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Preparation method of compound microorganism bacterium agent for sludge treatment

A compound microbial agent and sludge treatment technology, applied in the direction of microbial-based methods, biochemical equipment and methods, biological sludge treatment, etc., can solve the problem of inactivation of bacterial flora physiological activity, cumbersome and complicated preparation process, complex flora Large inoculum volume and other problems, to achieve the effect of improving application activity, increasing the decomposition rate of organic matter, and improving domestication performance

Inactive Publication Date: 2017-10-13
PULIZI ENVIRONMENT TECH SUZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to achieve a good number of flora in the process of activating the strains, each flora is generally cultivated in the way of one bacterium and one culture. With the enrichment of the flora, the preparation process is cumbersome and complicated. The large inoculum amount of bacteria is not conducive to the improvement of economic benefits; and in the composting fermentation process, high temperature is conducive to shortening the composting cycle, accelerating the decomposition of organic matter, and reducing water content, but as the temperature increases, the physiological activity of the bacteria is affected. Inhibition or even inactivation, the unsuitability of the bacteria to high temperature is not conducive to the composting, if the high temperature resistance of the bacteria can be improved, it is of great significance for reducing the addition of bacteria and improving the efficiency of composting

Method used

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  • Preparation method of compound microorganism bacterium agent for sludge treatment
  • Preparation method of compound microorganism bacterium agent for sludge treatment
  • Preparation method of compound microorganism bacterium agent for sludge treatment

Examples

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Embodiment 1

[0038] A method for preparing a composite microbial agent for sludge treatment, comprising mixing Escherichia coli, bacillus, sulfur oxidizing bacteria, actinomycetes, nitrifying bacteria and lactic acid bacteria to prepare a composite microbial agent;

[0039] Its preparation method comprises the following steps:

[0040] The first step: the cultivation of Escherichia coli and actinomycetes:

[0041] Weigh 15g of nutrient broth culture based on 500ml of distilled water, add 0.1g of tricyclodecanylamine solid, after shaking and stirring, put it into a vertical pressure steam sterilizer for sterilization and disinfection, turn on the ultra-clean workbench and turn on the ultraviolet light to sterilize for 10 minutes, After autoclaving, take out the culture medium and place it in the ultra-clean workbench, wait for the temperature of the culture medium to drop to about 60°C, inoculate E. Bacteria, then put into a biochemical incubator and adjust the temperature of the incubator...

Embodiment 2

[0049] A preparation method for sludge treatment composite microbial bacterial agent, comprising the following steps:

[0050] The first step: the cultivation of Escherichia coli and actinomycetes:

[0051] Weigh 15g of nutrient broth culture based on 500ml of distilled water, add 1g of tricyclodecanylamine solid, after shaking and stirring, put it into a vertical pressure steam sterilizer for sterilization, turn on the ultra-clean workbench and turn on the ultraviolet light to sterilize for 20min, wait for After high-pressure sterilization, take out the culture medium and place it in the ultra-clean workbench, wait for the temperature of the culture medium to drop to about 70°C, inoculate E. coli into the culture medium under aseptic conditions, inoculate the E. , then put it into a biochemical incubator and adjust the temperature of the incubator to 38°C, and incubate for 10 hours;

[0052] The second step: the cultivation of bacillus and lactic acid bacteria:

[0053] Wei...

Embodiment 3

[0059] A preparation method for sludge treatment composite microbial bacterial agent, comprising the following steps:

[0060] The first step: the cultivation of Escherichia coli and actinomycetes:

[0061] Weigh 15g of nutrient broth culture based on 500ml of distilled water, add 0.1g of tricyclodecanylamine solid, after shaking and stirring, put it into a vertical pressure steam sterilizer for sterilization and disinfection, turn on the ultra-clean workbench and turn on the ultraviolet light for 20 minutes of sterilization, After autoclaving, take out the culture medium and place it in the ultra-clean workbench, wait for the temperature of the culture medium to drop to about 60°C, inoculate E. Bacteria, then put into a biochemical incubator and adjust the temperature of the incubator to 38°C, and incubate for 8 hours;

[0062] The second step: the cultivation of bacillus and lactic acid bacteria:

[0063] Weigh 15g of nutrient broth based on 500ml of distilled water, shake...

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Abstract

The invention discloses a preparation method of a compound microorganism bacterium agent for sludge treatment. The compound microorganism bacterium agent is prepared by mixing escherichia coli, bacillus, sulfur-oxidizing bacteria, actinomyces, nitrifying bacteria and lactic acid bacteria; meanwhile, the invention provides the preparation method of the compound microorganism bacterium agent for the sludge treatment. According to the preparation method disclosed by the invention, the adaptation activity of a compound bacterium colony to temperature can be improved through adding adamantanamine and diethylmalonylurea; the adding amount of the bacterium colony can be reduced and the composting efficiency also can be improved; the compound microorganism bacterium agent disclosed by the invention can be used for effectively killing harmful microorganisms and pathogens in sludge through composting fermentation; meanwhile, a large amount of evaporation of moisture is accelerated and the problem of treating residual sludge in a wastewater treatment system is solved so that the dehydration efficiency of the sludge is improved.

Description

Technical field: [0001] The invention relates to the technical field of sludge treatment, in particular to a preparation method of a sludge treatment bacterial agent. Background technique: [0002] With the development of my country's social economy and urbanization, the number of urban sewage treatment plants is increasing. By the end of 2014, my country's annual treatment capacity was 48 billion cubic meters of sewage, and the corresponding annual sludge production was 35 million tons (80% moisture content). However, for a long time There is a phenomenon of "heavy water and light mud" in our country, which leads to the fact that most of the sludge produced in sewage treatment has not been properly treated and disposed of, resulting in new environmental problems. [0003] The principles of sludge treatment are reduction, recycling, harmlessness, and temperature. The sludge with 80% water content is bulky and contains a lot of water, organic matter and a certain amount of to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C02F11/14C12R1/19C12R1/07C12R1/01
CPCC12N1/20C02F11/02C02F11/14
Inventor 张飞周东
Owner PULIZI ENVIRONMENT TECH SUZHOU CO LTD
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