Utilizing amphoteric surfactant to promote enzymatic hydrolysis of lignocellulose and cooling method for recovering cellulase
A technology of lignocellulosic enzymes and surfactants, applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of easy inactivation of cellulase and affect the activity of enzyme systems, and achieve simple operation and avoid high temperature loss The effect of liveliness and low energy consumption
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Embodiment 1
[0030] Such as figure 2As shown, take 100 parts by mass of microcrystalline cellulose, add it to 5000 parts by mass of acetic acid-sodium acetate buffer solution with a pH of 4.8 and an ionic strength of 50 mmol / L, add 50 parts by mass of SB3-16, and then add 10 FPU / g to The cellulase of the microcrystalline cellulose mass meter was reacted at 50°C for 48 hours. After the reaction, centrifuged to obtain the enzymolysis liquid, lowered the temperature of the enzymolysis liquid to 0°C, and centrifuged when a large amount of precipitation appeared in the solution , then add the obtained solid to the same sample as the initial enzymatic hydrolysis conditions (substrate and buffer) and enzymatically hydrolyze it again for 48h (without supplementing cellulase and amphoteric surfactant), and measure twice by biosensor analyzer The enzymatically hydrolyzed glucose content, the statistical results are shown in Table 1.
Embodiment 2
[0032] Such as figure 2 As shown, take 100 parts by mass of Eucalyptus-DA, add it to 5000 parts by mass of citric acid-sodium citrate buffer solution with a pH of 5.0 and an ionic strength of 25mmol / L, add 50 parts by mass of SB3-16, and then add 5FPU / g Cellulase, counted as dextran in the substrate, reacted at 50°C for 48 hours. After the reaction, centrifuged to obtain the enzymolysis liquid, lower the temperature of the enzymolysis liquid to 0°C, and when a large amount of precipitation appeared in the solution Carry out centrifugation, then add the obtained solid to the same sample as the initial enzymatic hydrolysis conditions (substrate and buffer) and enzymatic hydrolyze again for 48h (without supplementing cellulase and amphoteric surfactant), and pass through the biosensing analyzer The glucose content of the two enzymatic hydrolysis was measured, and the statistical results are shown in Table 1.
Embodiment 3
[0034] Such as figure 2 As shown, take 250 parts by mass of pine wood-SPORL, add it to 5000 parts by mass of a phosphate buffer solution with a pH of 5.5 and an ionic strength of 100 mmol / L, and add 100 parts by mass of C 12 PPS, then add 10FPU / g cellulase based on glucan in the substrate, react at 50°C for 72h, after the reaction, centrifuge to obtain the enzymolysis liquid, reduce the temperature of the enzymolysis liquid to 0°C, and wait When there is a large amount of precipitation in the solution, perform centrifugation, and then add the obtained solid to the same sample as the initial enzymatic hydrolysis conditions (substrate and buffer) for another 72 hours of enzymatic hydrolysis (without supplementing cellulase and amphoteric surfactant), The glucose content of the two enzymatic hydrolysis was measured by a biosensor analyzer, and the statistical results are shown in Table 1.
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