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Composite biological preservative and preparation method thereof

A technology of biological preservatives and vitamins, applied in food ingredients as antimicrobial preservation, food science, food preservation, etc., can solve the problems of limited antibacterial range and low inhibition rate, and achieve a wide range of antibacterial and applicable scope Broad, small environmental pH and temperature dependent effects

Inactive Publication Date: 2017-09-29
苏州凌科特新材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current biological preservatives mainly include nisin and natamycin. Although both have good antibacterial effects, their antibacterial range has certain limitations. For Gram-negative bacteria, mold and yeast The inhibition rate of bacteria and other substances is low, and it has a certain dependence on the environmental pH and temperature.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A preparation method of a composite biological preservative, comprising the following preparation steps:

[0021] Step 1. Weigh each raw material by weight: 20 parts of N-benzyloxycarbonyl-D-aspartic acid, 3 parts of nisin, 2 parts of L-polylysine, 1 part of vitamin B12, niacin 1.2 parts of chromium, 2 parts of sodium glycine fumarate, 0.6 parts of clove basil oil, 2 parts of puffball powder, 0.4 parts of soil tuckahoe powder, 0.6 parts of nard pine powder, 0.2 parts of silicon dioxide, 2.4 parts of organic acid, 0.5 parts of borneol part, 1.8 parts of disodium EDTA, and 44 parts of distilled water; wherein, the organic acid is a mixture of fruit acid and glycine with a mass ratio of 1:2; the molecular weight of the L-polylysine is 1-100,000;

[0022] Step 2. Mix puffball powder, soil tuckahoe powder, nard pine powder, silicon dioxide and clove basil oil evenly, heat to 50°C, stir for 15 minutes, and cool to room temperature;

[0023] Step 3. Mix and stir sodium glycin...

Embodiment 2

[0026] A preparation method of a composite biological preservative, comprising the following preparation steps:

[0027] Step 1. Weigh each raw material by weight: 38 parts of N-benzyloxycarbonyl-D-aspartic acid, 15 parts of nisin, 11 parts of L-polylysine, 7 parts of vitamin B12, niacin 5 parts of chromium, 7 parts of sodium glycine fumarate, 1.9 parts of clove basil oil, 4.2 parts of puffball powder, 1.3 parts of soil tuckahoe powder, 2.2 parts of nard pine powder, 0.9 parts of silicon dioxide, 5.6 parts of organic acid, 2 borneol 3.1 parts, 3.1 parts of disodium EDTA, 60 parts of distilled water; wherein, the organic acid is fruit acid; the molecular weight of the L-polylysine is 1-100,000;

[0028] Step 2. Mix puffball powder, soil tuckahoe powder, nard pine powder, silicon dioxide and clove basil oil evenly, heat to 70°C, stir for 40 minutes, and cool to room temperature;

[0029] Step 3. Mix and stir sodium glycine fumarate, vitamin B12, L-polylysine, N-benzyloxycarbony...

Embodiment 3

[0032] A preparation method of a composite biological preservative, comprising the following preparation steps:

[0033] Step 1. Weigh each raw material by weight: 22 parts of N-benzyloxycarbonyl-D-aspartic acid, 3.5 parts of nisin, 2.6 parts of L-polylysine, 1.5 parts of vitamin B12, niacin Chromium 1.7 parts, sodium glycine fumarate 2.2 parts, clove basil oil 0.8 parts, puffball powder 2.3 parts, soil tuckahoe powder 0.6 parts, nard pine powder 0.9 parts, silicon dioxide 0.3 parts, organic acid 2.6 parts, borneol 0.6 parts 1.9 parts, 1.9 parts of disodium EDTA, 46 parts of distilled water; wherein, the organic acid is one or more of citric acid, lactic acid, fruit acid and glycine; the molecular weight of the L-polylysine is 1- 100,000;

[0034] Step 2. Mix puffball powder, soil tuckahoe powder, nard pine powder, silicon dioxide and clove basil oil evenly, heat to 60°C, stir for 25 minutes, and cool to room temperature;

[0035] Step 3. Mix and stir sodium glycine fumarate...

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PUM

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Abstract

The invention discloses a composite biological preservative and a preparation method thereof. The composite biological preservative is prepared from N-benzyloxycarbonyl-D-aspartic acid, nisin, L-polylysine, vitamin B12, chromium nicotinate, sodium glycine fumarate, Oleum Ocimi gratissimi, powder of Lycoperdon, powder of Smilax glabra, powder of Nardostachyos roots and rhizomes, silica powder, organic acid, borneol, disodium ethylene diamine tetraacetate and distilled water. The composite biological preservative provided by the invention has a wide bacteriostatic scope, exerts good bacteriostatic effect on Gram-negative bacteria, saccharomycetes, Escherichia coli and Staphylococcus aureus, and is low in dependence on the pH value and temperature of environment and wide in an application scope.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a composite biological preservative and a preparation method thereof. Background technique [0002] Preservatives are one of the key additives of food and feed products, which directly affect the shelf life and quality of food and feed products, and reduce the damage caused by various bacteria or toxins to products during production, processing, transportation and storage. Different degrees of pollution can lead to economic losses. Research on preservatives has also become one of the research and development and application hotspots in the field of food and feed products. Traditional preservatives are chemical preservatives, and common ones include sodium benzoate, potassium sorbate, sodium dehydroacetate, calcium propionate, and diacetic acid. Sodium, Sodium Lactate, Propyl Paraben and Hydrogen Peroxide etc. Some preservatives will be decomposed by microorganisms. For ...

Claims

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Application Information

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IPC IPC(8): A23L3/3463A23L3/3553A23L3/358A23L3/34
CPCA23L3/34A23L3/34635A23L3/3553A23L3/358A23V2002/00A23V2200/10A23V2250/0622A23V2250/706A23V2250/032A23V2250/042Y02A40/90
Inventor 李子寅
Owner 苏州凌科特新材料有限公司
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