Penicillium sclerotiorum and its application

Abstract

The invention provides a strain of Penicillium sclerotiorum and its application and belongs to the technical field of microorganisms. The strain of Penicillium sclerotiorum is preserved in the China center for type culture collection and has an accession number of CCTCC No: M2016543. A green tea filtrate is inoculated with the strain and is fermented so that caffeine is reduced and epigallocatechin gallate effects are reduced. The strain of Penicillium sclerotiorum has strong adaptability and high fermentation state controllability and does not produce toxic and harmful substances. The strain of Penicillium sclerotiorum can also be used to prepare tannase-containing low-caffeine weak-bitter taste green tea beverage raw pulp. The invention provides a novel nutrient-rich low-caffeine content tea beverage retaining the original green tea ingredients. The strain of Penicillium sclerotiorum can greatly reduce the bitter taste of the green tea beverage and provides a new thought for production of a tea beverage.

Description

technical field [0001] The invention belongs to the field of new microorganism varieties and fermentation technology, and in particular relates to a penicillium sclerotinum and application thereof. Background technique [0002] Penicillium sclerotiorum belongs to the genus Penicillium. It is a genus of Ascomycetes with wide distribution. Its colony is cultured on CA at 25°C for 12 days, with a diameter of 25-30 mm, and some isolates can reach 45 mm. There are more or less radial wrinkles; the texture is velvety or banded Flocculate, granular due to many sclerotias; conidia have limited or localized structure, conidia surface is blue-green or dark gray-green, close to bean green, dark ivy green or light gray olive; fungus The filaments often change from white to orange-red or orange-yellow; the exudate is more, reddish, and some isolates are lacking; the reverse side is dark red, brownish red or orange-red; the soluble pigment is similar to the lighter reverse color, and occ...

AI Technical Summary

Problems solved by technology

[0008] Although tannase has a wide range of uses, the market price is expensive due to the complicated production and purification process and the small output, which limits the application of tannase

Tannase is an induced enzyme, which is mainly produced by microbial fermentation. The focus of research at home and abroad is on the screening and mutagenesis of tannase-producing strains, and the production of tannase from environmental samples rich in tannins Isolation and screening of strains, such as tea garden soil, leather factory wastewater, gallnut and other tannin-rich plants, etc., there are few reports on the isolation of endophytic fungi that can produce tannase directly from tea trees

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