Novel biological seed coating and application thereof

A biological species, bacillus technology, applied in the direction of biocide, application, biocide, etc., can solve the problems of poor product quality, single variety, weak biological seed coating market, etc., to reduce application amount, develop root system, and improve germination. The effect of efficiency and quality

Active Publication Date: 2017-04-26
QINGDAO VLAND BIOTECH GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, my country's biological seed coating agent market is relatively weak, the product quality is poor, and the variety is relatively single. The development of high-quality and safe new biological seed coating agents is an urgent problem to be solved at present.

Method used

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  • Novel biological seed coating and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The preparation method of embodiment 1 microbial mixed fermented liquid

[0025] 1. Activate Paenibacillus polymyxa, Bacillus cereus and Bacillus pumilus respectively; inoculate the activated Paenibacillus polymyxa, Bacillus cereus and Bacillus pumilus into the seeds in sequence according to the ratio of 2-5% Culture medium (LB medium) for mixed culture; under the conditions of 35°C and 120rpm / min, culture on a shaking table for 36-48 hours to obtain the first-grade seed liquid;

[0026] 2. According to the inoculation amount of 10-15%, transfer the primary seed solution to the secondary seed medium in the seed tank (each component and its mass ratio are: starch 1.5-3%, bran powder 1-2 %, yeast extract 0.05-0.3%, peptone 0.5-1%, phosphate rock powder 1-2%, MgSO 4 ·7H 2 O 0.01~0.05%, CaCl 2 0.1~0.4%, (NH4) 2 SO 4 0.01~0.04%, the balance is water, pH 7.0~7.2), cultivated at 28~37℃, with a rotation speed of 100rpm / min, an air flow of 1000L / h, and a tank pressure of ...

Embodiment 2

[0028] The preparation method of embodiment 2 biological seed coating agent

[0029] A biological seed coating agent, the components and contents of which are: the mixed fermentation liquid of Paenibacillus polymyxa, Bacillus cereus and Bacillus pumilus (the number of viable bacteria is about 10 10 CFU / mL, the ratio of the number of viable bacteria of the three bacteria is about 4:3:2), the contents of phytase, sodium alginate, starch, calcium carbonate, Tween 80 and pigment are respectively phytase 2000U / mL, Sodium alginate 9% (w / v), sodium carboxymethylcellulose 3% (w / v), starch 4% (w / v), Tween 80 3% (v / v), calcium carbonate 1%, Pigment 0.3% (w / v);

[0030] Name it No. 1 seed coating agent.

[0031] Weigh each raw material according to the raw materials in the above-mentioned biological seed coating agent, first heat the water to 50°C, stir sodium alginate and starch into the water, dissolve the film-forming solvent completely, then add calcium carbonate, Tween Mix 80 an...

Embodiment 3

[0033]A biological seed coating agent, its components and contents are: a biological seed coating agent, its components and contents are: the mixed fermentation liquid of Paenibacillus polymyxa, Bacillus cereus and Bacillus pumilus (live The number of bacteria is about 10 10 CFU / mL, the ratio of the number of live bacteria of the three kinds of bacteria is about 3:2:2), the contents of phytase, sodium alginate, starch, calcium carbonate, Tween 80 and pigment are respectively phytase 3000U / mL, Sodium alginate 6% (w / v), sodium carboxymethylcellulose 5% (w / v), starch 4.5% (w / v), Tween 80 4% (v / v), calcium carbonate 1.5%, Pigment 0.3% (w / v); name it No. 2 seed coating agent.

[0034] The preparation method is the same as in Example 2.

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Abstract

The invention relates to the technical field of agricultural microorganisms, in particular to a biological seed coating and an application thereof. The biological seed coating can remarkably improve the germination effect of crop seeds of rice, corn and the like due to the synergistic effect of three beneficial microorganisms including paenibacillus polymyxa, bacillus cereus and bacillus pumilus and addition of exogenous phytase, the seed germination rate and the seedling survival rate are generally higher than those of a control group, seedlings have thick stems and developed root system, the plant height is increased by over 23%, the control efficiency of the biological seed coating on underground pest is higher than 95%, the control effect of the biological seed coating on the bakanae disease of rice, corn head smut and basal stalk rot reaches 85% or higher, the application dosage of pesticides in the crop planting process can be effectively reduced, environmental pollution is reduced, and grain safety is guaranteed. The provided biological seed coating finally can effectively increase yield of the crops by improving the seed germination efficiency and quality, so that the yield of the crops such as corn, rice, wheat, soybean and the like is generally increased by 6%-10%, and the effect is remarkable.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a biological seed coating agent containing microorganisms and its application in agricultural production. Background technique [0002] Seed coating technology is a new seed processing technology developed on the basis of traditional seed soaking and seed dressing technology, which makes seed treatment more convenient, safe and effective. A seed coating is a pesticide formulation with film-forming properties used for the treatment of crop or other plant seeds. Biological seed coating agent is a new type of seed coating agent with biological agents as active ingredients, containing microorganisms or their secretions that are beneficial to crops, such as Trichoderma, Rhizobium, and nitrogen-fixing bacteria. [0003] Zhefeng brand ZSB biological seed coating has been patented in 1990. Generally, it can increase the yield of rice by 5%~7%, corn by 8%~10%, wheat ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N63/00A01C1/06A01P21/00A01P7/04A01P3/00
CPCA01C1/06A01N63/00A01N63/10A01N2300/00
Inventor 宗睿李佩佩刘鲁民刘金
Owner QINGDAO VLAND BIOTECH GRP
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