Preparation method and application of paas@mno(oh)-rgd drug release carrier
A carrier and drug technology, applied in the field of PAAs@MnO-RGD drug release carrier, can solve the problems of high valence, small r1 value of contrast agent, and difficulty in forming complexes, and achieves a simple preparation process, easy degradation, and reduced usage. Effect
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Embodiment 1
[0033]The aqueous solution of PAAs@MnO(OH) composite nanospheres was ultrasonically treated for 0.5 h, then dropped on the copper grid, dried and then scanned by TEM (see Figure 1(A), Figure 1(B)). The results are shown in Figure 1(A ), as shown in Figure 1(B), it can be seen from Figure 1(A) that the PAAs@MnO(OH) composite nanomaterial is a nanosphere structure with uniform size and an average diameter of about 80 nm; from Figure 1(B) It can be known that the diameter of the MnO(OH) nanoparticles wrapped on the surface of PAAs is about 2 nm, and the size is uniform and the distribution is uniform (pointed by the arrow). From the XRD test results, it can be seen that what is wrapped on the product PAAs is manganese oxyhydroxide nanoparticles (see figure 2 ).
Embodiment 2
[0035] The PAAs@MnO(OH) complex and doxorubicin (DOX) were added to the PBS (10 mM, pH=7.4) solution at a mass concentration of 1:1, shaken at room temperature for 12 h in the dark, centrifuged, and washed with PBS (10 mM , pH=7.4) solution was washed, the supernatant was collected, the amount of DOX contained in it was calculated according to the standard curve of DOX, and the loading amount of DOX was further calculated as 0.911 mg DOX / 1mgPAAs@MnO(OH). Disperse 1 mg of the PAAs@MnO(OH) complex after adsorbing the drug in 3 ml of PBS (10 mM, pH=7.4) solution, put it into a dialysis bag, add the dialysis bag to 47 ml of PBS solution for dialysis for 48 h, and 1 hour, 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, 12 hours, 14 hours, 16 hours, 24 hours, 36 hours, and 48 hours, take 3 ml of the solution to measure the fluorescence, and add 3 ml of PBS to the original solution solution, the test results are as follows image 3 Shown: Curve 1 is normal temperature, pH=7.4; Curve 2...
Embodiment 3
[0037] HepG2 cells were used as target cancer cells to examine the cell biocompatibility of PAAs@MnO(OH) nanomaterials: the liver cancer HepG2 cells were seeded in a 96-well plate, the culture medium required by the cells was added, and then cultured in a cell incubator for 24 After 1 h, the culture solution was taken out, and the PAAs@MnO(OH) nanomaterial prepared in Example 1 was added at concentrations of 5 μg / ml, 15 μg / ml, 25 μg / ml, 50 μg / ml and 100 μg / ml ml of PAAs@MnO(OH) complex to carry out the MTT test, four parallel groups for each concentration, after incubation for 24 h, then read the absorbance value at 490 nm on a microplate reader, according to the absorbance of the blank group and the sample group Calculate the survival rate of cells in each group. From Figure 4 It can be seen from the results that the survival rate of cells in each group is above 90%, indicating that PAAs@MnO(OH) nanospheres have good biocompatibility and no obvious toxicity, even at high co...
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