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Preparation method of electrochemical sensor for detection of homocysteine

A homocysteine ​​and electrochemical technology, applied in the field of preparation of electrochemical sensors for rapid detection of homocysteine, can solve the problems of complex pretreatment, high cost, and low sensitivity

Active Publication Date: 2016-02-17
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many of the above methods have disadvantages such as complex pretreatment, high cost, and low sensitivity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Put the pretreated glassy carbon electrode into the phosphate buffer solution with pH=7.0, and carry out cyclic scanning treatment in the potential range of -0.2V~+2.0V at the scanning rate of 20mV / s for 10min.

[0015] Then put the electrode into the aqueous solution containing 0.1M pyrrole monomer, 0.0005M phenanthrene quinone sulfonate, 0.5mg / ml graphene oxide, pass N 2 After 30 min, the three-electrode system was used for constant current electrodeposition, and the applied current was 0.5mAcm -2 , the electric quantity of electrodeposition is 1.2 coulombs; After washing the obtained polypyrrole film, it is placed in a three-electrode system for electrochemical reduction, the applied voltage is 1.1V, the electrolyte is PBS buffer solution, and after the reaction, it is washed repeatedly with water. Polypyrrole / phenanthrene quinone sulfonate / graphene composite modified electrode can be obtained;

[0016] The above-mentioned conductive polymer modified electrode was p...

Embodiment 2

[0018] Put the pretreated glassy carbon electrode into the phosphate buffer solution with pH=5.0, and perform cyclic scanning treatment in the potential range of -0.2V~+1.0V at a scanning rate of 15mV / s for 15min;

[0019] Then put the electrode into the aqueous solution containing 0.2M pyrrole monomer, 0.0005M anthraquinone sulfonate, 1.0mg / ml graphene oxide, pass N 2 After 30 min, the three-electrode system was used for constant current electrodeposition, and the applied current was 2mAcm -2 , the electric quantity of electrodeposition is 2.0 coulombs; After washing the obtained polypyrrole film, it is placed in a three-electrode system for electrochemical reduction, the applied voltage is 1.2V, the electrolyte is PBS buffer solution, and after the reaction, it is washed repeatedly with water. Polypyrrole / anthraquinone sulfonate / graphene composite modified electrode can be obtained;

[0020] The above-mentioned conductive polymer modified electrode was placed in an 8×10 -5...

Embodiment 3

[0022] Put the pretreated glassy carbon electrode into the phosphate buffer solution with pH = 7.0, and perform cyclic scanning treatment in the potential range of -0.2V to +2.0V at a scanning rate of 20mV / s for 50min;

[0023] Then put the electrode into an aqueous solution containing 0.05M aniline monomer, 0.1M phenanthrene quinone sulfonate, 1.0mg / ml graphene oxide, and pass N 2 After 30 min, the three-electrode system was used for constant current electrodeposition, and the applied current was 1mAcm -2 , the electric quantity of electrodeposition is 1.6 coulombs; After washing the obtained polyaniline film, it is placed in a three-electrode system for electrochemical reduction, the applied voltage is 1.0V, the electrolyte is PBS buffer solution, and after the reaction, it is washed repeatedly with water. Polyaniline / phenanthrene quinone sulfonate / graphene composite modified electrode can be obtained;

[0024] The above-mentioned conductive polymer modified electrode was p...

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PUM

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Abstract

The invention provides a preparation method of an electrochemical sensor for detection of homocysteine. The preparation method comprises preparation of a conducting polymer modified electrode and preparation of a poly(methyl violet) modified conducting polymer electrochemical sensor. The conducting polymer modified electrode is put into a phosphoric acid buffer solution containing 8*10<-5> mol / L poly(methyl violet) and subjected to electrolytic deposition for five minutes under +1.6V, then the scanned conducting polymer modified electrode is taken out and washed several times with absolute ethanol and secondary distilled water sequentially, and the poly(methyl violet) modified conducting polymer electrochemical sensor is prepared. The preparation method of the sensor is simple, and the sensitivity is high.

Description

technical field [0001] The invention relates to an electrochemical sensor, in particular to a method for preparing an electrochemical sensor for rapid detection of homocysteine. Background technique [0002] Homocysteine, also known as homocysteine, is a thiol-containing amino acid. In recent years, medical research has confirmed that changes in the content of homocysteine ​​in plasma are associated with increased incidence of coronary heart disease and cerebrovascular disease. close relationship. Therefore, detecting the level of homocysteine ​​is of great significance to the pathological research of related diseases. [0003] At present, a variety of analytical methods have been established for the detection of homocysteine, such as photometric detection, ultraviolet spectrophotometry, fluorescence, spectrometry, electrochemical detection and enzyme immunoassay. However, many of the above methods have disadvantages such as complex pretreatment, high cost, and low sensiti...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 何丹农王丹张春明吴晓燕
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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