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Pleurotus eryngii culture medium capable of realizing high efficiency utilization of cellulose, and preparation method thereof

A technology of cellulose and Pleurotus eryngii, which is applied in the field of Pleurotus eryngii culture material and its preparation with high-efficiency use of cellulose, can solve the problems of increased raw material usage, increased labor, and less use of medium, and achieves the improvement of inorganic elements. Effect

Inactive Publication Date: 2017-03-29
安徽坤霖生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The main problem of the edible fungus culture medium at this stage is that the medium is used less frequently, and many mediums will be discarded after being used twice or even once. The reason is that the nutrients of the medium are difficult to maintain the normal state of the next new fruiting body Growth is mainly due to the lack of nitrogen and carbon sources, and doing so will greatly increase the amount of labor, increase the use of raw materials, and increase the consumption of additional resources. At the same time, it is found that there are still residual There are a lot of nutrients. The main sources of nutrients for the medium used by eukaryotic microorganisms are soluble carbohydrates, sugar, starch and a small amount of low-molecular vitamins. Due to different functions, fungi can rarely use wood chips and cottonseed as raw materials. Husks, rice husks, corn cobs and other raw materials mainly composed of cellulose, resulting in a large amount of unused cellulose components in the culture medium waste, how to use this part of nutrition can greatly increase the use frequency of edible fungus culture medium and reduce Additional investment costs
Cellulase can be hydrolyzed to glucose under their synergistic action, but the significant disadvantages of cellulase are that cellulase has poor stability, short life and low single enzyme activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A Pleurotus eryngii compost with high-efficiency cellulose utilization is characterized in that it is made of the following raw materials in parts by weight (Kg): high-yield cellulase bacterial liquid 14, polyvinyl alcohol 2, 2~4wt% CaCl 2 Solution 9, sodium alginate 0.3, 2~5wt% NaOH solution 95, bamboo shoot shell 24, bran powder 7, soybean peptone, agar powder 1, camphor sawdust 12, cattail grass 50, cassava residue 18, coconut peat 18, coal gangue 25 and the right amount of water;

[0020] The high-yield cellulase bacterial solution is based on 16 g / L of straw material, 6 g / L of wheat flour, 12 g / L of urea, (NH 4 ) 2 SO 4 10 g / L, NH 4 NO 3 6 g / L nutrient solution is used as the medium, heated to gelatinize the wheat flour, and then inoculated with the original species of Trichoderma reesei and Aspergillus niger at a ratio of 5-8:1, and cultured at 30-35°C for 140-150 hours , the rotating speed of the shaking table is 180~200r / min, and the concentration of the de...

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Abstract

The invention discloses a pleurotus eryngii culture medium capable of realizing high efficiency utilization of cellulose, and a preparation method thereof. The pleurotus eryngii culture medium is prepared from, by weight, a high-yield cellulase bacteria solution, polyvinyl alcohol, 2 to 4wt% of a CaCl2 solution, sodium alginate, 2 to 5wt% of a NaOH solution, bamboo shoot shell, wheat bran powder, soy peptone, agar powder, cinnamomum camphora saw dust, scirpoides holoschoenus, cassava slag, coconut residue, coal gangue, and an appropriate amount of water. The pleurotus eryngii culture medium contains a large amount of fiber raw materials such as saw dust and scirpoides holoschoenus, so that decomposition utilization is difficult to realize under general conditions. According to the preparation method, cellulase bacteria is coated with a coupled product of sodium alginate and calcium chloride, and is absorbed by the pleurotus eryngii culture medium, so that the pleurotus eryngii culture medium can be taken as a culture medium for a long term, utilization of cellulose by pleurotus eryngii is realized after decomposition of cellulose; the added ore is subjected to calcining firstly science calcining is beneficial for oxidation of mineral elements, and the mineral elements are decomposed into ions with time passing, so that the content of inorganic elements of the pleurotus eryngii culture medium is increased.

Description

technical field [0001] The invention belongs to the technical field of edible fungus culture medium, and in particular relates to a pleurotus eryngii compost that utilizes cellulose efficiently and a preparation method thereof. Background technique [0002] Edible fungi generally include fruiting bodies and mycelia, which are composed of eukaryotic microorganisms. Under a suitable growth environment such as sufficient nutrition and no restrictions on the individual, the individual will multiply rapidly, resulting in a substantial increase in the number, but when the number of fungi When it is increased to a certain amount, a mycelium composed of fungi will be formed. The dense mycelium is restricted by each other, and the directional formation of the fruiting body primordium is formed by the dense mycelium, which will later produce spores for sexual reproduction. Organs, while the mycelium is composed of tube-shaped cells that absorb nutrients, presenting a fluffy shape with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04
CPCA01G18/00C05C11/00C05G3/00C05D9/00C05F5/002C05F11/00C05F11/08
Inventor 鲁中祝孙苗苗
Owner 安徽坤霖生物科技有限公司