Culture method capable of increasing the flavonoid content and varieties of auricularia auricula
The technology of black fungus and culture medium is applied in the cultivation field of improving the content and types of black fungus flavonoids, can solve the problems of a large amount of wood materials, less research on flavonoids, waste of resources and the like, and achieves reduction of wood usage, good development prospects, The effect of promoting
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Embodiment 1
[0075] 1. Preparation of functional extractant:
[0076] (1) Water is the extractant, the mass ratio of black fungus bran to water, that is, the solid-to-liquid ratio is 1g:4mL, the pH is 4.5, the extraction time is 120s, the extraction voltage is 220V, the extraction temperature is 30°C, in a flash extractor Rapid extraction within the cellulase and xylanase-containing extracts were obtained, and the measured enzyme activities were 8IU / g and 8IU / g respectively for use. Among them, the enzyme activity of cellulase refers to 1 enzyme activity unit, which means that under specific conditions (25°C, other conditions are optimal), 1 micromole of sodium carboxymethylcellulose can be converted within 1 minute. The amount of enzyme required for cellobiose and glucose; the enzyme activity of xylanase refers to 1 enzyme activity unit, which means that under specific conditions (35 ° C, other optimal conditions), it can The amount of enzyme required to convert 1 micromol of the substra...
Embodiment 2
[0092] 1. Preparation of functional extractant:
[0093] (1) Water is the extractant, the solid-to-liquid ratio of Auricularia black fungus bran is 1g:1mL, the pH is 4.7, the extraction time is 200s, the extraction voltage is 220v, the extraction temperature is 25°C, and it is extracted quickly in a flash extractor to obtain cellulose-containing Enzyme and xylanase extract, measuring enzyme activity (definition is the same as embodiment 1) is 10IU / g, and 10IU / g is subsequent use;
[0094] (2) 20 parts of physalis stems; 20 parts of red leaves in the mountains; 5 parts of chrysanthemum, 5 parts of dandelion, 5 parts of orange peel, 10 parts of Acanthopanax, 10 parts of black currant, 5 parts of Sophora japonica, 10 parts of Schisandra, 10 parts of tartary buckwheat sticks. Crush, pass through a 20-mesh sieve, mix well and set aside.
[0095] (3) Add 10ml of the enzyme solution extracted in (1) to every 100g of the solid in (2); use 40% ethanol and water- as the solvent, the s...
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