Water channel urine protein optical label-free specific detection device and method

Abstract

The invention discloses a water channel urine protein optical label-free specific detection device and method. The device includes optical fiber engraved with tilted fiber grating, a sulfhydryl compound and an AQP2 antibody; the side and the end face of the optical fiber are respectively plated with a layer of metal film with different thicknesses to form an optical fiber sensing probe; the sulfhydryl compound self-assembles into a monomolecular film on the metal film of the optical fiber side, and the AQP2 antibody is fixed on the monomolecular film surface through a catalyst; after fixation of the AQP2 antibody, the optical fiber sensing probe is immersed into a urine stock solution containing AQP2 antigen, and polarized light is input into optical fiber, the characteristic that the metal film surface plasma resonance wave of the optical fiber side is sensitive to the external environment change is utilized to detect the specific binding of AQP2 antigen and AQP2 antibody. The device and the method provided by the invention can realize specific detection of water channel urine protein, replace wavelength demodulation technique with amplitude modulation technique, the detection process is free of label to a detection sample, and also has the advantages of simplicity and speediness, etc.

Description

technical field [0001] The invention relates to an optical label-free specific detection device and method, in particular to an optical label-free specific detection device and method for water channel urine protein, belonging to the field of biomedical optical sensing technology design. Background technique [0002] Water is the source of life. The proportion of water in human cells accounts for about 70% of the total mass. Most of the biochemical reactions related to life activities are carried out in the water environment. Therefore, the occurrence of many diseases is closely related to the destruction of the water balance of the human body. As the main organ of water and fluid metabolism in the human body, the kidney regulates the balance of water and electrolytes in the human body through the production and excretion of urine, and regulates the osmotic pressure of body fluids, the volume of body fluids, and the concentration of electrolytes. Aquaporins (Aquaporins, AQP...

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Problems solved by technology

Although the BCA method has been widely used in the determination of protein concentration, this method measures the total protein content of the solution, rather than the specific detection of water channel urine protein, which is easily interfered by other components in the solution, and also has color development. Slow, easily affected by reducing agent and high concentration of EDTA and other disadvantages

ELISA is slightly expensive because of the large number of detection kits (usually 96-well plates), and is more suitable for large-scale and multi-batch detection activities, which limits the application of ELISA in grassroots work to a certain extent; The influence of ELISA is also greater. If the ambient temperature is too low or too high, the antibody antigen reaction will be weakened or accelerated, making the final result incorrect and difficult to form a standard curve.

The above two methods are applied in the process of drug research and detection. In addition to the use of expensive and large-scale optical instruments (such as 96-well plates), it is also necessary to use statistical methods to simultaneously process a large amount of test data and consume a lot of time. Prepare the sample to be tested, and the sample to be tested must also be placed in the experimental instrument, so these two detection methods cannot meet the in-situ real-time detection of live protein samples

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