Method for removing cadmium-containing sewage pollutants
A pollutant and sewage technology, applied in the direction of water pollutants, microbial-based methods, biochemical equipment and methods, etc., can solve problems such as ecological environmental pollution, human physical and mental health hazards, abnormal operation, etc., and achieve good application prospects Effect
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Embodiment 1
[0026] A method for removing pollutants in sewage, which includes the following steps:
[0027] Preparation of the compound bacterial agent: mix the mixed bacterial liquid and the carrier according to a weight ratio of 1:1, stir evenly, then stand for 6 hours, and finally dry at a low temperature at 4°C. After drying, the moisture content is controlled at 6% to obtain; The carrier is obtained by mixing bamboo charcoal, chitosan and diatomaceous earth in a mass ratio of 2:2:1; the above-mentioned mixed bacteria liquid is mixed with the following parts by weight of raw bacteria: 10 parts of Rhodococcus, 9 parts of Thiobacillus denitrificans, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, 5 parts of Bacillus pumilus, 2 parts of Phanerochaete chrysosporium; the concentration of the above-mentioned raw materials is controlled at 1×10 8 Pcs / ml.
[0028] Sewage pretreatment: First, the sewage (NH3-N is 300mg / L, sulfide 80mg / L, phosphorus-containing pollutants is 70mg / L, cadmium...
Embodiment 2
[0031] Use DNAMAN software to design primers, add BamHI and SalI restriction sites respectively, synthesize the nucleotide sequence HP gene according to the amino acid sequence NP_744955.1 full gene synthesis, obtain the target fragment by amplification, and obtain the target gene by PCR amplification HP (At the same time, the corresponding mutation sites 71S / T, 89T / Y, 121T / A, 143D / S, 184S / Q, 239W / G, 246V / P, 269K / N, 304P / S, 353K / E, 369G / D, 372C / S, 422D / R, and 448G / I were introduced into the gene sequence to obtain different mutant genes), the PCR product was double digested with BamHI and SalI, and the PCR product was doubled with BamHI and SalI. The digested cloning expression vector PWB980 is connected, and the successfully verified recombinant plasmid is transformed into the Phanerochaete chrysosporium ATCC24725 to obtain genetically engineered bacteria that degrade cadmium.
Embodiment 3
[0032] Example 3 Verification of sewage treatment effect of Phanerochaete chrysosporium genetically engineered bacteria and other inoculants
[0033] According to the method of Example 1, the corresponding sewage treatment experiment was performed, and the sewage was in the same batch as the sewage of Example 1, and had the same concentration of pollutants. The composition of each component of the bacterial agent is exactly the same as that of Example 1.
[0034] The genetically engineered bacteria at different mutation sites obtained in Example 2 were used to remove cadmium. It was found through experiments that 71S / T, 89T / Y, 121T / A, 143D / S, 184S / Q, 239W / G, 246V / P, 269K / N, 304P / S, 353K / E, 369G / D, 372C / S, 422D / R, 448G / I these mutant bacteria all have significantly enhanced effects compared to the original bacteria. The total treatment time is not more than 4 days, and the results are as follows: the sewage is the same batch of sewage, so as to ensure the same conditions.
[0035] C...
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