Preparation method and application of PAAs@MnO(OH)-RGD drug release carrier
A carrier and drug technology, applied in the field of PAAsMnO-RGD drug release carrier, can solve the problems of small contrast agent r1 value, high price change, difficult to form complexes, etc., and achieve the effects of easy degradation, simple preparation process, and reduced usage
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Embodiment 1
[0033] The aqueous solution of PAAsMnO(OH) composite nanospheres was ultrasonically treated for 0.5h, then dropped on the copper grid, dried and then scanned by TEM (see Figure 1(A), Figure 1(B)). The results are shown in Figure 1(A), As shown in Figure 1(B), it can be seen from Figure 1(A) that the PAAsMnO(OH) composite nanomaterial is a nanosphere structure with uniform size and an average diameter of about 80nm; The diameter of the MnO(OH) nanoparticles on the surface is about 2nm, uniform in size and uniform in distribution (pointed by the arrow). From the XRD test results, it can be seen that what is wrapped on the product PAAs is manganese oxyhydroxide nanoparticles (see figure 2).
Embodiment 2
[0035] Add the PAAsMnO(OH) complex and doxorubicin (DOX) to the PBS (10mM, pH=7.4) solution at a mass concentration of 1:1, shake at room temperature for 12 hours in the dark, centrifuge, and wash with PBS (10mM, pH=7.4) Wash the solution, collect the supernatant, calculate the amount of DOX contained therein according to the standard curve of DOX, and further calculate the load of DOX as 0.911mgDOX / 1mgPAAsMnO(OH). Disperse 1mg of the PAAsMnO(OH) complex after adsorbing the drug in 3ml of PBS (10mM, pH=7.4) solution, put it into the dialysis bag, add the dialysis bag to 47ml of PBS solution for dialysis for 48h, every 1h, 2h, 4h, 6h, 8h, 10h, 12h, 14h, 16h, 24h, 36h, 48h, take 3ml solution to measure fluorescence, then add 3ml PBS solution to the original solution, the test results are as follows image 3 Shown: Curve 1 is normal temperature, pH=7.4; Curve 2 is normal temperature, pH=7.4, 2mMDTT; Curve 3 is normal temperature, pH=5; Curve 4 is normal temperature, pH=5, 2mMDTT;...
Embodiment 3
[0037] HepG2 cells were used as target cancer cells to examine the cell biocompatibility of PAAsMnO(OH) nanomaterials: the liver cancer HepG2 cells were seeded in a 96-well plate, the culture medium required by the cells was added, and then cultured in a cell incubator for 24 hours, Take out the culture solution, add the PAAsMnO(OH) nanomaterial prepared in Example 1, and its concentration is divided into PAAsMnO(OH) complexes of 5 μg / ml, 15 μg / ml, 25 μg / ml, 50 μg / ml and 100 μg / ml to carry out In the MTT test, four groups were paralleled for each concentration. After culturing for 24 hours, the absorbance value was read at 490 nm on a microplate reader, and the survival rate of cells in each group was calculated according to the absorbance values of the blank group and the sample group. From Figure 4 It can be seen from the results that the survival rate of cells in each group is above 90%, indicating that the PAAsMnO(OH) nanospheres have good biocompatibility and no obviou...
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