Edible fungus dreg decay promoting complex microbial agent and preparation method thereof
A compound bacterial agent and edible fungus technology, applied in the field of agricultural biology, can solve the problems of limited species and quantity, poor fermentation effect, odor and fertilizer efficiency, etc.
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Embodiment 1
[0022] Embodiment 1: Preparation of edible fungus slag rot-promoting compound bacterial agent
[0023] Experimental Materials:
[0024] (1) Bacteria strains used in the preparation of bacterial agents
[0025] Aspergillus oryzae p1, Sporosporium thermophila, Trichoderma viride, Trichoderma reesei and Aspergillus oryzae p2 were isolated and screened from the compost of edible fungus slag with cottonseed hulls as the main material.
[0026] (2) Medium
[0027] PDA medium: potato 200g, glucose 20g, agar 15g, distilled water 1000mL.
[0028] Solid expansion medium: solid carrier 77% (mass percentage wood chips: diatomaceous earth = 8:2), glucose 2%, urea 0.5%, lime 0.5%, distilled water 20%
[0029] experimental method:
[0030] (1) Single-bacteria plate culture: Aseptically inoculate Aspergillus oryzae p1, Dispospora thermophila, Trichoderma viride, Trichoderma reesei and Aspergillus oryzae p2 on PDA medium respectively, in a constant temperature incubator at 30°C Culture fo...
Embodiment 2
[0034] Embodiment 2: the preparation of edible fungus slag rot-promoting composite bacterial agent
[0035] Experimental Materials:
[0036] (1) Bacteria strains used in the preparation of bacterial agents
[0037] Aspergillus oryzae p1, Sporosporium thermophila, Trichoderma viride, Trichoderma reesei and Aspergillus oryzae p2 were isolated and screened from the compost of edible fungus slag with cottonseed hulls as the main material.
[0038] (2) Medium
[0039] PDA medium: potato 200g, glucose 20g, agar 15g, distilled water 1000mL.
[0040] Solid expansion medium: solid carrier 77% (mass percentage wood chips: diatomaceous earth = 8:2), glucose 2%, urea 0.5%, lime 0.5%, distilled water 20%
[0041] experimental method:
[0042] (1) Single-bacteria plate culture: Aseptically inoculate Aspergillus oryzae p1, Dispospora thermophila, Trichoderma viride, Trichoderma reesei and Aspergillus oryzae p2 on PDA medium respectively, in a constant temperature incubator at 30°C Cultu...
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