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Poly-L-lactide-caprolactone copolymer (PLCL) three-dimensional porous scaffold, PLCL and collagen (PLCL-COL) composite scaffold and preparation methods of scaffolds

A three-dimensional porous, composite scaffold technology, applied in the field of biomedicine, can solve the problems of unsuitable low-temperature rapid prototyping, preparation of scaffolds, poor cell adhesion, etc., achieve good three-dimensional structure and porosity, make up for the lack of performance, and improve hydrophilicity Effect

Inactive Publication Date: 2016-02-24
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing a PLCL three-dimensional porous scaffold, aiming to solve the problem that PLCL is limited in its application in cartilage tissue engineering due to its strong surface hydrophobicity and poor cell adhesion, and it is not suitable for low-temperature rapid prototyping technology Problems with preparing scaffolds
[0007] Another object of the present invention is to provide a preparation method of PLCL-COL composite scaffold, which solves the problem that PLCL materials are limited in their application in cartilage tissue engineering due to their strong surface hydrophobicity and poor cell adhesion, and are not suitable for low-temperature rapid prototyping Problems with technical preparation of stents

Method used

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  • Poly-L-lactide-caprolactone copolymer (PLCL) three-dimensional porous scaffold, PLCL and collagen (PLCL-COL) composite scaffold and preparation methods of scaffolds
  • Poly-L-lactide-caprolactone copolymer (PLCL) three-dimensional porous scaffold, PLCL and collagen (PLCL-COL) composite scaffold and preparation methods of scaffolds
  • Poly-L-lactide-caprolactone copolymer (PLCL) three-dimensional porous scaffold, PLCL and collagen (PLCL-COL) composite scaffold and preparation methods of scaffolds

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preparation example Construction

[0035] In view of this, an embodiment of the present invention provides a method for preparing a PLCL three-dimensional porous scaffold. The PLCL three-dimensional porous scaffold is prepared by low-temperature deposition manufacturing technology, which specifically includes the following steps:

[0036] S01. CAD software is used for modeling preprocessing, Aurora software is used for layered slicing, and Cark software is used to design the printing parameters of PLCL three-dimensional porous scaffolds;

[0037] S02. Dissolving PLCL in an organic solvent to prepare a PLCL homogenate;

[0038] S03. When the temperature of the molding chamber drops to -25 to -35°C, apply the organic solvent on the molding platform, use the Cark software to set the modeling parameters, and then start printing and modeling to obtain a preformed PLCL three-dimensional porous scaffold;

[0039] S04. The PLCL three-dimensional porous scaffold preform is taken out and then freeze-dried to obtain a PLCL ...

Embodiment 1

[0068] A method for preparing a PLCL three-dimensional porous scaffold, the PLCL three-dimensional porous scaffold is prepared by low-temperature deposition manufacturing technology, specifically comprising the following steps:

[0069] S11. Modeling, layering and stacking of stents aided by computer: first, a model in STL file format is generated through CAD software modeling preprocessing; then use Aurora software to import the STL file and perform layered slices on it to generate A CLI file; finally, use the Cark software of the low-temperature rapid prototyping system to open the CLI file, design the printing parameters of the bracket and print it. CAD software was used for modeling preprocessing, Aurora software for layered slicing, and Cark software was used to design the printing parameters of PLCL three-dimensional porous scaffolds;

[0070] S12. Add PLCL accurately weighed by a high-precision electronic balance and a certain amount of 1,4-dioxane in the beaker, so tha...

Embodiment 2

[0074] A preparation method of PLCL-COL composite support, comprising the following steps:

[0075] Q21. Provide the PLCL three-dimensional porous scaffold prepared by the above method;

[0076] Q22. Place the PLCL three-dimensional porous scaffold in 0.2% NaOH ethanol solution for 10 minutes, take it out, soak it in distilled water, and rinse it to obtain the first sample;

[0077] Q23. Place the first sample in 0.5% collagen solution (0.5g collagen dissolved in 100mL 0.3% acetic acid solution) for 4 hours, soak in distilled water, rinse the excess collagen solution, and obtain the PLCL-COL composite stent preform;

[0078] Q24. Freeze-drying the PLCL-COL composite scaffold preform to prepare the PLCL-COL composite scaffold.

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Abstract

The invention is applicable to the field of biomedicine, and provides a poly-L-lactide-caprolactone copolymer (PLCL) three-dimensional porous scaffold, a PLCL and collagen (PLCL-COL) composite scaffold and preparation methods of the scaffolds. The preparation method of the PLCL three-dimensional porous scaffold, on the basis of a low-temperature deposition manufacturing technology, specifically comprises the following steps: implementing modeling pretreatment by virtue of CAD software, conducting hierarchical slicing by virtue of Aurora software, and implementing design of printing parameters of the PLCL three-dimensional porous scaffold by virtue of Cark software; dissolving the PLCL in an organic solvent, so that a PLCL homogenous liquid is prepared; smearing the organic solvent on a forming platform when forming room temperature drops to minus 25-35 DEG C, and printing and shaping after setting the shaping parameters by virtue of the Cark software, so that a PLCL three-dimensional porous scaffold prefabricated part is obtained; and taking out and freeze-drying the PLCL three-dimensional porous scaffold prefabricated part, so that the PLCL three-dimensional porous scaffold is obtained.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a PLCL three-dimensional porous scaffold, a PLCL-COL composite scaffold and a preparation method thereof. Background technique [0002] Articular cartilage defects caused by various traumas, sports injuries, bone diseases, etc. are common in clinical practice. Due to the relatively weak ability of chondrocytes to divide and proliferate, lack of nutrient vessels and other factors, articular cartilage repair has always been a clinical problem. Cartilage defects often lead to joint degeneration, eventually forming osteoarthritis, manifested as joint pain and limitation of motion, affecting people's quality of life. The emergence of cartilage tissue engineering in recent years is expected to solve this problem. [0003] Scaffold, as one of the three cores of cartilage tissue engineering, can be temporarily used as extracellular matrix (ECM), providing a scaffolding environment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/56A61L27/58
CPCA61L27/48A61L27/56A61L27/58A61L2430/06C08L89/06A61L31/129C08L67/04
Inventor 刘威王大平关炼雄王大明朱伟民段莉陈洁琳
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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