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Bioremediation agent for pesticide residues

A bioremediation agent and pesticide residue technology, which is applied in the field of bioremediation agents for pesticide residues, can solve problems such as restricting development, and achieve the effects of reduced dosage, long storage time, and good stability

Inactive Publication Date: 2017-02-22
FUYANG HUAIBANG MACHINERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are many problems in existing biological agents for degrading organic phosphorus and other pesticide residues, which limit the development of modern

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A bioremediation agent for pesticide residues, said remediation agent is prepared from the following raw materials in parts by weight:

[0031] 10 parts of biosorbent, 20 parts of compound bacterial agent, 2 parts of lignosulfonate, 2 parts of polyvinyl alcohol, 3 parts of fatty alcohol polyoxyethylene ether, 0.5 part of glycerin, and 0.5 part of Tween.

[0032] The biosorbent is prepared by mixing chitosan and diatomaceous earth according to a weight ratio of 1:2;

[0033] The composite bacterial agent is composed of Bacillus amyloliquefaciens, Paracoccus, Trichoderma harzianum, Burkholderia, and Pseudomonas;

[0034] The Bacillus amyloliquefaciens is (Bacillus amyloliquefaciens) ATCC 23843;

[0035] The Paracoccus sp. is CGMCC NO.3639 (CN101899414);

[0036] The Trichoderma harzianum is CGMCC NO.4963 (CN102505010);

[0037] The Burkholderia jiangsuensis CGMCC9028 (CN103952354);

[0038] The Pseudomonas aeruginosa is ATCC 15442.

[0039] The preparation method of ...

Embodiment 2

[0046] A bioremediation agent for pesticide residues, which is prepared from the following raw materials in parts by weight:

[0047] 12 parts of biosorbent, 30 parts of compound bacterial agent, 3 parts of lignosulfonate, 4 parts of polyvinyl alcohol, 5 parts of fatty alcohol polyoxyethylene ether, 0.7 part of glycerin, and 0.7 part of Tween.

[0048] The biosorbent is prepared by mixing chitosan and diatomaceous earth according to a weight ratio of 1:2;

[0049] The composite bacterial agent is composed of Bacillus amyloliquefaciens, Paracoccus, Trichoderma harzianum, Burkholderia, and Pseudomonas; the Bacillus amyloliquefaciens is (Bacillus amyloliquefaciens) ATCC 23843;

[0050] The Paracoccus sp. is CGMCC NO.3639 (CN101899414);

[0051] The Trichoderma harzianum is CGMCC NO.4963 (CN102505010);

[0052] The Burkholderia jiangsuensis CGMCC9028 (CN103952354);

[0053] The Pseudomonas aeruginosa is ATCC 15442.

[0054] The preparation method of the composite bacterial age...

Embodiment 3

[0061] Degradation effect of bioremediation agent of the present invention on methyl parathion, malathion and acephate

[0062] Add 20 mL of inorganic salt medium (g.L -1 , NH 4 NO3 0.5, Na 2 HPO 4 1.19, KH 2 PO 4 0.45, MgSO 4 0.5, deionized water 1000ml, pH6.8), then add methyl parathion, malathion and acephate concentration to 300mg / L respectively, add the bioremediation agent prepared in Example 2.

[0063] Samples were taken regularly, and the concentrations of methyl parathion, malathion and acephate were determined by high performance liquid chromatography.

[0064] HPLC determination of malathion chromatographic conditions: chromatographic column Hypersil C18 column; mobile phase: 70% methanol (containing 2mM ammonium formate+30% water; flow rate: 0.3mL / min; detection wavelength 220nm, column temperature: room temperature; Sample volume: 15 μl.

[0065] HPLC determination of acephate chromatographic conditions: chromatographic column Hypersil C18 column; mobile ...

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Abstract

The invention relates to a bioremediation agent for pesticide residues. The bioremediation agent is prepared from biological absorbent, complex microbial inoculants, lignosulfonate, polyving akohol, fatty alcohol-polyoxyethylene ether, glycerin and polyoxyethylene sorbitan fatty acid ester. According to the bioremediation agent, the degrading capability of degrading organophosphorus pesticides with different degrading characteristics of degrading strains can be fully played by utilizing the combination of bacillus amyloliquefaciens, paracoccus, trichoderma harzianum, burkholderia cenocepacia and pseudomonas of the strains, the requirement of environmental governance of different pollution levels can be satisfied, and application prospects are good.

Description

technical field [0001] The invention relates to the field of bioremediation of pesticides, in particular to a bioremediation agent for pesticide residues. Background technique [0002] Pesticide residue is a general term for the trace pesticide precursors, toxic metabolites, degradation products and impurities that remain in organisms, crops, soil, water, and the atmosphere without being decomposed within a period of time after pesticide use. Some of the pesticides applied to crops are attached to the crops, some are scattered in the soil, air and water environment, and some of the pesticides remaining in the environment will be absorbed by plants. Pesticide residues reach humans and animals directly through plant fruits, water, and air, or pass through the environment and food chain to humans and animals. [0003] At present, the main chemical pesticides used are mainly divided into three categories: insecticides, herbicides and fungicides according to their different func...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40C12N1/20C12N1/14C12R1/07C12R1/01C12R1/885C12R1/38C09K101/00
CPCC09K17/40C09K2101/00C12N1/14C12N1/20
Inventor 叶志坚
Owner FUYANG HUAIBANG MACHINERY