Culture method capable of increasing the flavonoid content and varieties of auricularia auricula

The technology of black fungus and culture medium is applied in the cultivation field of improving the content and types of black fungus flavonoids, can solve the problems of a large amount of wood materials, less research on flavonoids, waste of resources and the like, and achieves reduction of wood usage, good development prospects, The effect of promoting

Active Publication Date: 2016-07-20
MUDANJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] People focus on the key functional components of black fungus, such as proteins, polysaccharides, and pigments. The polysaccharides can lower blood sugar, lower fat, inhibit platelet aggregation, and resist thrombosis. In the study of extraction process, there is basically no research on the types of flavonoids of black fungus. The reason is that the rich protein, polysaccharide and gum components of black fungus affect its extraction process. At the same time, the content of flavonoids is relatively low at 3-5mg / 100g, so its Research lags behind
At the same time, in the process of cultivating black fungus, although we have seen researches on cultivating black fungus with woody materials such as Acanthopanax and seabuckthorn, such as the enrichment of components such as eleutheroside, seabuckthorn flavonoids and black fungus, etc., but the increase in flavonoids has not been solved. It requires a large amount of related wood materials at the same time, and the cost is unreasonable. Generally, the branches and leaves rich in related components are discarded, and there is a waste of resources. The most important thing is that due to factors such as resource distribution, it is not suitable for large-scale promotion.

Method used

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  • Culture method capable of increasing the flavonoid content and varieties of auricularia auricula
  • Culture method capable of increasing the flavonoid content and varieties of auricularia auricula
  • Culture method capable of increasing the flavonoid content and varieties of auricularia auricula

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Experimental program
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Effect test

Embodiment 1

[0075] 1. Preparation of functional extractant:

[0076] (1) Water is the extractant, the mass ratio of black fungus bran to water, that is, the solid-to-liquid ratio is 1g:4mL, the pH is 4.5, the extraction time is 120s, the extraction voltage is 220V, the extraction temperature is 30°C, in a flash extractor Rapid extraction within the cellulase and xylanase-containing extracts were obtained, and the measured enzyme activities were 8IU / g and 8IU / g respectively for use. Among them, the enzyme activity of cellulase refers to 1 enzyme activity unit, which means that under specific conditions (25°C, other conditions are optimal), 1 micromole of sodium carboxymethylcellulose can be converted within 1 minute. The amount of enzyme required for cellobiose and glucose; the enzyme activity of xylanase refers to 1 enzyme activity unit, which means that under specific conditions (35 ° C, other optimal conditions), it can The amount of enzyme required to convert 1 micromol of the substra...

Embodiment 2

[0092] 1. Preparation of functional extractant:

[0093] (1) Water is the extractant, the solid-to-liquid ratio of Auricularia black fungus bran is 1g:1mL, the pH is 4.7, the extraction time is 200s, the extraction voltage is 220v, the extraction temperature is 25°C, and it is extracted quickly in a flash extractor to obtain cellulose-containing Enzyme and xylanase extract, measuring enzyme activity (definition is the same as embodiment 1) is 10IU / g, and 10IU / g is subsequent use;

[0094] (2) 20 parts of physalis stems; 20 parts of red leaves in the mountains; 5 parts of chrysanthemum, 5 parts of dandelion, 5 parts of orange peel, 10 parts of Acanthopanax, 10 parts of black currant, 5 parts of Sophora japonica, 10 parts of Schisandra, 10 parts of tartary buckwheat sticks. Crush, pass through a 20-mesh sieve, mix well and set aside.

[0095] (3) Add 10ml of the enzyme solution extracted in (1) to every 100g of the solid in (2); use 40% ethanol and water- as the solvent, the s...

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Abstract

The invention discloses a culture method capable of increasing the flavonoid content and varieties of auricularia auricula. The culture method is characterized in that functional additives for the culture medium of the auricularia auricula are prepared, the functional additives are added into culture medium to produce the culture medium of the auricularia auricula, and the culture medium is used to culture the auricularia auricula. The culture method includes the steps of firstly, inoculating; secondly, performing spawn running culture, to be more specific, culturing the auricularia auricula strains inoculated in the first step, keeping mycelial growth, and continuing culturing after mycelia overgrow a mycelium bag; thirdly, managing auricularia auricula coming out, to be more specific, punching the bags of the culture medium of the auricularia auricula to allow the mycelia to grow into auricularia auricula sprouts, and continuing culturing; fourthly, harvesting, to be more specific, continuing culturing when the auricularia auricula sprouts in the third step grow into spread auricularia auricula pieces and the edges of the auricularia auricula pieces wrinkle and become soft, and then harvesting to obtain the auricularia auricula. The culture method has the advantages that the culture method is simple, the branches, leaves and stems of waste fungus bran can be recycled favorably, and the flavonoid content and varieties of the cultured auricularia auricula are increased.

Description

technical field [0001] The invention relates to a cultivation method for increasing the content and types of black fungus flavonoids, belonging to the field of cultivation and application of edible fungi. Background technique [0002] Black fungus, also known as black fungus, is a traditional bulk edible fungus species in my country. It is a high-quality nutritious food and health-care food. The sales volume of black food in the market is growing rapidly. [0003] Flavonoids are compounds with a 2-phenylchromone (flavone) structure, which are produced from glucose distribution through the shikimic acid pathway and the acetic acid-malonic acid pathway to generate hydroxycinnamic acid and three molecules of acetic acid, then synthesize chalcone, and then Derived into various flavonoids, it has cardiovascular system activity, antibacterial and antiviral activity, antitumor activity, antioxidative free radical activity, blood pressure lowering, blood lipid lowering, anti-aging, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
CPCA01G18/00C05C11/00C05G3/00C05D3/00C05D3/02C05F5/002C05F11/00
Inventor 柴军红何婷婷钟读波金志民王宝庆
Owner MUDANJIANG NORMAL UNIV
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