A 3D porous scaffold prepared using pickering high internal phase emulsion as a template

A high-internal phase emulsion and porous scaffold technology, applied in the field of 3D porous scaffold materials, can solve the problems of unfavorable biomedical materials application, large dosage, cytotoxicity, etc., and achieve low toxicity risk, high porosity, and good hydrophilicity Effect

Active Publication Date: 2019-01-25
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the emulsifiers used to stabilize high internal phase emulsions are mostly small molecule surfactants, which are used in a huge amount, and the volume fraction can account for 50% of the external phase. After exceeding a certain concentration, the prepared porous material will cause cytotoxicity. Extremely unfavorable for the application of biomedical materials

Method used

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  • A 3D porous scaffold prepared using pickering high internal phase emulsion as a template
  • A 3D porous scaffold prepared using pickering high internal phase emulsion as a template

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Take 1.25g B-type gelatin and fully dissolve it in 25mL distilled water, then add 25mL desolvating reagent acetone, remove the supernatant, redissolve the white precipitate in 25mL distilled water and adjust its pH value to 12.0, add acetone dropwise until white A precipitate was formed, and then 250 μL of glutaraldehyde solution (25% aqueous solution) was added for cross-linking reaction for 3 hours. Finally, a centrifuge was used to centrifuge the reacted mixed solution at 10000 g for 35 minutes, redissolve the solid in the lower layer of centrifugation in water and slowly evaporate the residual acetone.

[0024] Prepare 3 mL of continuous phase aqueous solution, wherein the concentrations of Pickering stabilizer gelatin nanoparticles, functional monomer acrylamide, initiator ammonium persulfate and crosslinking agent N, N'-methylenebisacrylamide are 5 mg / mL, 2.5mol / L, 3.18mg / mL, 1.77mg / mL. Take 12mL of n-hexane as the dispersed phase, form the high internal phase em...

Embodiment 2

[0026]Take 2.5g of B-type gelatin and fully dissolve it in 50mL of distilled water, then add 50mL of desolvating reagent acetone, remove the supernatant, redissolve the white precipitate in 50mL of distilled water and adjust its pH value to 12.0, add dropwise acetone until white A precipitate was formed, and then 500 μL of glutaraldehyde solution (25% aqueous solution) was added for cross-linking reaction for 6 hours. Finally, a centrifuge was used to centrifuge the reacted mixed solution at 10000 g for 35 minutes, redissolve the solid in the lower layer of centrifugation in water and slowly evaporate the residual acetone.

[0027] Prepare 3 mL of continuous phase aqueous solution, wherein the concentrations of Pickering stabilizer gelatin nanoparticles, functional monomer acrylamide, initiator potassium persulfate and crosslinking agent N, N'-methylenebisacrylamide are 5 mg / mL, 2.5mol / L, 3.18mg / mL, 1.77mg / mL. Take 12mL of toluene as the dispersed phase, form the high interna...

Embodiment 3

[0029] Take 1.25g B-type gelatin and fully dissolve it in 25mL distilled water, then add 25mL desolvating reagent acetone, remove the supernatant, redissolve the white precipitate in 25mL distilled water and adjust its pH value to 12.0, add acetone dropwise until white A precipitate was formed, and then 250 μL of glutaraldehyde solution (25% aqueous solution) was added for cross-linking reaction for 14 hours. Finally, a centrifuge was used to centrifuge the reacted mixed solution at 10000 g for 35 minutes, redissolve the solid in the lower layer of centrifugation in water and slowly evaporate the residual acetone.

[0030] Prepare 3mL continuous phase aqueous solution, wherein the concentrations of Pickering stabilizer gelatin nanoparticles, functional monomer hydroxyethyl acrylate, initiator ammonium persulfate and crosslinking agent N, N′-methylenebisacrylamide are 10 mg / mL, 5.0mol / L, 2.9mg / mL, 11.6mg / mL. Take 12mL p-xylene as the dispersed phase, form the high internal ph...

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Abstract

The invention relates to a 3D porous scaffold material prepared by taking a Pickering high internal phase emulsion as a template and a preparation method of the material. According to the 3D porous scaffold material and the preparation method of the material, gelatin nanoparticles serve as an emulsion stabilizer, water serves as a continuous phase, an organic solvent serves as a dispersion phase, polymerizable functional monomers, an initiator and a crosslinking agent are dissolved in the continuous phase, the oil-in-water type (O / W) Pickering high internal phase emulsion is formed through homogenizing emulsification, and the 3D porous scaffold material is prepared through a polymerization reaction. The prepared 3D porous scaffold material not only has high porosity and a rich multi-layer pore canal structure, but also has a good mechanical property and biocompatibility and can serve as a cell culture scaffold to be widely used in the field of biomedical materials.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a 3D porous scaffold material prepared by using Pickering high internal phase emulsion as a template. Background technique [0002] The most common materials used for cell culture are 2D planar substrates made of polystyrene or glass. However, a major drawback of 2D culture substrates is that they cannot accurately reflect many complex biological responses. To solve these problems, it is very necessary to design some tissue scaffold materials that are very similar to the real growth environment of cells. Therefore, in recent years, many 3D culture substrates that can provide more physiologically relevant growth environments have been developed for in vitro cell culture. [0003] The simple 3D medium model is to culture cells in a scaffold material with good hydrophilicity and biocompatibility. In order to successfully achieve cell adhesion, migration and activation, scaffold...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/28C08F289/00C08F220/56C08F220/58C12N5/00
CPCC08F289/00C08J9/28C08J2351/00C12N5/0062C12N2513/00C12N2533/30C08F220/281
Inventor 谭欢魏涛林炜穆畅道隗晶晶
Owner CHENGDU UNIV
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