Curcumin-loaded collagen-PCL-PVP composite micro-nano fiber membrane and preparation method thereof

Abstract

The invention discloses a curcumin-loaded collagen-PCL-PVP composite micro-nano fiber membrane and a preparation method thereof. The preparation method is characterized by comprising the steps that firstly, dichloromethane and ethyl alcohol which have the certain volume ratio are mixed to be prepared into an electrostatic spinning solvent; secondly, curcumin, PCL and PVP which have the certain mass proportion are mixed to be added into the electrostatic spinning solvent and stirred at room temperature until the mixture is transparent, spinning mother liquid with the concentration of 6%-12% is prepared, the spinning mother liquid is injected into electrostatic spinning equipment to be spun, and then a curcumin-loaded PCL-PVP composite micro-nano fiber membrane is obtained; thirdly, the composite membrane is placed in an ultrasonic generator to be subjected to intermittent ultrasonic treatment; lastly, the composite membrane is steeped into an I type collagen solution and naturally aired, and then the curcumin-loaded collagen-PCL-PVP composite micro-nano fiber membrane is prepared. The material has the good biocompatibility and biodegradability, can promote cell growth, proliferation and adhesion, has the effects of resisting inflammations and oxidization, promoting wound healing and the like and can be used as a tissue engineering scaffold, high-grade dressings, a hemostatic material and the like.

Description

technical field [0001] The invention relates to a curcumin-loaded collagen-PCL-PVP composite micro-nano fiber membrane and a preparation method thereof, belonging to the field of biomedical material preparation. Background technique [0002] Due to the advantages of large specific surface area, high porosity and good physical and mechanical properties, the micro-nanofiber membrane material prepared by electrospinning technology has been widely used in drug slow / controlled release, filter paper, semi-permeable membrane, biosensor, energy source, etc. Storage, cell carrier, environmental engineering, tissue engineering scaffold and other fields. Polycaprolactone (PCL) is a semi-crystalline polymer with excellent biodegradability. At present, the US FDA has approved PCL as a material for surgical sutures, controlled drug release, fracture fixation, and tissue engineering scaffold materials. Substrate. However, studies have shown that PCL has disadvantages such as slow degrada...

AI Technical Summary

Problems solved by technology

In recent years, the preparation of tissue engineering scaffolds by electrospinning collagen has attracted increasing attention. However, it has been found that the three-stranded helical structure of collagen can not be completely preserved in the commonly used electrospinning solvents reported so far, which greatly reduces its superior biological activity. Limiting its medical application, direct introduction of collagen into the micro-nanofibrous scaffold after electrospinning may be the easiest way to maintain its triple helical structure

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