Method for extracting fucoidan through biological enzymolysis process

A technology of fucoidan and bio-enzyme, which is applied in the direction of drug combination, blood disease, extracellular fluid disease, etc., can solve the problems of not completely destroying the cell wall structure, affecting the reuse of products, and low content of effective substances, and achieves strong decolorization ability , simplified extraction steps, strong adsorption effect

Inactive Publication Date: 2017-06-13
安徽天安生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] (1) The above methods cannot completely destroy the cell wall structure, but fully release the fucoidan in the cell wall, and the extraction rate is low;
[0010] (2) The appearance of the product is light yellow, not beautiful enough, and there is still an algae smell, which affects the reuse of the product;
[0011] (3) Us

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] A method for extracting fucoidan by biological enzymatic hydrolysis provided in this example comprises the following steps:

[0070] Step (1): Kelp pretreatment, cleaning kelp, cutting into small pieces, grinding into slurry;

[0071] Step (2): Add water 7 times the weight of the slurry into the slurry, stir evenly, soak for 12 hours, then transfer the slurry into the enzymatic hydrolysis container;

[0072] Step (3): Add acid cellulase and hemicellulase into the enzymolysis container, the amount of acid cellulase and hemicellulase is respectively 11% of the weight of the slurry after adding water in step (1), stir evenly, The pH is controlled between 4.5 and the temperature is controlled between 55°C, reacted for 8 hours, and cooled to obtain product 1. Since the pH is controlled at 6.5 and the temperature is controlled at 45°C, the activity of acid cellulase and hemicellulase The highest, so the enzymatic hydrolysis effect is also the best, which can enzymatically hy...

Embodiment 2

[0081] A method for extracting fucoidan by biological enzymatic hydrolysis provided in this example comprises the following steps:

[0082] Step (1): Kelp pretreatment, cleaning kelp, cutting into small pieces, grinding into slurry;

[0083] Step (2): Add water 5 times the weight of the slurry into the slurry, stir evenly, soak for 12 hours, then transfer the slurry into the enzymatic hydrolysis container;

[0084] Step (3): Add acid cellulase and hemicellulase into the enzymolysis container, the amount of acid cellulase and hemicellulase is respectively 9% of the weight of the slurry after adding water in step (1), stir evenly, The pH is controlled at 6.5, the temperature is controlled at 45°C, reacted for 12 hours, and cooled to obtain product 1;

[0085] Step (4): Add alkaline pectinase to Product 1, the amount of alkaline pectinase added is 6% of the weight of the slurry after adding water in step (1), stir evenly, control the pH at 10.0, and control the temperature at 40...

Embodiment 3

[0093] A method for extracting fucoidan by biological enzymatic hydrolysis provided in this example comprises the following steps:

[0094] Step (1): Kelp pretreatment, cleaning kelp, cutting into small pieces, grinding into slurry;

[0095] Step (2): Add water 5-7 times the weight of the slurry into the slurry, stir evenly, soak for 12-36 hours, then transfer the slurry into the enzymatic hydrolysis container;

[0096] Step (3): Add acid cellulase and hemicellulase into the enzymolysis container, the amount of acid cellulase and hemicellulase is respectively 9-11% of the weight of the slurry after adding water in step (1), stir Uniform, PH control between 4.5-6.5, temperature control between 45-55 ℃, react for 8-12 hours, cool to get product 1;

[0097] Step (4): Add alkaline pectinase to product 1, the amount of alkaline pectinase added is 6-10% of the weight of the slurry after adding water in step (1), stir evenly, and control the pH between 8.0-10.0 , the temperature is...

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PUM

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Abstract

The invention belongs to the technical field of fucoidan extraction and provides a method for extracting fucoidan through a biological enzymolysis process. The method comprises the following steps: grinding kelp into slurry; soaking; adding acidic cellulase and hemicellulase; adding alkaline pectinase; adding protease; adding sodium chloride and water and reacting, thereby acquiring sodium alginate jelly; adding modified attapulgite; centrifuging by using a centrifugal machine; and adding ethyl alcohol and dewatering, thereby acquiring solid-phase fucoidan. According to the method for extracting fucoidan provided by the invention, the biological enzymolysis process is adopted; on the basis of the structure and ingredients of kelp cytoderm, a defined amount of related enzyme is added in stages, so that the cytoderm is thoroughly split; the fucoidan therein is fully released, so that the extraction rate of the fucoidan is increased; the modified attapulgite is added for decolorizing and removing fishy smell in an extraction process, so that the appearance of the product is cleaner; and besides, the extraction steps are simplified, the use of the chemical raw materials is reduced and the purpose of environmental protection is achieved.

Description

technical field [0001] The invention belongs to the technical field of extracting seaweed polysaccharides, and in particular relates to a method for extracting fucoidan by a biological enzymatic hydrolysis method. Background technique [0002] Seaweed is algae that grows in the sea. Generally speaking, algae can be divided into four types: green algae, brown algae, red algae and blue algae. They are cryptogamous plants in the plant kingdom. Algae include several different types that produce energy through photosynthesis biology. Seaweed is rich in nutrients and functional ingredients. Alginate or seaweed polysaccharide extracted from seaweed has special emulsification, gelling properties, medicinal value, immunity enhancement and anticancer activity, so it is gradually used in industry and pharmaceutical industry middle. [0003] Seaweed polysaccharides have the effects of inhibiting viruses, lowering cholesterol, lowering blood sugar and blood lipids. Among them, fucoida...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06A61P7/02A61P31/12A61P35/00A61P43/00
CPCC08B37/0063
Inventor 何声武程贤防
Owner 安徽天安生物科技股份有限公司
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