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Beta-receptor agonist substituted template molecularly imprinted polymer microsphere, preparation method and application thereof

A receptor agonist, a technology that replaces templates, applied in microsphere preparation, chemical instruments and methods, microcapsule preparations, etc., can solve problems such as template molecule leakage, and achieve good inclusion and complexation ability and chemical stability.

Inactive Publication Date: 2016-11-09
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, there is also the possibility of leakage of template molecules

Method used

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  • Beta-receptor agonist substituted template molecularly imprinted polymer microsphere, preparation method and application thereof
  • Beta-receptor agonist substituted template molecularly imprinted polymer microsphere, preparation method and application thereof
  • Beta-receptor agonist substituted template molecularly imprinted polymer microsphere, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1, Preparation of β-receptor agonists instead of template molecularly imprinted polymer microspheres (MMIP)

[0054] Dissolve 0.138g of p-hydroxyphenylethylamine and 850mL (0.8024g) of MMA in 15mL of acetonitrile, sonicate for 15min, and place at 25°C for 4h to allow the template molecules to fully interact with the functional monomer, then add 1mL of divinylbenzene (20mmol, 0.919g), AIBN 20mg, sonicated for 10min to make it fully dissolved and mixed, and N was passed into the mixed solution 2 After 2 minutes, it was sealed and polymerized in a water bath at 60°C for 24 hours to obtain a glassy solid polymer (MIP). The polymer is pulverized, the polymer is ground, and then sieved with a 300-mesh sieve to obtain a polymer with a particle size of less than 48 μm; the template molecule is then eluted with a methanol solution with a mass fraction of 5-20% acetic acid, and then methanol is used to Wash until there is no impurity in the washing solution, and then dry...

Embodiment 2

[0057] Example 2. Research on adsorption properties of β-receptor agonists instead of molecularly imprinted polymer microspheres

[0058] (1) Adsorption selectivity of β-agonists instead of molecularly imprinted polymer microspheres

[0059] Accurately prepare 100 ng / mL PBS standard solutions of pH 8.0 of 22 structural analogs, add 10 mg of molecularly imprinted microspheres to absorb for the same time, and perform HPLC-MS / MS detection.

[0060] The adsorption results are shown in Table 3. The results show that the synthetic molecularly imprinted microspheres have the worst adsorption effect on agonists such as clenbuterol, salbutamol, clorprenaline, cimaterol, and mabuterol, and the adsorption rate is less than 35%. . However, the adsorption rates of p-phenylethanolamine A, labeterol, isoxuling and salmeterol can reach more than 99%. The adsorption efficiency of other structural analogs such as ractopamine and bambuterol is relatively low, but they can also be Up to 50% or ...

Embodiment 4

[0073] Embodiment 4, the method application of MMIP to agonist in urine

[0074] 1. Experimental conditions of adsorption and elution time

[0075] (1) Adsorption effect of molecularly imprinted microspheres in urine system

[0076] Take 2mL blank urine sample, place it in a stoppered centrifuge tube, accurately add 100ng / mL agonist and 2mM 0.2mM ammonium acetate solution, 50μL β-glucuronidase / arylsulfatase, 20μL agonist internal standard solution and 10 mg molecularly imprinted microspheres. Vortex to mix for 30s. After reacting for 30 minutes, the adsorption capacity was detected, and the results are shown in Table 2.

[0077] As can be seen from the data in Table 2, although compared with the PBS solution system, the adsorption capacity has declined, but the recovery rate can still reach more than 50%. The results show that the molecular polymer microspheres prepared by the present invention can be applied to urine In the liquid enzymatic hydrolysis system, and the adso...

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Abstract

The invention discloses a beta-receptor agonist substituted template molecularly imprinted polymer microsphere, a preparation method and application thereof. The method comprises the steps of: (1) subjecting a template molecule and methacrylic acid to prepolymerization, with the template molecule being p-hydroxyphenylethanol or p-hydroxyphenethylamine; (2) adding divinyl benzene and azodiisobutyronitrile into the prepolymerized system to carry out polymerization reaction so as to obtain a polymer; and (3) using a methanol solution of acetic acid to wash the polymer, thus obtaining the beta-receptor agonist substituted template molecularly imprinted polymer microsphere. The molecularly imprinted polymer microsphere provided by the invention has the template molecule matching with the steric configuration of the agonist and having holes with multiple action points, and can selectively recognize agonists, thus having better inclusion complexation ability and chemical stability. The invention tests the adsorption properties of the molecularly imprinted polymer microsphere to the beta-receptor agonist, also uses HPLC / MS / MS to conduct adsorption performance detection, and applies the molecularly imprinted polymer microsphere to determination of beta-receptor agonist in urine.

Description

technical field [0001] The invention relates to a β-receptor agonist substituted template molecule imprinted polymer microsphere and its preparation method and application. Background technique [0002] β-receptor agonists (abbreviation, β-agonists) are mainly used in medicine or veterinary clinics to dilate bronchi and increase lung ventilation, and can treat bronchial asthma. β-agonists are widely used in livestock production to promote growth and improve meat quality. In recent years, driven by economic interests, some breeding owners have illegally used β-agonists such as "clenbuterol", which has caused the residues of such drugs in animal foods to seriously exceed the standard, and consumers will have serious side effects after eating such foods. Toxic and side effects, so far there have been many incidents of "clenbuterol" mass poisoning and live pigs being blocked from being supplied to Hong Kong. The occurrence of these incidents has led to the decline of the inter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/06C08F212/36C08J9/28B01J13/02B01J20/26B01J20/30
CPCB01J13/02B01J20/268C08F220/06C08J9/28C08J2333/02C08F212/36
Inventor 索德成王培龙苏晓鸥李阳王瑞国张春艳
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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