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Heterotrophic nitrification-aerobic denitrification arthrobacterium and application thereof

A technology of aerobic denitrification and heterotrophic nitrification, which can be used in bacteria, chemical instruments and methods, biochemical equipment and methods, etc., and can solve the problems of staying and few reports.

Active Publication Date: 2017-10-13
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, heterotrophic nitrification-aerobic denitrification has mainly remained at the stage of laboratory exploration, and there are not many reports on the practice of nitrogen treatment in wastewater

Method used

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  • Heterotrophic nitrification-aerobic denitrification arthrobacterium and application thereof
  • Heterotrophic nitrification-aerobic denitrification arthrobacterium and application thereof
  • Heterotrophic nitrification-aerobic denitrification arthrobacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Isolation of Heterotrophic Nitrifying-Aerobic Denitrifying Arthrobacter WZUF01

[0038] (1) Primary screening

[0039] 5ml of activated sludge taken from the aeration tank of Wenzhou Dongpian Sewage Treatment Plant was inoculated into 100ml enrichment medium (sodium succinate 5g, NaNO 3 1g, KH 2 PO 4 1g, K 2 HPO 4 1g, (NH 4 ) 2 SO 4 0.25g, MgSO 4 ·7H 2 O 0.2g, yeast powder 1g, H 2 O 1000ml, pH 7.0~7.2) in a 250ml Erlenmeyer flask, shake culture at 25°C, 150rpm for 3 days; take 10ml enrichment medium and transfer to fresh enrichment medium and culture under the same conditions, repeat 5 times; the enriched culture solution was properly diluted and inoculated on bromothymol blue (Bromothymol Blue) plate (sodium succinate 5g, NaNO 3 1g, KH 2 PO 4 1g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 1g, CaCl 2 2H 2 O 0.2g, FeSO 4 ·7H 2 O 0.05g, asparagine 1g, bromothymol blue 1ml (prepared with 1% ethanol), agar 18g, H 2 O 1000ml, pH 7.0~7.2), cultured a...

Embodiment 2

[0056] Example 2: Identification of Heterotrophic Nitrifying-Aerobic Denitrifying Arthrobacter WZUF01

[0057] The young cells of the strain WZUF01 are irregular rods, often arranged in a V shape, and the rods break into small balls during the growth process, with obvious rod and ball cycle changes, G + (See Figure 4 ); aerobic, catalase positive.

[0058] Amplify 16SrDNA using bacterial genomic DNA as a template, using a pair of universal primers: upstream primer (P1): 5'-AGAGTTTGATCCTGGTCAGAACGAACGCT-3', downstream primer (P6): 5'-TACGGCTACCTTGTTACGACTTCACCCC-3', PCR, PCR product The purification and sequencing were completed by the China Industrial Microbiology Culture Collection Management Center, and the sequencing results were compared and analyzed by GenBank Blast.

[0059] The 16SrDNA of the WZUF01 strain consists of 1594bp bases, as shown in SEQ ID NO.1; through GenBankBlast comparison analysis, it has high homology with the 16SrDNA sequence of Arthrobacter in GenB...

Embodiment 3

[0061] Example 3: Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification Arthrobacter WZUF01

[0062] The preserved heterotrophic nitrifying-aerobic denitrifying Arthrobacter WZUF01 (1.5mL frozen tube thawed bacterial liquid) was inoculated in 100ml LB medium (peptone 10g, yeast powder 5g, NaCL 5g, H 2 O 1000ml) in a 250ml Erlenmeyer flask, cultivated at 25°C and 150r / min for 12h, centrifuged at 5000r / min for 15min, collected the thalli, washed 3 times with sterile distilled water, and prepared OD 600 =0.9~1.0 bacterial suspension.

[0063] Take 5ml and inoculate it into a 250ml Erlenmeyer flask containing 100ml medium, shake and culture at a certain temperature and rotation speed (basic temperature and rotation speed are 25°C and 150rpm, respectively) for 24h, then centrifuge at 5000r / min for 10min to obtain the supernatant , and the supernatant was subjected to relevant assay analysis.

[0064] The basic medium consists of: sodium succinate...

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Abstract

The invention discloses a heterotrophic nitrification-aerobic denitrification arthrobacterium and application in denitrification of wastewater. The bacteria strain is Arthrobacter sp. WZUF01, and is preserved into Common Microorganism Center of China General Microbiological Culture Collection Center; the registration number in the collection center is CGMCC NO.14012. The bacteria strain has the advantages that the nitrogen in the nitrogen-containing wastewater is removed, the pH (potential of hydrogen) and temperature application ranges are broader, the carbon and nitrogen mass ratio is lower, and the higher content of dissolved oxygen can be endured; the high concentration of ammonia can be endured, the wastewater with NH4<+>-N concentration more than 300mg / L from a culture farm after anaerobic digestion can be treated, the treatment result meets the pollutant emission standard of livestock and poultry culture industry (GB18596-2001), and the final product is nitrogen; the gathering ability is good; the Arthrobacter sp. WZUF01 has greater application potential in the actual nitrogen removal of the wastewater.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, in particular to a heterotrophic nitrifying-aerobic denitrifying Arthrobacter and its application in removing nitrogen from sewage. Background technique [0002] The pollution caused by nitrogen to the environment has become increasingly prominent in recent years, and its harmfulness has been increasingly recognized and valued by people. For example, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen may be transformed into carcinogenic, mutagenic and teratogenic nitrosamines; another example is the inflow of nitrogen into water bodies to cause eutrophication of water bodies, causing deterioration of water quality and even lake degradation. Biological nitrogen removal has the advantages of good treatment effect, stable and reliable treatment process, and convenient operation and management, so it has been widely used. Traditional biological denitrification is accomplish...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/06C02F101/38
CPCC02F3/34C02F2101/38C12N1/205C12R2001/06
Inventor 周茂洪赵肖为
Owner WENZHOU UNIVERSITY
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