Tissue sealant glue composition, tissue sealant glue, preparation method and application thereof

A technology of sealant and composition, which is applied in the field of biomedicine, can solve the problems of functional effects and use position limitations, and achieve good biocompatibility, high anti-rupture strength, and pain relief effects

Active Publication Date: 2016-11-09
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, GEFOAM's absorbable gelatin (Pharmacia & Upjohn, Kalamazoo, MI), Johnson & Johnson (Shanghai) Medical Equipment Co., Ltd.'s Bioseal and Surgiflo, and fibrin sealants, etc.; although natural biomaterials have t

Method used

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  • Tissue sealant glue composition, tissue sealant glue, preparation method and application thereof
  • Tissue sealant glue composition, tissue sealant glue, preparation method and application thereof
  • Tissue sealant glue composition, tissue sealant glue, preparation method and application thereof

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preparation example Construction

[0039] The present invention provides a tissue sealant composition, a tissue sealant and its preparation method and application. The tissue sealant composition of the present invention mainly includes a first component containing a nucleophile and a second component containing an electrophile , the nucleophilic reagent includes natural substances containing amino groups and / or a first modified product that is modified with a sulfhydryl group on the natural substances containing amino groups, and the natural substances containing amino groups and the first modified products each independently have 10 More than one active group, wherein, the active group is an amino group, a mercapto group; the electrophile includes more than 10 N-succinimides modified on sodium alginate and / or carboxymethyl cellulose The second modified product of the group. Preferably, in the first component, the position where the thiol group is modified is the position of the amino group (eg primary amino gr...

Embodiment 1

[0095]

[0096] 1) Weighing 10mmol (1.6120g) of chitosan with a degree of deacetylation of 87.5%, 15mmol (1.3mL) of mercaptopropionic acid, 20mmol (3.8350g) of soluble carbodiimide, 20mmol (2.3010g) of N-hydroxysuccinimide g), placed in 100 mL of purified water at a temperature of 5° C., and stirred for 8 hours.

[0097] 2) Dialyze the reaction mixture obtained in step 1) in 5 L of normal saline for 12 hours to remove unreacted monomers and small molecular substances, and replace the normal saline every 2 hours.

[0098] 3) Put the mixture obtained by dialysis in step 2) into a freeze dryer for freeze-drying to obtain chitosan modified with sulfhydryl groups, which is the first component.

[0099]

[0100] 1) Weigh 10mmol (2.1610g) of sodium alginate with a viscosity of 500-600mPa·s, 15mmol (2.8760g) of soluble carbodiimide, 15mmol (1.7260g) of N-hydroxysuccinimide, and place in 100mL of Purified water, stirred and reacted for 8 hours.

[0101] 2) Put the reaction mixtur...

Embodiment 2

[0107]

[0108] 1) Weigh 0.4887 poly-L-lysine hydrochloride with a weight-average molecular weight between 3600 and 4300 and a molar percentage of primary amino group content in poly-L-lysine hydrochloride of 35%. g, wherein the molar mass of the primary amino group is 1.5 mmol, and the poly-L-lysine hydrochloride is the first component.

[0109]

[0110] 1) Weigh 15mmol (3.2415g) of sodium alginate with a viscosity of 500-600mPa·s, 15mmol (2.8760g) of soluble carbodiimide, and 15mmol (1.7260g) of N-hydroxysuccinimide. Purified water was stirred and reacted for 10 hours.

[0111] 2) Put the reaction mixture obtained in step 1) into 5 L of normal saline for dialysis for 12 hours to remove unreacted monomers and small molecular substances, and replace the normal saline every 2 hours.

[0112] 3) The mixture obtained by dialysis in step 2) is placed in a lyophilizer for lyophilization to obtain sodium alginate modified with N-succinimide groups, which is the second component...

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Abstract

The invention provides a tissue sealant glue composition, a tissue sealant glue, a preparation method and application thereof. The tissue sealant glue composition comprises a first component and a second component, wherein the first component includes: an amino-containing natural substance and/or a first modified product with the amino-containing natural substance modified by mercapto, and the amino-containing natural substance and the first modified product respectively have more than 10 active groups independently, wherein the active groups are amino and mercapto; and the second component includes: a second modified product with sodium alginate and/or carboxymethyl cellulose modified by more than 10 N-succinimido. The tissue sealant glue has high rupture resistance and low swelling rate, also has good adhesiveness, can rapidly close tissue wound surfaces, effectively plays the role of tissue sealing, prevents tissue fluid, blood or cerebrospinal fluid from leaking, alleviates the suffering of patients, and avoids more risks.

Description

technical field [0001] The invention relates to a tissue sealant composition, a tissue sealant and a preparation method and application thereof, belonging to the technical field of biomedicine. Background technique [0002] Tissue sealant is mainly used in medicine to seal and stop bleeding sites during or after surgery, physically fill defect tissues, prevent tissue viscera from sticking, and prevent interstitial fluid leakage. The main material of tissue sealant can be divided into two categories according to the composition: one is synthetic hydrogel-based materials, such as polyethylene glycol, acrylamide, etc.; the other is natural biological materials, such as gelatin, collagen, chitosan, etc. Sugar, sodium alginate, cellulose, etc. [0003] Synthetic hydrogel materials are divided into two categories: degradable and non-degradable. Among them, the degradable materials are mostly polyethylene glycol, polyethyleneimine and the like. Currently commercially available t...

Claims

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Application Information

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IPC IPC(8): A61L24/08A61L24/04A61L24/00A61L27/20A61L27/18A61L27/58C08G81/00C08B37/08
CPCA61L24/0042A61L24/046A61L24/08A61L27/18A61L27/20A61L27/58A61L2400/04C08B37/003C08G81/00C08L1/286C08L5/04C08L5/08C08L77/04
Inventor 马骋邓坤学袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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