Soybean protein polypeptide and applications thereof

Abstract

The invention discloses a soybean protein polypeptide. The soybean protein polypeptide preparation method comprises: an alkali dissolving step, a first enzymatic hydrolysis step, a second enzymatic hydrolysis step, and an enzyme inactivation step, wherein a microwave assisted technology is respectively used in each step. According to the present invention, the preparation method is simple, the process is less, the yield and the purity of the obtained soybean protein polypeptide are high, and the obtained soybean protein polypeptide has high biological activity and can effectively improve the physical condition of the drinker. The invention further discloses a soybean protein polypeptide wine, which has fragrant and mellow taste, can make human body highly absorb the peptide, and has effects of nutrition supplementing, human immunity improving, blood circulation promoting, and good health effect.

Description

Technical field

[0001] The invention relates to a soybean protein, in particular to a soybean protein polypeptide and an application thereof. Background technique

[0002] Protein is the most important component in nature and the basic element that constitutes organic organisms. All the life activities of all animals and plants are closely related to protein. At the same time, protein is the first nutrient required by the human body, accounting for 45% of the total body dry matter and 70% of the total muscle. The main components of human cells, tissues, and organs are proteins. The body's metabolism, disease resistance, immunity, body fluid balance, and genetic information transmission are all closely related to protein. Without protein, there is no life, so protein is also called the "first nutrient" or "life element" of human beings.

[0003] Soybeans are the yellow seeds of the leguminous plant soybean and are the "king of beans". It contains about 40% protein, which is com...

Examples

preparation example Construction

[0040] A soybean protein polypeptide. The preparation method of soybean protein polypeptide includes:

[0041] Alkali dissolving step: take soybean powder, add purified water to prepare a slurry, adjust the pH of the slurry to 8-9, then place it in a microwave extraction device and heat for 12-18min, control the temperature at 52-58℃, turn off the microwave and keep warm 0.8-1.2h, filter, take the filtrate to obtain protein liquid;

[0042] The first enzymolysis step: lower the temperature of the protein solution to room temperature, adjust the pH to 7-8, then add the first protease, stir well to obtain the first mixed solution; then place the first mixed solution in the microwave extraction device Medium heating for 12-18min, control the temperature at 52-58℃, and keep the pH value of the first mixed solution above 6.5 to obtain the first enzymolysis solution;

[0043] The second enzymolysis step: lower the temperature of the first enzymolysis solution to room temperature, adjust t...

Embodiment 1

[0070] A soy protein polypeptide wine prepared according to the following method:

[0071] A. Preparation of soybean protein peptide

[0072] Alkali dissolving step: take the soybean powder with a particle size of 230-400 mesh, add purified water according to the solid-liquid ratio of 1:15 to prepare a slurry, and add 2% of the mass of soybean powder xanthan gum, and then adjust the pH of the slurry with NaOH solution To 8.5, then place it in a microwave extraction device with a frequency of 2500MHz and a power of 20KW and heat it for 15 minutes to bring the temperature to 55°C, turn off the microwave and keep it warm for 1 hour, filter, and take the filtrate to obtain a protein solution;

[0073] The first enzymolysis step: lower the temperature of the protein solution to room temperature, adjust the pH to 7.5, and then add serine protease and pectinase. The addition amount of serine protease and pectinase are respectively 3% and 3% of the protein content in the protein solution. 1...

Embodiment 2

[0084] The difference between Embodiment 2 and Embodiment 1 is:

[0085] Alkali dissolving step: take the soybean powder with a particle size of 230-400 mesh, add purified water according to the solid-liquid ratio of 1:12 to prepare a slurry, and add locust bean gum of 5% by mass of the soybean powder, followed by Na 2 CO 3 The solution adjusts the pH value of the slurry to 8, and then places it in a microwave extraction device with a frequency of 2450MHz and a power of 19KW and heats it for 18 minutes to bring the temperature to 52°C, turn off the microwave and keep it for 1.2h, filter, and take the filtrate to obtain a protein solution;

[0086] The first enzymolysis step: lower the temperature of the protein solution to room temperature, adjust the pH to 8, and then add serine protease and pectinase. The added amount of serine protease and pectinase is 2% of the protein content in the protein solution. Stir thoroughly to obtain the first mixed liquid; then place the first mixed l...

Technical field

[0001] The invention relates to a soybean protein, in particular to a soybean protein polypeptide and an application thereof. Background technique

[0002] Protein is the most important component in nature and the basic element that constitutes organic organisms. All the life activities of all animals and plants are closely related to protein. At the same time, protein is the first nutrient required by the human body, accounting for 45% of the total body dry matter and 70% of the total muscle. The main components of human cells, tissues, and organs are proteins. The body's metabolism, disease resistance, immunity, body fluid balance, and genetic information transmission are all closely related to protein. Without protein, there is no life, so protein is also called the "first nutrient" or "life element" of human beings.

[0003] Soybeans are the yellow seeds of the leguminous plant soybean and are the "king of beans". It contains about 40% protein, which is com...

Examples

preparation example Construction

[0040] A soybean protein polypeptide. The preparation method of soybean protein polypeptide includes:

[0041] Alkali dissolving step: take soybean powder, add purified water to prepare a slurry, adjust the pH of the slurry to 8-9, then place it in a microwave extraction device and heat for 12-18min, control the temperature at 52-58℃, turn off the microwave and keep warm 0.8-1.2h, filter, take the filtrate to obtain protein liquid;

[0042] The first enzymolysis step: lower the temperature of the protein solution to room temperature, adjust the pH to 7-8, then add the first protease, stir well to obtain the first mixed solution; then place the first mixed solution in the microwave extraction device Medium heating for 12-18min, control the temperature at 52-58℃, and keep the pH value of the first mixed solution above 6.5 to obtain the first enzymolysis solution;

[0043] The second enzymolysis step: lower the temperature of the first enzymolysis solution to room temperature, adjust t...

Embodiment 1

[0070] A soy protein polypeptide wine prepared according to the following method:

[0071] A. Preparation of soybean protein peptide

[0072] Alkali dissolving step: take the soybean powder with a particle size of 230-400 mesh, add purified water according to the solid-liquid ratio of 1:15 to prepare a slurry, and add 2% of the mass of soybean powder xanthan gum, and then adjust the pH of the slurry with NaOH solution To 8.5, then place it in a microwave extraction device with a frequency of 2500MHz and a power of 20KW and heat it for 15 minutes to bring the temperature to 55°C, turn off the microwave and keep it warm for 1 hour, filter, and take the filtrate to obtain a protein solution;

[0073] The first enzymolysis step: lower the temperature of the protein solution to room temperature, adjust the pH to 7.5, and then add serine protease and pectinase. The addition amount of serine protease and pectinase are respectively 3% and 3% of the protein content in the protein solution. 1...

Embodiment 2

[0084] The difference between Embodiment 2 and Embodiment 1 is:

[0085] Alkali dissolving step: take the soybean powder with a particle size of 230-400 mesh, add purified water according to the solid-liquid ratio of 1:12 to prepare a slurry, and add locust bean gum of 5% by mass of the soybean powder, followed by Na 2 CO 3 The solution adjusts the pH value of the slurry to 8, and then places it in a microwave extraction device with a frequency of 2450MHz and a power of 19KW and heats it for 18 minutes to bring the temperature to 52°C, turn off the microwave and keep it for 1.2h, filter, and take the filtrate to obtain a protein solution;

[0086] The first enzymolysis step: lower the temperature of the protein solution to room temperature, adjust the pH to 8, and then add serine protease and pectinase. The added amount of serine protease and pectinase is 2% of the protein content in the protein solution. Stir thoroughly to obtain the first mixed liquid; then place the first mixed l...

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