Fast extraction and LC-MS-MS detection method of benzimidazole and thiazole type residual medicine in aquatic product

Abstract

The invention discloses a fast extraction and LC-MS-MS detection method of benzimidazole and thiazole type residual medicine in an aquatic product. The method comprises the following steps that (1) muscle samples of the aquatic product are weighed; internal standard deuteratedalbendazole, deuterated mebendazole, magnesium sulfate, sodium chloride and acetonitrile are added for vortex extraction; (2) C18 powder and neutral alumina are added into extraction liquid; after swirling, nitrogen gas is used for blow-drying; filtering is performed after redissolution; (3) filter liquid is subjected to LC-MS-MS quantitative measurement; an internal standard method is used for calculating the residual quantity of levamisol, amino-mebendazole, hydroxyl-mebendazole, mebendazole, albendazole sulfoxide, albendazole sulphone, albendazole amino sulfone and albendazole. The method has the advantages that the sample treatment is simple, convenient and fast; meanwhile, the pH value of a liquid chromatography water phase and a cleaning agent is preferentially selected; the residual quantity of various benzimidazole and thiazole type medicines in the aquatic product can be simultaneously measured; the sensitivity is high; the repeatability is good; the accuracy is high; the lowest quantification of the medicine detection can reach 1mu g / kg.

Description

technical field [0001] The invention belongs to the field of quality and safety of aquatic products, and in particular relates to a rapid extraction method of benzimidazole and thiazole residues in aquatic products and a LC-MS-MS detection method. Background technique [0002] With the expansion of aquaculture scale, large-scale parasitic diseases continue to occur, and a large number of insecticides are applied to aquaculture. Significant drug residues were caused by off-label use and non-compliance with withdrawal periods. Most antiparasitic drugs have certain toxicity. For example, clinical trials have shown that most benzimidazole drugs have teratogenic and mutagenic effects. Serious adverse reactions have also occurred during the use of such drugs, such as anaphylactic shock, severe measles and encephalitis syndrome, which have attracted widespread attention from various countries. In 2004, my country's Adverse Drug Reaction Monitoring Center reported for the first ti...

AI Technical Summary

Problems solved by technology

At present, the extraction methods used in these detection methods still remain in liquid-liquid extraction and solid-phase extraction, which require a lot of manpower and a long time for sample processing, and a large amount of organic reagents are used in the processing methods, resulting in a large amount of waste. liquid and waste gas, causing pollution to the environment

Images