Drug injectant for curing dairy cow mastitis and preparation method and application thereof

A technology of breast injections and injections, which can be used in medical formulations, drug combinations, drug delivery, etc. It can solve the problems of decreased immunity and only focus on microbial killing, so as to restore beneficial bacteria and overcome multidrug resistance. , reduce the effect of systemic side effects

Inactive Publication Date: 2017-01-04
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the choice of antibiotic treatment is pure antibacterial thinking, which only pays attention to the killing of microorganisms, but does not pay attention to the other side of the contradiction in the process of mastitis, that is, the inflammatory response of the cow itself and the decline in immunity caused by inflammation. question

Method used

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  • Drug injectant for curing dairy cow mastitis and preparation method and application thereof
  • Drug injectant for curing dairy cow mastitis and preparation method and application thereof
  • Drug injectant for curing dairy cow mastitis and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Preparation of anti-DP-BM-IgY yolk antibody:

[0053] 1. Preparation of soluble bacterial protein antigen complex:

[0054] (1) Select pathogenic Streptococcus agalactiae with specific antigen ( S. agalactiae , CVCC55901, bovine source), Streptococcus dysgalactiae ( S. dysgalactiae , CVCC55935 bovine source), Staphylococcus aureus ( S. aurrus , CVCC 8029 bovine source) and Escherichia coli ( E. coli , O157:K88, CVCC1489 bovine origin). The culture of the main pathogenic bacteria is preferably subcultured on blood agar slant medium, cultured at 37 °C for 24 hours, and there is no miscellaneous bacteria under the staining microscope to obtain pure species. The four main pathogenic bacteria were inoculated in LB broth medium (containing 5-10% calf serum and 1-2% glucose) for expansion culture, and placed in a 37°C constant temperature shaking incubator (150-200 r / min), cultured for 24 h, and the bacterial cells were collected by centrifugation.

[0055] (2) Prepar...

Embodiment 2

[0065] The preparation method of Chinese medicine composition, described Chinese medicine extract is breast injection, specifically:

[0066] Preparation of Chinese medicine extract No. 1:

[0067] 1. Weigh each raw material according to the stated weight (unit: g): 5 Leulu, 5 Dandelion, 5 Viola, 5 Forsythia, 5 Burnet, 5 Trichosanthes, 5 Angelica, and 10 Astragalus.

[0068] 2. Mix all raw materials, stir and pulverize, add 1000mL of water, soak for 0.5 hours, decoct for 2 hours, and filter the decoction; then add 500 mL of water to the filter residue, decoct for the second time for 2 hours, and filter the decoction; Add 500 mL of water to the filter residue, decoct for 2 hours for the third time, and filter the decoction. The above three filtrates were combined, concentrated in a 70°C water bath to 150 mL, and filtered to obtain a concentrated filtrate. Add 95% ethanol to the concentrated filtrate to make the ethanol concentration reach 60%, centrifuge the ethanol precipita...

Embodiment 3

[0082] The preparation method of breast injection drug:

[0083] Take 20 mL of the traditional Chinese medicine extract, add 0.5 mL Tween-80 and 50 mg benzyl alcohol into it, mix well, add water for injection to 90 mL, adjust the pH value to 7.4 with sodium hydroxide, and sterilize at 100°C for 30 min , cooled until the temperature dropped to about 20°C, aseptically added 10 mL of anti-DP-BM-IgY egg yolk antibody, and filled under aseptic conditions to obtain the finished breast injection (preferably 50 mL / bottle). The volume ratio of anti-DP-BM-IgY egg yolk antibody: traditional Chinese medicine extract: water for injection is 10:20:70.

[0084] Further, the extract of the traditional Chinese medicine is preferably No. 4 extract (the solution contains 0.5 g / mL of crude drug), the concentration of the anti-DP-BM-IgY egg yolk antibody protein is 50 mg / mL, and the crude drug content in the final injection drug is 50 mg / mL. 0.1 g / mL.

[0085] Further, in the above-mentioned app...

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Abstract

The invention discloses a drug injectant for curing dairy cow mastitis. The drug injectant comprises a specific anti-DP-BM-IgY egg yolk antibody and an extracting solution of a traditional Chinese medicine composition, and relates to an injectant for breasts. The invention further provides a preparation method and application of the drug injectant. The rug injectant provided by the invention has the beneficial effects as followings: the specific anti-DP-BM-IgY egg yolk antibody can be used for killing main pathogenic bacteria of dairy cow mastitis directionally, plays an obvious curative role on various of mastititides, and has the characteristics of stable quality, strong specificity, zero residue and no resistance to drugs; the traditional Chinese medicine composition also has the efficacies of dissipating blood stasis and relieving pain, repelling poison and dispelling pus, cooling blood and relieving swelling, resisting bacterium and eliminating inflammation, and enhancing immunity; the drug injectant for breasts, obtained through combination, can be directly applied into breasts locally, the targeting effect is better, therefore, bioavailability and drug therapeutic effect are improved, the treatment for diseases is more effective and accurate, the whole body toxic and side effects caused by drugs can be lowered, adverse drug reactions are reduced, the use is convenient, the compliance is good, and the safety in use is enhanced.

Description

technical field [0001] The invention relates to the technical field of veterinary drugs, in particular to a drug injection for treating cow mastitis, a preparation method and application thereof. Background technique [0002] Cow mastitis is an inflammatory response triggered by physical, chemical or microbial stimulation of cow mammary glands, among which the infection of various pathogenic microorganisms is the main cause of cow mastitis. Antibiotics are the first choice for the treatment of mastitis in dairy cows, but there are problems such as insufficient antibacterial spectrum of a single drug, drug resistance, accumulation of drug residues, and intestinal flora imbalance. Fundamentally speaking, the failure of antibiotic treatment is mainly attributed to the pharmacology and pharmacokinetic mechanism of the formation of the special physiological structure of the dairy cow mammary gland. In addition, the choice of antibiotic treatment is pure antibacterial thinking, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61K36/86A61P15/14A61P31/04C07K16/02C07K16/12
CPCA61K39/395A61K9/0019A61K9/0041A61K36/232A61K36/28A61K36/288A61K36/428A61K36/481A61K36/634A61K36/739A61K36/86A61K47/10A61K47/26A61K2236/33A61K2236/331A61K2236/39A61K2236/51A61K2236/53C07K16/02C07K16/1232C07K16/1271C07K16/1275A61K2300/00
Inventor 王玲杨峰苗小楼魏小娟罗永江刘宇郭文柱张彬罗金印杨珍
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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