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Method for producing L-lactic acid from lignocellulose through fermentation

A technology of lignocellulose and lactic acid, applied in the field of fermentation, can solve the problems of inconsistent fermentation temperature, polluted environment process, long fermentation cycle, etc., and achieves the effects of strong continuity, less environmental pollution, and short fermentation cycle

Inactive Publication Date: 2017-06-13
JIANGSU DIYIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN 102174600A discloses a method for producing L-lactic acid by continuous fermentation, and CN 102134581 A discloses a method for lactic acid bacteria fermentation, in which both crop waste or plant straw are pretreated with acid and alkali, and lactic acid bacteria are used for continuous fermentation at 40°C. Fermentation or simultaneous saccharification and fermentation, although the patent shortens the fermentation cycle by optimizing the process, but the acid-base pretreatment still has the problems of polluting the environment and complex processes in the later stage
At the same time, the fermentation temperature is inconsistent with the optimum cellulase enzymatic hydrolysis temperature (48°C-50°C), so there is a problem of low enzymatic hydrolysis efficiency in the simultaneous saccharification process
[0007] Therefore, the method of utilizing lignocellulose to produce L-lactic acid also has problems such as pretreatment, environmental pollution, long fermentation cycle and high production cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of Bacillus coagulans fermented seed liquid: Streak inoculation of Bacillus coagulans onto the culture medium plate, place in a 50°C incubator for 18 hours, pick multiple rings with an inoculation needle and inoculate it in 7L liquid medium, and incubate at a temperature of Cultivate for a certain period of time in a 10L mechanically stirred fermenter at 50°C with a rotation speed of 180rpm and an aeration rate of 1vvm. Seed medium composition: glucose 20g / L, yeast powder 10 g / L, sodium chloride 2 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 0.2 g / L, manganese sulfate monohydrate 0.05 g / L L, ferrous sulfate heptahydrate 0.01 g / L, calcium hydroxide was used to adjust pH during seed cultivation;

[0025] (2) Coarsely crush the corn stalks to about 5mm, mix them with tap water at a mass ratio of 1:1, stir evenly, add them to the steam explosion tank, heat them to 200°C with saturated steam, keep them for 10 minutes, release the ...

Embodiment 2

[0028] (1) Preparation of Bacillus coagulans fermenter seed liquid: Streak inoculation of Bacillus coagulans onto the culture medium plate, place in a 50°C incubator for 18 hours, use an inoculation needle to pick up multiple rings and inoculate it in 7L liquid medium, at temperature The temperature is 50 ℃, the rotation speed is 180rpm, and the 10L mechanical stirring fermenter is cultivated for a certain period of time, and the ventilation rate is 1vvm. Seed medium composition: glucose 20g / L, yeast powder 10 g / L, sodium chloride 2 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 0.2 g / L, manganese sulfate monohydrate 0.05g / L L, ferrous sulfate heptahydrate 0.01 g / L, calcium hydroxide was used as pH neutralizer during seed cultivation;

[0029] (2) Coarsely crush the rape straw to 5mm, mix it with tap water at a mass ratio of 1:1, stir evenly, add it to the steam explosion tank, heat it to 200°C with saturated steam, keep it for 10 minutes, release th...

Embodiment 3

[0032] (1) Preparation of Bacillus coagulans fermenter seed liquid: Streak inoculation of Bacillus coagulans onto the culture medium plate, place in a 50°C incubator for 18 hours, use an inoculation needle to pick up multiple rings and inoculate it in 7L liquid medium, at temperature The temperature is 50 ℃, the rotation speed is 180rpm, and the 10L mechanical stirring fermenter is cultivated for a certain period of time, and the ventilation rate is 1vvm. Seed medium composition: glucose 20g / L, yeast powder 10 g / L, sodium chloride 2 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 0.2 g / L, manganese sulfate monohydrate 0.05g / L L, ferrous sulfate heptahydrate 0.01 g / L, calcium hydroxide was used as pH neutralizer during seed cultivation;

[0033] (2) Coarsely crush the Miscanthus stem to 5mm, mix it with tap water at a mass ratio of 1:1, stir evenly, add it to the steam explosion tank, heat it to 200°C with saturated steam, keep it for 10 minutes, relea...

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Abstract

The invention discloses a method for producing L-lactic acid from lignocellulose through fermentation. The method comprises the following steps that 1, bacillus coagulans fermentation seed culturing is conducted; 2, crop straw or energy plants are pretreated through a steam explosion method; 3, L-lactic acid is produced by conducting saccharification and fermentation simultaneously, wherein fermentation medium ingredients are added into a fermentation tank, a bacillus coagulans fermentation seed solution cultured in the step 1 and the crop straw or energy plants pretreated through steam explosion in the step 2 are added, simultaneous saccharification and fermentation are conducted for 48 h-72 h, samples are taken regularly to measure the concentration of lactic acid in the fermentation liquid. The method has the comprehensive advantages of being simple in process, short in fermentation period, high in lactic acid optical purity, easy to purity, free of pollution in the whole production process and the like, the production cost is effective controlled, and industrialization is convenient to achieve.

Description

technical field [0001] The invention belongs to the technical field of fermentation, in particular to a method for producing L-lactic acid by fermenting lignocellulose. Background technique [0002] Lactic acid has two stereoisomers, namely L-(+) lactic acid and D-(-) lactic acid. As an important biochemical raw material, L-lactic acid is widely used in the fields of food, medicine, health care, modern agriculture and daily chemical industry. In recent years, with the in-depth research on new biological materials, bio-based polymers, especially polylactic acid formed by polymerization of L-lactic acid as a monomer, have attracted much attention. As a biodegradable material, polylactic acid is the most promising environment-friendly bioplastic, which has the potential to solve the "white pollution" problem caused by petrochemical plastics. [0003] The production methods of lactic acid include chemical method and microbial fermentation method. Among them, the chemical metho...

Claims

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Application Information

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IPC IPC(8): C12P7/56C12P19/14C12P19/02C12R1/07
Inventor 陈璐王骞
Owner JIANGSU DIYIN BIOTECH
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