Non-viral gene transfection vector material based on cationic helical peptide

A technology of gene transfection carrier and cation, which is applied in the field of non-viral gene transfection carrier materials and its preparation, can solve the problems of large acidity changes, low efficiency, and many steps, and achieve the effect of low cytotoxicity and high transfection efficiency
CN106589355AActive Publication Date: 2017-04-26GUANGZHOU MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU MEDICAL UNIV
Publication Date
2017-04-26

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Abstract

The invention belongs to the field of biomedical macromolecular materials and discloses a non-viral gene transfection vector material based on cationic helical peptide, and a preparation method and application thereof. The structure of the vector material is shown by general formulas (1) to (6) in the description, wherein R1 is C1-6 alkyl, benzyl, methoxypolyethylene glycol, polyethylene glycol or polypropylene oxide, R2 is C1-3 alkyl, benzyl or phenethyl, R3 is methyl or ethyl, R4 is hydroxy, and A is H, C1-3 alkyl, alkoxy, halogen or nitryl; in the general formulas (1) to (4), x represents polymerization degree of polypeptide and is not less than 5; in the general formulas (5) and (6), x represents the ratio of side chain repeating units, with 0<x<1. The cationic helical polypeptide main chain is of Alpha-helical conformation and effectively forms complex micelles with DNA and siRNA, and the material has good transfection efficiency, low cell toxicity and applicable to the transfer of pDNA and siRNA.
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Description

technical field

[0001] The invention belongs to the field of biomedical polymer materials, and in particular relates to a non-viral gene transfection carrier material based on a cationic helical polypeptide and its preparation method and application. Background technique

[0002] Gene therapy is one of the most promising technologies emerging today for the treatment of tumors and various immune diseases. It regards genes as a "drug" to treat diseases, which is an advanced treatment method. This therapy can treat a variety of serious diseases, including various congenital genetic diseases and acquired diseases, such as tumors, acquired immunodeficiency syndrome, cardiovascular diseases, cystic fibrosis, etc., among which the treatment of tumors is current research hotspot. There are two ways of gene therapy, one is to replace the disordered gene in the cell with exogenous gene, or to correct the problem gene with exogenous gene, which belongs to DNA therapy; the other is to...

Claims

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