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Edible fungus dreg decay promoting complex microbial agent and preparation method thereof

A compound bacterial agent and edible fungus technology, applied in the field of agricultural biology, can solve the problems of limited species and quantity, poor fermentation effect, odor and fertilizer efficiency, etc.

Inactive Publication Date: 2017-10-03
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural composting only relies on the indigenous microorganisms in the composting raw materials. Due to the limited types and quantities of indigenous microorganisms, it is difficult to rapidly reproduce and degrade the cellulose and crude protein in the edible fungus residue in the compost, and there is a fermentation time. Long, poor fermentation effect, odor and low fertilizer efficiency, poor degree of harmlessness, greatly limited production and application
This problem can be solved by using external bacteria agents, but the organic waste decomposing agents currently on the market are mainly aimed at household garbage, straw-like agricultural waste, poultry manure, etc. However, there is a lack of high-efficiency decomposing bacteria agents for edible fungus residues in the cultivation substrate of cottonseed hulls.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Preparation of edible fungus slag rot-promoting compound bacterial agent

[0023] Experimental Materials:

[0024] (1) Bacteria strains used in the preparation of bacterial agents

[0025] Aspergillus oryzae p1, Sporosporium thermophila, Trichoderma viride, Trichoderma reesei and Aspergillus oryzae p2 were isolated and screened from the compost of edible fungus slag with cottonseed hulls as the main material.

[0026] (2) Medium

[0027] PDA medium: potato 200g, glucose 20g, agar 15g, distilled water 1000mL.

[0028] Solid expansion medium: solid carrier 77% (mass percentage wood chips: diatomaceous earth = 8:2), glucose 2%, urea 0.5%, lime 0.5%, distilled water 20%

[0029] experimental method:

[0030] (1) Single-bacteria plate culture: Aseptically inoculate Aspergillus oryzae p1, Dispospora thermophila, Trichoderma viride, Trichoderma reesei and Aspergillus oryzae p2 on PDA medium respectively, in a constant temperature incubator at 30°C Culture fo...

Embodiment 2

[0034] Embodiment 2: the preparation of edible fungus slag rot-promoting composite bacterial agent

[0035] Experimental Materials:

[0036] (1) Bacteria strains used in the preparation of bacterial agents

[0037] Aspergillus oryzae p1, Sporosporium thermophila, Trichoderma viride, Trichoderma reesei and Aspergillus oryzae p2 were isolated and screened from the compost of edible fungus slag with cottonseed hulls as the main material.

[0038] (2) Medium

[0039] PDA medium: potato 200g, glucose 20g, agar 15g, distilled water 1000mL.

[0040] Solid expansion medium: solid carrier 77% (mass percentage wood chips: diatomaceous earth = 8:2), glucose 2%, urea 0.5%, lime 0.5%, distilled water 20%

[0041] experimental method:

[0042] (1) Single-bacteria plate culture: Aseptically inoculate Aspergillus oryzae p1, Dispospora thermophila, Trichoderma viride, Trichoderma reesei and Aspergillus oryzae p2 on PDA medium respectively, in a constant temperature incubator at 30°C Cultu...

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PUM

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Abstract

The invention relates to the technical field of agricultural biotechnology and particularly relates to an edible fungus dreg decay promoting complex microbial agent and a preparation method thereof. The microbial agent comprises Aspergillus oryzae p1, Sporotrichum thermophile, Trichoderma viride, Trichoderma reesei and Aspergillus oryzae p2. Trichoderma viride, Trichoderma reesei and Sporotrichum thermophile have strong cellulase activity and can degrade a large amount of cellulose in edible fungus dreg into small molecule monosaccharides. Aspergillus oryzae has strong protease activity and can hydrolyze proteins in edible fungus slag into small molecule peptone, polypeptide and a variety of amino acids. At the beginning of composting, the fungus dreg is degraded by Trichoderma viride, Trichoderma reesei and Aspergillus oryzae and the compost is fast heated. In the high temperature stage, Sporotrichum thermophile accelerates the degradation of cellulose in composting materials. The synergistic effects of all strains are realized so that a complex and stable symbiotic relationship is formed. The complex microbial agent can keep the fermentation temperature of the compost at 55 to 65 DEG C, and the composting cycle is reduced to 10 to 15 days.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to an edible fungus slag decay-promoting composite bacterial agent and a preparation method thereof. Background technique [0002] Since the reform and opening up, my country's edible fungus industry has experienced rapid development for more than 30 years. The annual output has exceeded 28 million tons, the output value has exceeded 200 billion yuan, and the indirect output value has exceeded 700 billion yuan, accounting for 5% of the total output value of the planting industry. The number of employees is about 20 million. It is the fifth largest crop after grain, oil, fruit and vegetables. my country is the world's largest producer and consumer of edible fungi, and its annual output accounts for about three-quarters of the world's edible fungus output. However, the prosperity of the edible fungi industry has also brought certain environmental problems, such as producing a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F5/00C05F17/00C12R1/69C12R1/645C12R1/885
CPCC05F5/00C05F17/00C12N1/14C05F11/08Y02A40/20Y02W30/40
Inventor 戴璐李峻志祁鹏张黎光李安利
Owner MICROBIOLOGY INST OF SHAANXI
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