Tissue culture method of edgeworthia chrysantha

A technology that is organized and fragrant, which is used in the field of plant tissue training, can solve problems such as reducing the quality and quality of seedlings, impossible to ensure stability, and increased risk of seedlings. The effect of sexual stability

Inactive Publication Date: 2018-03-13
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the literature uses mercuric chloride, which is extremely harmful to the environment, as a disinfectant, and the culture process has undergone a process of callus induction, which increases the risk of seedling variation caused by subculture proliferation during the callus differentiation process, and its traits cannot be guaranteed. stability, reducing the quality and quality of seedlings

Method used

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  • Tissue culture method of edgeworthia chrysantha
  • Tissue culture method of edgeworthia chrysantha
  • Tissue culture method of edgeworthia chrysantha

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Acquisition of aseptic materials and induction culture: cut the young and tender axillary buds of A. sinensis with good growth and no pests and diseases, wash them with tap water for 30 minutes, then shake them in an aqueous solution with a volume concentration of 1% detergent for 2 minutes, and use sterile Rinse with sterile water; soak 30s in 70% volume concentration ethanol water solution on the ultra-clean workbench, then soak in mixed disinfectant solution for 10min, and then rinse with sterile water for 3 times; blot dry water on sterilized filter paper, and dissect Peel off the 0.3mm axillary bud tip with a knife, then inoculate the axillary bud tip into a culture bottle filled with cluster bud induction medium, cover the bottle cap and seal the bottle mouth with a parafilm, and place it in an incubator at 24°C with a light of 1500lx Cultivate under conditions for 15 days; the mixed disinfectant is a mixture of Tween 20 and 84 disinfectant with a volume ratio ...

Embodiment 2

[0038] The influence of the different disinfectants of embodiment 2 on the growth of Agarica explants

[0039] 10% hydrogen peroxide (H 2 o 2 ) aqueous solution, mercuric chloride (HgCl) with a mass concentration of 0.1% 2 ) aqueous solution and the mixed disinfectant solution in embodiment 1 to sterilize the explant material (i.e. axillary buds) of A. sinensis, and other operations are the same as in embodiment 1. The materials were respectively inoculated into culture bottles filled with cluster bud induction medium, and the bottles were sealed with a parafilm and then placed in an incubator. After culturing for 15 days at 24° C. under the condition of light of 1500 lx, observe and count the effects of different disinfectants on the induction and growth of Azalea buds. The results are shown in Table 1. The results showed that the mixed disinfectant and mass concentration of 0.1% HgCl 2 The disinfection effect is basically the same, the disinfection effect of hydrogen per...

Embodiment 3

[0042] Example 3 Different Hormone Combinations Effect on the Induction of Aroma Buds

[0043] The axillary buds of A. japonica were respectively inoculated in the MS basal medium (i.e. cluster bud induction medium) added with different concentrations of IAA, 6-BA and NAA. Other operations were the same as in Example 1. The growth results of adventitious buds of A. japonica are shown in Table 2. Show. The results showed that the medium with different combinations of hormones could induce the formation of shoots to varying degrees. Among them, the medium MS+IAA0.3mg / L+6-BA1.0mg / L+NAA0.2mg / L+50mg / LPVP is the best, the most regenerated buds are induced, the growth is fast, the average bud length is 3.05cm, the buds The induction rate reached 95%, the seedlings were large and strong, and there was no phenomenon of vitrified seedlings, and the incisions of the culture medium without adding PVP showed different degrees of browning.

[0044] Bud induction rate = number of bud induc...

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Abstract

The invention discloses a tissue culture method of edgeworthia chrysantha, so that a large number of high-quality edgeworthia chrysantha seedlings can be obtained in a short time, so as to meet the market requirements. The method comprises the following steps: obtaining of an asepsis material, induced culture of buds, multiplication of the buds and rooting. Axillary buds are taken as explants; thetissue culture method is applied for rapid propagation; nutritional factors such as yeast extract are added into a culture medium, so that the defects of being long in cycle, low in propagation coefficient, easy in infection and spreading of viruses and the like in conventional cutting and division propagation of the edgeworthia chrysantha are overcome; and tissue culture seedlings produced by the method have the advantages of being less in viruses, stable in heredity and the like.

Description

(1) Technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid seedling propagation of A. japonicus. By this method, large-scale production of fine seedlings can be carried out. (2) Background technology [0002] Edgeworthia chrysantha (Edgeworthia chrysantha) is a plant of the genus Thymus. In my country, it is mainly distributed in the south of the Yangtze River Basin in my country. Its leaves are dark green, its flowers are beautiful, and its flowering period is long. It is a very beautiful garden flower. In addition to its ornamental value, it also has important medical and industrial uses. The stem bark fiber can be used as raw material for high-grade paper and artificial cotton. The whole plant can be used as medicine to relax tendons and activate collaterals, reduce inflammation and relieve pain, and can treat bruises and rheumatism; it can also be used as veterinary medicine to...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 邹克琴管峰洪婷婷
Owner CHINA JILIANG UNIV
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