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Phospholipid-glycosaminoglycan biomimetic extracellular matrix nanomembrane and its preparation method and application

A glycosaminoglycan and extracellular matrix technology, which is applied in the field of phospholipid-glycosaminoglycan biomimetic extracellular matrix nanomembrane and its preparation, can solve problems such as unfavorable capillary formation, and achieves the promotion of wound healing and high anticoagulant activity. Effect

Active Publication Date: 2019-02-26
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Porous scaffold-like biomaterials in semi-encapsulated materials can create new transplantation sites for islet transplantation in addition to traditional transplantation sites, but cannot fundamentally solve basic problems such as immune rejection in patients receiving islet transplantation
Wrapping materials include hydrogel, microcapsules, etc., because these materials can completely wrap the islets, so they can solve the immune rejection of the transplanted islets by the patient's body after transplantation, but also due to the complete wrapping of the islets by such materials, It is not conducive to the formation of capillaries, so it is deficient in revascularization after islet transplantation

Method used

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  • Phospholipid-glycosaminoglycan biomimetic extracellular matrix nanomembrane and its preparation method and application
  • Phospholipid-glycosaminoglycan biomimetic extracellular matrix nanomembrane and its preparation method and application
  • Phospholipid-glycosaminoglycan biomimetic extracellular matrix nanomembrane and its preparation method and application

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preparation example Construction

[0030] The invention provides a method for preparing a biomimetic extracellular matrix nano-membrane, comprising the following steps:

[0031] S1: Dissolve glycosaminoglycan in phosphate buffer solution, then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and additives to obtain a mixed solution, and then remove oxygen from the mixed solution Post-activation; wherein, the additive is 1-hydroxybenzotriazole or N-hydroxysuccinimide;

[0032] Preferably, the molar ratio of glycosaminoglycan, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and additive is 1:1:(1~10); Including one or more of heparin, hyaluronic acid and chondroitin sulfate, the relative molecular mass of glycosaminoglycan is 8000-14000; dissolving glycosaminoglycan in phosphate buffer solution specifically includes: dissolving glycosaminoglycan Sugar is added to the phosphate buffer solution to obtain a mixture of glycosaminoglycans and phosphate buffer solution, and then ultrasonicated at 0-37°C for 10-30 minut...

Embodiment 1

[0047] This embodiment provides a biomimetic extracellular matrix nano-membrane and a preparation method thereof, the preparation process comprising:

[0048] S1: Dissolve heparin with a relative molecular mass of 12,000 in a phosphate buffer solution with a pH value of 6.4 to obtain a mixture of heparin and phosphate buffer solution, wherein, in the mixture of heparin and phosphate buffer solution, the mass fraction of heparin 30%. Sonicate the mixture of heparin and phosphate buffer solution at 25°C for 20min with a power of 200W to remove the oxygen in the reaction vessel, then add 1-(3-dimethylaminopropyl)-3-ethyl Carbodiimide and 1-hydroxybenzotriazole to obtain a mixed solution, remove oxygen from the mixed solution, and activate it at 25°C for 30 minutes, wherein heparin, 1-(3-dimethylaminopropyl)-3- The molar ratio of ethylcarbodiimide and 1-hydroxybenzotriazole was 1:1:5.

[0049] S2: Double-distilled water and organic solvents are prepared into a compound emulsion,...

Embodiment 2

[0054] This embodiment provides a biomimetic extracellular matrix nano-membrane and a preparation method thereof, the preparation process comprising:

[0055]S1: Dissolve hyaluronic acid with a relative molecular mass of 8000 in a phosphate buffer solution with a pH value of 6.0 to obtain a mixture of hyaluronic acid and phosphate buffer solution, wherein the mixture of hyaluronic acid and phosphate buffer solution In, the mass fraction of hyaluronic acid is 10%. Sonicate the mixture of hyaluronic acid and phosphate buffer solution at 5°C for 10 minutes, the ultrasonic power is 400W, and remove the oxygen in the reaction vessel, and then add 1-(3-dimethylaminopropyl)-3- Ethylcarbodiimide and 1-hydroxybenzotriazole to obtain a mixed solution, remove oxygen from the mixed solution, and activate it at 5°C for 15 minutes, wherein hyaluronic acid, 1-(3-dimethylaminopropyl )-3-ethylcarbodiimide and 1-hydroxybenzotriazole in a ratio of 1:1:1.

[0056] S2: Double-distilled water and...

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Abstract

The invention relates to a phospholipid-glycosaminoglycan bionic extracellular matrix nanometer membrane and a preparation method and application thereof. The preparation method comprises the following steps of dissolving glycosaminoglycan into a phosphate buffer solution; adding 1-(3-(dimethylamino)propyl)-3-ethyl-carbodiimide hydrochloride and an additive; performing activation after oxygen gas removal, wherein the additive is 1-hydroxybenzotriazole or N-hydroxysuccinimide; adding a mixture of phospholipid and a compound emulsion into an obtained product to take a reaction; and drying the obtained product to obtain the bionic extracellular matrix nanometer membrane. The phospholipid-glycosaminoglycan bionic extracellular matrix nanometer membrane provided by the invention has the advantages that when the extracellular environment can be simulated, the biocompatibility of the bionic extracellular matrix nanometer membrane is improved, so that during the islet cell cluster covering, the activity of the islet cells can be improved; and the insulin releasing function of the islet cells is enhanced. The preparation method of the bionic extracellular matrix nanometer membrane is simple; the reaction conditions are mild; and the operation is simple and convenient.

Description

technical field [0001] The invention relates to the technical field of extracellular matrix materials, in particular to a phospholipid-glycosaminoglycan biomimetic extracellular matrix nano-membrane and its preparation method and application. Background technique [0002] Type I diabetes is an autoimmune disease, which is due to the dysfunction of the body's immune system, which leads to the necrosis of pancreatic β-cells, thereby causing glucose metabolism dysfunction. Although according to International Diabetes Federation (IDF) statistics, type I diabetes patients only account for 10% of total diabetes patients, but type I diabetes occurs earlier, mostly in children and adolescents, so the illness lasts longer, such as improper blood sugar control will lead to Complications in multiple organs including heart, kidney, liver, nerves, and eyes seriously affect the quality of life of patients and cause huge economic burdens on society and families. At present, insulin inject...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/50A61L27/54
CPCA61L27/20A61L27/50A61L27/54A61L2300/412A61L2300/42C08L5/08C08L5/10
Inventor 李宸娄少峰张秀媛孔德领
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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