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A method for quantitative analysis of ibuprofen in a biological sample by utilizing a cyclodextrin-modified holographic sensor

A holographic sensor and quantitative analysis technology, which is applied in the direction of material analysis by observing the influence of chemical indicators, analysis by making materials undergo chemical reactions, color/spectral characteristic measurement, etc., can solve the problems that cannot meet the needs of research analysis and clinical testing , reliance on precision and expensive instruments, insufficient detection sensitivity, etc., to achieve the effect of simple and flexible method, flexible and diverse device structure, and high recovery rate of standard addition

Active Publication Date: 2017-11-10
LINYI UNIVERSITY
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  • Application Information

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Problems solved by technology

[0003] At present, the main detection methods of traditional ibuprofen that have been reported include solid phase extraction and liquid phase extraction, etc. These traditional detection methods have disadvantages such as insufficient detection sensitivity, complicated and time-consuming operation, relying on sophisticated and expensive instruments, and requiring professional personnel to operate. , often cannot meet the requirements of research analysis and clinical testing

Method used

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  • A method for quantitative analysis of ibuprofen in a biological sample by utilizing a cyclodextrin-modified holographic sensor
  • A method for quantitative analysis of ibuprofen in a biological sample by utilizing a cyclodextrin-modified holographic sensor
  • A method for quantitative analysis of ibuprofen in a biological sample by utilizing a cyclodextrin-modified holographic sensor

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preparation example Construction

[0045] 3.2 Preparation of spiked plasma samples

[0046] Slowly thaw the purchased plasma (H4522-100mL, Sigma-Aldrich) in an ice bath, filter it through a 0.22 μm filter membrane, dilute it with a phosphate buffer solution with an ionic strength of 100 mM and a pH of 7.5, and vortex After vortexing, centrifuge at 3600 rcf at 4°C for 10 min, and carefully remove the supernatant as a plasma sample.

[0047] In order to ensure that the pH and ionic strength of the sample solution are the same, the ibuprofen standard solution (ionic strength is 100 mM and pH is 7.5) of the same volume and different mass concentrations is added to the plasma sample to obtain plasma samples with different spiked concentrations.

[0048] Plasma samples and an equal proportion mixture of phosphate buffer solution with an ionic strength of 100 mM and a pH of 7.5 were used as a blank sample solution.

[0049] 3.3 Preparation of spiked urine samples

[0050] Since the pH of human urine is 5.5 to 7, the...

Embodiment

[0054] 1. Preparation of glass slides modified with silane reagents

[0055] Spread the glass slide on the tray, pour an appropriate amount of 2% (v / v) silane reagent acetone solution, shake the tray evenly, make the slide glass completely immersed in the acetone solution, tilt slightly, suck out the acetone, and put the tray Store in a dark room at room temperature. After overnight, wash with deionized water, wipe dry, and put in a small glass slide box for later use.

[0056] 2. Preparation of polymer

[0057] Accurately transfer the polymer monomer HEMA and the cross-linking agent EDMA with a volume ratio of 99:1 into a 1.5mL doffer tube with a pipette gun, and add an equal volume of 2% DMPA isopropanol solution (w / v, mass-volume ratio ), and mix well with a vortexer. Accurately suck out 100 μL with a pipette gun, drop it on the surface of the aluminized polyester film, carefully press the silanized glass slide on the surface of the above solution, try to avoid the gener...

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Abstract

A method for quantitative analysis of ibuprofen in a biological sample by utilizing a cyclodextrin-modified holographic sensor is disclosed. The method includes main steps of subjecting the surface of a glass slide to silane modification, performing in-situ synthesis of hydroxyl ethyl methacrylate polymer on the surface, then loading the surface with gold nanoparticles, then radiating the glass slide with frequency-doubled Nd:YAG laser to obtain a reflective holographic sensor, modifying the gold nanoparticles with beta-cyclodextrin to obtain the beta-cyclodextrin-modified holographic sensor, putting the beta-cyclodextrin-modified holographic sensor into a series of ibuprofen standard solutions having different mass concentrations separately, recording reflection wavelengths, mapping a standard curve by adopting the reflection wavelengths as vertical coordinates and adopting the mass concentrations of the ibuprofen standard solutions as horizontal coordinates, collecting a reflection wavelength of a solution to be measured, substituting the reflection wavelength into the standard curve, and calculating the mass concentration of the ibuprofen in a sample to be detected. In the method, the holographic sensor is modified with the beta-cyclodextrin stably in a chemical bond manner, and the ibuprofen in the biological sample can be accurately and quantitatively analyzed. The method is simple and rapid.

Description

technical field [0001] The invention relates to a method for quantitatively analyzing ibuprofen in biological samples with a β-cyclodextrin modified holographic sensor, belonging to the technical field of drug analysis and detection. Background technique [0002] Drug analysis involves the qualitative and quantitative analysis of drugs in biological fluids such as plasma or urine, and is of great significance for obtaining and evaluating drug utilization, equivalence, and pharmacokinetic data. Ibuprofen (2-(4-isobutylphenyl)propionic acid) is a commonly used antipyretic and analgesic non-steroidal anti-inflammatory drug, which can relieve acute and chronic pain, rheumatism and muscle pain. Although it has significant medicinal effects, unreasonable or overdose may produce various adverse reactions, mainly manifested as gastrointestinal reactions, kidney damage, liver damage, cardiovascular events, etc. Moreover, it is an over-the-counter drug, which is cheap and easy to obt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 宋昕玥张家瑀王庆利
Owner LINYI UNIVERSITY
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