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Crosslinked hyaluronic acid dry powder as well as preparation method and application thereof

A technology of cross-linking hyaluronic acid and hyaluronic acid, which is applied in the fields of pharmaceutical formulations, cosmetic preparations, toiletry preparations, etc., and can solve the problem that dialysis operation is not easy for large-scale industrial production, the degradation of hyaluronic acid long-chain breaks, and the impact Gel use effect and other issues, to achieve the effect suitable for large-scale production, thickening and skin feeling, and convenient for clinical use

Active Publication Date: 2016-06-15
BLOOMAGE BIOTECHNOLOGY CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the reaction system of this method, the hyaluronic acid is dissolved in the lye and directly reacted with the crosslinking agent, which will cause the breakage and degradation of the long chain of hyaluronic acid and affect the crosslinking effect.
At the same time, what is obtained in the middle of this method is a wet precipitate containing a large amount of organic solvent and water. Although dialysis is performed after reconstitution, a relatively large amount of organic solvent will still remain in the obtained cross-linked hyaluronic acid gel, which will affect the use effect of the gel.
And dialysis operation is not easy for large-scale industrial production

Method used

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  • Crosslinked hyaluronic acid dry powder as well as preparation method and application thereof
  • Crosslinked hyaluronic acid dry powder as well as preparation method and application thereof
  • Crosslinked hyaluronic acid dry powder as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Accurately take 5 μl of 1,4-butanediol diglycidyl ether and add it to 100 mL of sodium chloride solution (50 mg / mL). After mixing, weigh 1 g of sodium hyaluronate (molecular weight 1.5 million Da) and dissolve it in the above solution. After the sodium hyaluronate is fully dissolved, gradually add 186ml of ethanol until the sodium hyaluronate precipitates a white precipitate. After the whole reaction system showed a solid-liquid suspension system, it was stirred and reacted at 30° C. for 6 hours. Then let stand and discard the supernatant. Finally, wash and dehydrate with ethanol for 3 times, then suction filter and discard the filtrate, put the wet powder in a vacuum drying oven at 25°C and dry it to make a dry cross-linked sodium hyaluronate powder. Weigh 0.2 g of the dry powder and add it to 100 ml of purified water to dissolve, and as a result, a colorless, transparent, smooth and viscous solution is obtained, with a kinetic viscosity of 350 mPa s, which is higher ...

Embodiment 2

[0040]Accurately take 10 μl of 1,4-butanediol diglycidyl ether and add it to 100 mL of sodium chloride solution (50 mg / mL). After mixing, weigh 1 g of sodium hyaluronate (molecular weight 1.5 million Da) and dissolve it in the above solution. After the sodium hyaluronate is fully dissolved, gradually add 186ml of ethanol until the sodium hyaluronate precipitates a white precipitate. After the whole reaction system showed a solid-liquid suspension system, it was stirred and reacted at 30° C. for 6 hours. Then let stand and discard the supernatant. Finally, it was washed and dehydrated with ethanol for 3 times, and the filtrate was discarded by suction filtration. The wet powder was dried in a vacuum drying oven at 30°C to prepare a dry cross-linked sodium hyaluronate powder. Weigh 0.2 g of the dry powder and add it to 100 ml of purified water to dissolve, and as a result, a colorless, transparent, smooth and viscous solution is obtained, with a dynamic viscosity of 1020 mPa s,...

Embodiment 3

[0042] Accurately take 20μl of 1,4-butanediol diglycidyl ether and add it to 100mL of sodium chloride solution (50mg / mL). After the sodium hyaluronate is fully dissolved, gradually add 186ml of ethanol until the sodium hyaluronate precipitates a white precipitate. After the whole reaction system showed a solid-liquid suspension system, it was stirred and reacted at 30° C. for 6 hours. Then let stand and discard the supernatant. Finally, it was washed and dehydrated with ethanol for 3 times, and the filtrate was discarded by suction filtration. The wet powder was dried in a vacuum drying oven at 40°C to prepare a dry cross-linked sodium hyaluronate powder. Weigh 0.2 g of the dry powder and add it to 100 ml of purified water to dissolve, and as a result, a colorless, viscous solution with transparent particles is obtained, and its dynamic viscosity is 7800 mPa s, which is higher than that of the 0.2% initial hyaluronic acid (molecular weight: 1.5 million Da) solution dynamic v...

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Abstract

The invention relates to the technical field of hyaluronic acid, in particular to crosslinked hyaluronic acid dry powder. Hyaluronic acid is dissolved in an inorganic salt water solution premixed with a crosslinking agent; an organic solvent is added, precipitates are separated out from the hyaluronic acid, a solid-liquid suspension system is formed, and a cross-linking reaction is performed; an organic solvent is added for washing the precipitates; vacuum drying is performed, and the crosslinked hyaluronic acid dry powder is obtained. The crosslinking reaction is performed in the solid-liquid suspension state, and the gelling property is better; crosslinked hyaluronic acid gels with different concentrations can be prepared from the obtained crosslinked hyaluronic acid dry powder, and use is facilitated. After the obtained crosslinked hyaluronic acid dry powder is dissolved in water, colorless transparent gel is formed, and the crosslinked hyaluronic acid dry powder has the effects that the viscoelasticity is excellent, the dry powder can perform the function of long-acting moisture retention after being applied to cosmetics, physical wrinkle filling is realized, active ingredients are released slowly, the cosmetics cosmetic is thickened, the skin feeling during use is improved and the like.

Description

technical field [0001] The invention relates to the technical field of hyaluronic acid, in particular to a dry powder of cross-linked hyaluronic acid, as well as its preparation method and application. Background technique [0002] Hyaluronic acid is widely distributed in various parts of the human body, and the skin also contains a large amount of hyaluronic acid. As the human body ages, the content of hyaluronic acid in the skin gradually decreases. Hyaluronic acid has been widely used in cosmetics. It can improve skin nutrition metabolism, make skin soft, smooth, wrinkle-free, increase elasticity, and prevent aging. It is also a good transdermal absorption accelerator while moisturizing. However, when the amount is added in a large amount, it is easy to draw and get muddy during the application process, and the skin after application sweats or is in a relatively humid environment, and is prone to stickiness. Cross-linked or modified hyaluronic acid has different rheolog...

Claims

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Application Information

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IPC IPC(8): C08J3/24A61K8/73A61K47/36A61L27/20A61L27/52A61Q19/00A61Q19/08C08L5/08
CPCA61K8/735A61K47/36A61L27/20A61L27/52A61L2400/06A61Q19/00A61Q19/08C08J3/24C08J3/247C08J2305/08
Inventor 王冠凤郭学平石艳丽魏健钱晓路王成山耿凤
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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