Glucoamylase and nanogold co-doped carbon nanomaterial hybrid anode-based starch biofuel cell

A technology of biofuel cells and carbon nanomaterials, applied in the direction of regenerative fuel cells, fuel cells, battery electrodes, etc., can solve the problems of incomplete oxidation substrates, short battery life, difficult to maintain enzyme activity, etc., to achieve excellent operation and The effect of storage stability

Active Publication Date: 2016-07-06
青岛博亭科技有限公司
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Problems solved by technology

However, due to the difficulty of maintaining the activity of enzymes in vitro, the stability is relatively low, the battery life is relatively short, and usually the substrate of enzymes is single, resulting in only partial oxidation of biofuels.
[0004] In the application of enzyme-based biofuel cells, although enzymes exhibit high-efficiency catalytic activity under mild conditions, their singleness of substrates, incomplete oxidation of substrates, and poor long-term stability are all problems. It has become a key factor restricting the further development of enzyme-based biofuel cells in the application field
In order to increase the energy density and expand the range of biofuels that can be used, the strategy of multi-enzyme system has been widely reported by researchers. However, due to the more complex electron transfer mechanism in the multi-enzyme system, the activities of different enzymes are restricted by each other. As a result, there are still huge challenges in the research and practical application of the system

Method used

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  • Glucoamylase and nanogold co-doped carbon nanomaterial hybrid anode-based starch biofuel cell
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  • Glucoamylase and nanogold co-doped carbon nanomaterial hybrid anode-based starch biofuel cell

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Experimental program
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Embodiment 1

[0036] 1.1 Reagents:

[0037] Glucoamylase (GA); N-hydroxysuccinimide ester 1-pyrene butyric acid (pyrenebutanoic acid succinimidylester, PASE); trihydrate chloroauric acid (HAuCl 4 ·3H 2 O); carbon nanofibers (CNFs); dimethylformamide (Dimethylformamide); phosphate buffer saline pH7.4 (PBSbuffer); phosphate buffer saline pH5.5 (PBSbuffer).

[0038] 1.2 Preparation of carbon nanofiber-modified glassy carbon electrodes (AuNPs / CNFs / GCE) deposited on the surface of AuNPs

[0039] 1) The glassy carbon electrode with a working area of ​​0.3 mm in diameter was thoroughly ground and polished with alumina powders of 0.3 and 0.05 microns in diameter, and then ultrasonically cleaned in absolute ethanol and deionized water, and then dried with nitrogen gas for later use.

[0040] 2) 6-8 μl of CNFs dispersed in DMF solvent with a concentration of 3mg / ml was drop-coated on the surface of the glassy carbon electrode, and the electrode was dried in a 37°C convection oven for two hours.

...

Embodiment 2

[0050] 2.1 Anode structure characterization and performance

[0051] In order to achieve efficient electrochemical catalysis of glucose on the electrode surface under neutral solution conditions, a pure gold nanoparticle surface with a large number of catalytically active sites on the surface is indispensable. Therefore, at first the present invention adopts the method of direct electrochemical deposition to modify the surface of CNFs without any protective groups, pure AuNPs on the surface, characterized by scanning electron microscopy ( figure 2 A) It can be seen that the prepared AuNPs, with a diameter of about 8-13nm, are uniformly distributed on the surface of CNFs. At the same time, it can be seen that the electrode surface modified by CNFs is a three-dimensional cross network structure with micro-nano holes, which can not only effectively improve the catalyst loading, but also facilitate the solution diffusion and mass transfer process on the electrode surface. In add...

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Abstract

The invention discloses a glucoamylase and nanogold co-doped carbon nanomaterial hybrid anode-based starch biofuel cell. Through combination of efficient hydrolysis of GA on starch and efficient electrochemical catalytic oxidation of electrochemically deposited AuNPs on glucose, the starch biofuel cell based on a hybrid anode which is co-modified by direct electrochemical deposition of the AuNPs and crosslinking of the GA and a carbon nanomaterial is successfully prepared. The assembled fuel cell has the maximal output power which can reach 91.4[mu]W / cm<2>, and excellent operation and storage stability. Furthermore, the strategy for constructing a cascade reaction anode by enzyme and precious metal in a hybrid way for working can be taken as a common method, and can be developed and applied to research of various polysaccharide fuel cells comprising the glucose oxidation process in future.

Description

technical field [0001] The invention belongs to the field of biofuel cells, and relates to a starch biofuel cell, in particular to a starch biofuel cell based on a carbon nanomaterial hybrid anode co-modified by glucoamylase and nano gold. Background technique [0002] With the energy crisis of depletion of fossil energy and environmental problems facing the development of the world today, the research on the development and utilization of sustainable green energy has become the focus of domestic and foreign researchers. A biofuel cell is a special fuel cell that uses natural microorganisms or enzymes as a catalyst to directly convert chemical energy in fuel into electrical energy. It not only has the advantages of high efficiency and no pollution of traditional fuel cells, but also has the following characteristics: first, a wide range of fuel sources, and renewable organic substances such as glucose, xylose, and starch that exist in large quantities in nature can be used a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01M4/86H01M4/88H01M4/90H01M8/18
CPCH01M4/8853H01M4/9083H01M8/188H01M2004/8689Y02E60/50
Inventor 赵凯夏霖王宗花夏建飞金辉
Owner 青岛博亭科技有限公司
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