UPLC-MS/MS method for measuring antiviral drug residuals in chicken

Abstract

The invention discloses a UPLC-MS / MS method for measuring antiviral drug residuals in chicken. The method comprises the steps that a QuEChERS method is used for sample pretreatment and sample detection analysis, the sample pretreatment comprises sample extraction and purification, the sample extraction is extraction of a combination state sample in a hydrochloric acid-methanol mixture after enzymolysis under the acidic condition, and the sample purification is dispersive solid-phase extraction purification on an extracting solution by using C18 powder and PSA powder adsorbing agents after anhydrous sodium sulfate dehydration. Four types of 12 kinds of antiviral drug residuals with large compound property difference can be measured simultaneously and rapidly by improving the sample extraction and purification method and the chromatography-mass spectrometry conditions, the operation steps are simplified, the detection time is saved, the detection efficiency is improved, and the detection cost and the usage amount of the organic solvent are reduced.

Description

technical field [0001] The invention relates to the fields of analytical chemistry and food safety, in particular to a UPLC-MS / MS method capable of determining antiviral drug residues in chicken. Background technique [0002] Antiviral drugs are a class of drugs used to prevent and treat viral infections. They can inhibit viral replication enzymes in vitro, and prevent virus replication or reproduction in animals or infected cells. They are extremely effective drugs for clinical treatment of viral diseases. With the improvement of the intensive level of livestock and poultry breeding in my country, the occurrence and prevalence of infectious diseases have become an important factor hindering the rapid development of my country's livestock and poultry industry. The rate of animal death due to virus infection is second only to the fatality rate of bacterial infection, accounting for 36.8%. , so antiviral drugs are widely used in the production of livestock and poultry products,...

AI Technical Summary

Problems solved by technology

However, there is no report on simultaneous detection of nucleosides, non-nucleosides, tricyclic amines and non-nucleoside isocyclic amines

In addition, most of the reported methods use liquid-liquid extraction and solid-phase extraction column purification. Among them, liquid-liquid extraction has disadvantages such as limited types and large matrix interference, while solid-phase extraction cartridge (SPE) purification method has high cost and limited scope of application. and other shortcomings

The QuEChERS method is simple, fast, and highly stable, and has been widely used in the detection of pesticide and veterinary drug residues in vegetables, fruits, and aquatic products. However, there is no report on the detection of poultry susceptible to antiviral drugs.

Images