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Nanometer graphene oxide-based magnetic resonance imaging contrast agent and preparation method thereof

A magnetic resonance imaging and contrast agent technology, applied in the field of radiodiagnostics, can solve the problems of poor dispersion of physiological solutions, unfavorable promotion and transformation, complicated preparation process, etc., and achieve long circulation time in the body, which is conducive to promotion and productization, biological good compatibility

Inactive Publication Date: 2016-07-27
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current gadolinium ion-based MRI contrast agents using graphene oxide as a carrier still have deficiencies and defects, including: 1) due to the lack of proper functional modification of the graphene oxide used, the dispersion in physiological solutions is poor, and biological Low compatibility, it is difficult to apply in vivo injection, and lacks the possibility of transformation application; 2) Although a certain functional modification is carried out on the graphene oxide used, it still needs to introduce an additional chelating agent, resulting in a complicated preparation process, which is not conducive to popularization and convert

Method used

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  • Nanometer graphene oxide-based magnetic resonance imaging contrast agent and preparation method thereof
  • Nanometer graphene oxide-based magnetic resonance imaging contrast agent and preparation method thereof
  • Nanometer graphene oxide-based magnetic resonance imaging contrast agent and preparation method thereof

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Embodiment 1

[0027] Mix and grind 1g of graphite powder with 60g of NaCl, wash and filter with water to remove NaCl; ultrasonicate the expanded graphite powder with 3:1 concentrated sulfuric acid and concentrated nitric acid for 20h, then measure 23mL of 98%wt concentrated sulfuric acid into the reaction vessel, stir for 8h, and then Then add 5g of potassium permanganate at a rate of 0.1g / min, and stir to react; the mixture is stirred at 40°C for 0.5h, heated to 70°C, stirred for 45min, then added with 3mL of deionized water, stirred at 105°C for 5min; add 40mL Deionized water, stirred at 100°C for 15 minutes; then added 140mL of distilled water, 10mL of 30% hydrogen peroxide solution and stirred for 1h; repeated centrifugation with 5%wt hydrochloric acid and distilled water to obtain nano-graphene oxide, ultrasonically dispersed in water, the color of the solution is brown.

Embodiment 2

[0029] Sonicate 5ml of graphene oxide for 1 hour, add 1.2g of NaOH, 1.0g of chloroacetic acid and sonicate for 3 hours to convert hydroxyl groups into carboxyl groups to generate graphene oxide containing carboxyl groups; then add branched-chain polyethylene glycol (10mg / mL ), corresponding EDC and NHS, generate functionalized nano graphene oxide pGO, add water to dilute, centrifuge and wash, and finally disperse in water to prepare functionalized nano graphene oxide pGO solution;

[0030] After testing, the results show that the pGO aqueous solution is uniform and stable, indicating that it has good dispersion and biocompatibility, and has a long circulation time in the body and a long imaging time window, which is beneficial for in vivo application and imaging diagnosis.

Embodiment 3

[0032] 600mgGdCl 3 Dissolve in 2 mL of deionized water, slowly add 1 ml of pGO solution (1 mg / mL), and stir overnight at room temperature to obtain the mixture; transfer the obtained mixture to a 50KDaMWCO ultrafiltration centrifuge tube for centrifugation and washing, and dilute the centrifuged product to 1 ml to obtain MRI Contrast agent pGO-Gd; especially as a nano-carrier material, pGO has a large specific surface area and a high loading capacity of gadolinium ions, so the relaxation rate is higher, and the MR image quality is higher and clearer;

[0033] In the preparation process, there is no need to introduce chelating agents such as DTPA into the pGO system for further complex modification, and this step is omitted, which makes the preparation of the contrast agent easier and is conducive to popularization and commercialization.

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Abstract

The invention belongs to the technical field of radiodiagnostics, and relates to a nanometer graphene oxide-based magnetic resonance imaging contrast agent and a preparation method and an application thereof, in particular to a magnetic resonance imaging MRI contrast agent pGO-Gd which is directly prepared from gadolinium ions (Gd<3+>) and branched polyethylene glycol functionalized nano graphene oxide (pGO). The contrast agent is prepared by directly chelating O and N atoms in a nano carrier material pGO system and the gadolinium ions; chelating agents of DTPA and the like do not need to be additionally introduced; and the contrast agent has high gadolinium ion load rate. The contrast agent can be applied to in vivo angiography, organ imaging, tumor imaging and lymph node imaging through intravenous injection and skin injection; the contrast agent has the advantages of being simple in preparation, high in magnetic relaxation rate, good in dispersity and compatibility, long in in-vivo circulation time, long in imaging time window, wide in application and the like and is beneficial to conversion and popularization.

Description

technical field [0001] The invention belongs to the technical field of radiodiagnostics, relates to magnetic resonance imaging and a contrast agent thereof, in particular to a magnetic resonance imaging contrast agent based on nanometer graphene oxide and its preparation method and application. Background technique [0002] The prior art discloses that Magnetic Resonance Imaging (MRI) is a non-invasive medical imaging technology with high resolution and can simultaneously obtain anatomical and physiological information, which is beneficial to disease diagnosis in a living state. This technology is widely used in The clinical radiological diagnosis of tumors and nervous system diseases is also an important research direction of molecular imaging. In clinical practice, in order to obtain high-quality MR images, MRI often needs to inject contrast agents to perform enhanced MRI to improve image contrast. Gd-DTPA and other gadolinium ions (Gd 3+ ) chelate is currently a commonl...

Claims

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Application Information

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IPC IPC(8): A61K49/08A61K49/18
CPCA61K49/08A61K49/186
Inventor 王明伟周晓宝曹天野章英剑
Owner FUDAN UNIV SHANGHAI CANCER CENT
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