A method for detecting copper ions in combination with silver nanoprobes and magnetic nanomaterials
A magnetic nanoparticle and magnetic nanotechnology, applied in the field of analytical chemistry, can solve the problems of expensive instruments and time-consuming, and achieve the effects of low cost, less organic solvent consumption, and simple operation
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Embodiment 1
[0022] Embodiment 1: the mensuration of copper content in flue-cured tobacco leaf
[0023] (1) Preparation of silver nanometers: Mix 0.01mol / L silver nitrate 1mL with 0.1mol / L sodium citrate 0.1mL, add deionized water to 10mL, stir at room temperature for 30min, add 0.05mol / L 2,2-thio Diglycolic acid 1mL, continue to stir for 5min, the color of the solution becomes stable yellow and obtained.
[0024] (2) Silanized magnetic nanoparticles are prepared by the following method:
[0025] ① Fe 3 o 4 Synthesis of magnetic nanoparticles: Weigh 2.05g of ferrous ammonium sulfate and 1.41g of ferric chloride and dissolve them in 50mL of deionized water, transfer the mixed solution to a 250mL three-neck flask, mechanically stir and heat in a water bath under the protection of nitrogen, when the reaction solution When heated to 80°C, 5mL of ammonia water (28%, w / v) was added, and the color of the solution changed from brownish yellow to black immediately. After continuous reaction for ...
Embodiment 2
[0029] Embodiment 2: the mensuration of copper content in Burley tobacco leaf
[0030] (1) Preparation of silver nanoparticles: 0.01mol / L silver nitrate 1.5mL mixed with 0.1mol / L sodium citrate 0.5mL, add deionized water to 10mL, stir at room temperature for 20min, add 0.05mol / L 2,2-sulfur Substitute 1.5mL of diglycolic acid, continue to stir for 15min, and the color of the solution becomes stable yellow.
[0031] (2) Silanized magnetic nanoparticles were prepared according to the following method, the same as in Example 1.
[0032] (3) Sample preparation is the same as in Example 1.
[0033] (4) Sample measurement: get the 10mL tobacco leaf sample liquid that step (3) prepares and add the silver nanometer solution that 3mL step (1) prepares, adjust pH to be 8.0 with phosphate buffer solution, stir 30min, form brown-red silver nanometer and metal ion Aggregates, then add 8 mg of silanized ferroferric oxide magnetic nanomaterial prepared in step (2), vortex and mix for 5 min,...
Embodiment 3
[0034] Embodiment 3: Determination of copper content in fresh flue-cured tobacco leaves
[0035](1) Preparation of silver nanoparticles: 0.01mol / L silver nitrate 2mL mixed with 0.1mol / L sodium citrate 0.5mL, add deionized water to 10mL, stir at room temperature for 10min, add 0.05mol / L 2,2-thio Diglycolic acid 2mL, continue to stir for 30min, the color of the solution becomes a stable yellow.
[0036] (2) Silanized magnetic nanoparticles were prepared according to the following method, the same as in Example 1.
[0037] (3) Sample preparation, fresh flue-cured tobacco leaf samples were first dried at 80°C for 1 hour, and then dried at 60°C. Other operations are the same as in Example 1.
[0038] (4) Sample determination: get the 10mL tobacco leaf sample liquid that step (3) prepares and add the silver nanometer solution that 2.5mL step (1) prepares, adjust pH to be 6.0 with phosphate buffer solution, stir 15min, form brown-red silver nanometer and metal Ion aggregates, then...
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