% (v / v), and the fermentation period was 60 hours.

[0025] The liquid medium is 20g / L glucose, 3g / L yeast extract, 10g / L peptone, 2g / L anhydrous sodium acetate, 2g / L dipotassium hydrogen phosphate, 0.3g / L MgSO 4 ·7H 2 O, 0.03g / L MnSO 4 ·H 2 O,, sterilized at 121°C for 20 minutes and then cooled to room temperature.

[0026] The fermentation medium is 100g / L sucrose, 30g / L yeast extract, 10g / L peptone, 2g / L sodium sulfate, 2g / L anhydrous sodium acetate, 2g / L dipotassium hydrogen phosphate, 0.3 g / L of MgSO 4 ·7H 2 O, 0.03g / L MnSO 4 ·H 2 After mixing, sterilize at 121°C for 20 minutes and cool to room temperature. The nitrogen pressure is mai...

Embodiment 2

[0034] Streptococcus thermophilus strain preparation: Streptococcus thermophilus strains are preserved by the strain preservation bank. Streptococcus thermophilus strains were activated by culturing in liquid medium at 37.5°C for 8 hours, then transferred into fermentation medium to form fermentation broth, the inoculation amount was 4% (v / v), and the fermentation period was 60 hours.

[0035] The liquid medium is 20g / L glucose, 5g / L yeast extract, 5g / L peptone, 2g / L anhydrous sodium acetate, 2g / L dipotassium hydrogen phosphate, 0.1g / L MgSO 4 ·7H 2 O, 0.03g / L MnSO 4 ·H 2 O, sterilized at 121°C for 20 minutes and then cooled to room temperature.

[0036] The fermentation medium is 100g / L sucrose, 5g / L yeast extract, 10g / L peptone, 10g / L beef extract powder, 2g / L sodium sulfate, 2g / L anhydrous sodium acetate, 2g / L L dipotassium hydrogen phosphate, 0.1g / L MgSO 4 ·7H 2 O, 0.03g / L MnSO 4 ·H 2 After mixing, sterilize at 121°C for 20 minutes and cool to room temperature. The...

Embodiment 3

[0044] Streptococcus thermophilus strain preparation: Streptococcus thermophilus strains are preserved by the strain preservation bank. Streptococcus thermophilus strains were activated by culturing in liquid medium at 39°C for 20 hours, then transferred to fermentation medium to form a fermentation broth, the inoculation amount was 3% (v / v), and the fermentation period was 60 hours.

[0045] The liquid medium is 20g / L glucose, 5g / L yeast extract, 10g / L peptone, 2g / L anhydrous sodium acetate, 2g / L dipotassium hydrogen phosphate, 0.1g / L MgSO 4 ·7H 2 O, 0.03g / L MnSO 4 ·H 2 O, sterilized at 121°C for 20 minutes and then cooled to room temperature.

[0046] The fermentation medium is 100g / L sucrose, 5g / L yeast extract, 10g / L peptone, 10g / L beef extract powder, 2g / L sodium sulfate, 2g / L anhydrous sodium acetate, 2g / L L dipotassium hydrogen phosphate, 0.1g / L MgSO 4 ·7H 2 O, 0.03g / L MnSO 4 ·H 2 After mixing, sterilize at 121°C for 20 minutes and cool to room temperature. The...

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PUM

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Abstract

The invention relates to the technical field of inocula preparation, and concretely relates to a production technology for high-density fermentation of lactics and separation coupling of lactic acid by a physical method. Compared with the prior art, through lactics high density fermentation and lactic acid separating by ion exchange resin, feedback inhibition of a lactic acid metabolite can be released, through a high density broth obtained in the technology, bacterium concentration can reach 30g / L, in the prepared freeze-dried powder, live bacteria number is more than 8.0*1011 cfu / g, a culture volume is reduced, a production period is shortened, and a thalline yield and a specific products yield are increased. According to the invention, thalline growth inhibition can be released by employing the ion exchange resin for lactic acid adsorption, and production cost is reduced through subsequent elution and recovery. In addition, the ion exchange resin has the advantages of high selectivity, large exchange capacity, simple operation and easy automation, so that the production technology has the advantages of simpliness, more-convenient operation and high production efficiency.

Description

technical field [0001] The invention relates to the technical field of microbial agent preparation, in particular to a production process of high-density fermentation of lactic acid bacteria and separation and coupling of lactic acid physical methods. Background technique [0002] Yogurt is a dairy product that is based on milk and fermented by Streptococcus thermophilus and Lactobacillus bulgaricus. Now the production of yogurt mostly uses direct-into-type starters, because the number and activity of lactic acid bacteria cells significantly affect the performance of starters. [0003] Lactic acid bacteria themselves cannot synthesize various vitamins and amino acids, and have very strict nutritional requirements. When cultivating lactic acid bacteria on a large scale, it is necessary to add substances rich in growth factors such as vitamins and amino acids to facilitate the proliferation of lactic acid bacteria. [0004] During the growth process, lactic acid bacteria gra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C07C59/08C07C51/47C12R1/46
Inventor 马霞姬彬宋锦安刘帅
Owner BIOGROWING CO LTD
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