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Application of vaccarin in preparation of drug for preventing angiosteosis

A technology of flavonoid glycosides and anti-vascular calcification, which is applied in drug combinations, cardiovascular system diseases, and pharmaceutical formulations, can solve problems such as accelerating the healing speed of open wounds, and achieve the effect of expanding options and improving vascular calcification

Inactive Publication Date: 2017-11-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vaccarin mainly contains erythrine, triterpenoid saponins, flavonoid glycosides, lipids, cyclic peptides, fatty acids, and monosaccharides. Vaccarin (VAC) is the main active ingredient of vaccaria , the research in our laboratory found that glucuronoid glycosides can promote angiogenesis, resist endothelial cell damage caused by high glucose and oxidative stress, lower blood pressure and protect hypertensive nephropathy, improve lipid metabolism disorders in diabetes, and accelerate the healing of open wounds. Healing speed, suggesting that blancia glycosides may have cardiovascular protective effects, but there is no report on the role of blancie glycosides in the preparation of anti-vascular calcification drugs

Method used

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  • Application of vaccarin in preparation of drug for preventing angiosteosis
  • Application of vaccarin in preparation of drug for preventing angiosteosis
  • Application of vaccarin in preparation of drug for preventing angiosteosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] The replication of embodiment 1 vascular calcification model

[0011] The production method of vascular calcification model rats: referring to authoritative journals at home and abroad, we used 8-week-old male SD rats for intramuscular injection of vitamin D3. Weeks later, intramuscular injection of vitamin DB 300,000 units / kg was given again, and extensive vascular calcification appeared in the rats after 4 weeks.

Embodiment 2

[0012] Example 2 Experimental Grouping and Vaccina flavonoid glycoside (VAC) intervention

[0013] The 8-week-old SD rats were randomly divided into three groups:

[0014] (1) Normal control-normal saline group: as the normal control group, normal diet and drinking water were given all the time, and after 2 weeks, normal saline was subcutaneously infused for 2 weeks as a control of vaccinia flavonoid glycosides (VAC);

[0015] (2) Vascular calcification group-normal saline group was treated with vitamin D3 combined with nicotine to induce vascular calcification, and 2 weeks later, normal saline (saline) was injected intraperitoneally for 2 weeks as a control of flavonoid glycoside (VAC) of wangbuliuxing;

[0016] (3) Vascular calcification-flavone glycoside group (10mg / kg): Vitamin D3 combined with nicotine was used to induce vascular calcification, and 2 weeks later, vaccinia flavone glycoside (VAC, 10mg / kg) was injected intraperitoneally for 2 weeks.

Embodiment 3

[0017] Example 3 von Kossa staining and Alizarin red S staining

[0018] Referring to authoritative literature at home and abroad, rats were anesthetized with pentobarbital sodium, the chest cavity was opened, the heart was exposed, the ascending aorta was intubated through the left ventricle, the wall of the abdominal aorta was clipped, a small outlet was cut from the left atrial appendage, and 0.01M PBS was rapidly perfused. (37° C.) rinse until the effluent is clear, and then perfuse with pre-cooled (4° C.) 4% paraformaldehyde for fixation (first fast and then slow, slow down after the rat’s limbs are stretched). The thoracic aorta was taken out, fixed in 4% paraformaldehyde at 4°C for 24 hours, dehydrated, embedded in paraffin, sectioned, and subjected to routine vonKossa staining and Alizarin red S staining.

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Abstract

The invention relates to application of vaccarin in the preparation of a drug for preventing angiosteosis, belonging to the field of traditional Chinese medicine preparations. Vaccarin is applied to the development of the drug for preventing the angiosteosis so as to well treat angiosteosis. A cell experiment result of the application presents that vaccarin (VAC) is capable of remarkably decreasing deposition of calcium ions of vascular smooth muscle cells (VSMCs) caused by a calcification induction medium, inhibiting the increase of alkaline phosphatase (ALP) and simultaneously inhibiting the expression of a Runt-related transcription factor 2 (runt-related transcription factor 2, Runx2) of a calcification-related protein induced by the calcification induction medium and a bone morphogenetic protein 2 (Bone morphogenetic protein 2, BMP-2); and an in-vivo animal experiment further proves that VAC is capable of effectively treating a calcification model induced by vitamin D3 and nicotine and effectively improving the angiosteosis, is a traditional Chinese medicine for treating the angiosteosis and can be taken as novel drugs, health products and nutrition products for treating the angiosteosis, and a new path and measure are provided for treating angiosteosis-related drugs.

Description

technical field [0001] The invention relates to the development of anti-vascular calcification traditional Chinese medicine, and specifically designs the application of varicose flavonoid glycoside in the preparation of anti-vascular calcification medicine, which belongs to the field of traditional Chinese medicine development. Background technique [0002] Vascular calcification is a common vascular lesion, which is similar to the bone development process. Its pathological manifestations are mainly excessive deposition of calcium and phosphorus on the vessel wall, resulting in increased stiffness of the vessel wall and decreased compliance of the vessel, which in turn causes myocardial ischemia and hypoxia. Ventricular hypertrophy and heart failure, triggering thrombosis and plate rupture, and vascular calcification are the main factors of high mortality in cardiovascular and cerebrovascular diseases. Vascular calcification is regulated by many factors, including metabolism...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P9/14
CPCA61K31/7048
Inventor 邱丽颖孙海建朱雪雪龚蕾蕾蔡维维周跃涛
Owner JIANGNAN UNIV
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