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Serum replacement for cell culture

A technology of serum substitutes and cell culture, applied in the field of serum substitutes for medium, to achieve the effects of reducing production costs, prolonging cell culture, and reducing costs

Inactive Publication Date: 2018-05-04
成都源泉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is that the medium containing serum or not containing serum in the prior art will have a certain impact on the culture and growth of cells, and the purpose is to provide a serum substitute for cell culture, which can be added to the commonly used basal medium Cells can be cultured in the environment, and can be used as a substitute for serum; it does not contain IgG, which greatly simplifies the antibody purification process of monoclonal antibody production; cell culture is significantly prolonged without cells dying; it is conducive to the production of proteins and antibodies; the cost can be greatly reduced, and it can be used reduce manufacturing cost

Method used

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  • Serum replacement for cell culture
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Embodiment 1

[0017] A serum substitute for cell culture, comprising raw materials with the following mass concentrations: manganese dichloride 50mg / L, sodium selenite 40mg / L, ammonium molybdate 45mg / L, iron citrate 50mg / L, choline tartrate 3mg / L, vitamin D2 6mg / L, vitamin K2 3mg / L, vitamin K3 4mg / L, adenine 3mg / L, guanine hydrochloride 5mg / L, uracil 6mg / L, linoleic acid 5mg / / L, Cholesterol 4mg / L, Insulin 0.005mg / L, Hydrocortisone 0.005mg / L, Dexamethasone 0.2mg / L, Yeast Hydrolyzate 500mg / L, Milk Protein Hydrolyzate 400mg / L, Casein Hydrolyzate 600mg / L , Malt peptone 500mg / L.

Embodiment 2

[0019] A serum substitute for cell culture, comprising raw materials with the following mass concentrations: manganese dichloride 20mg / L, sodium selenite 30mg / L, ammonium molybdate 40mg / L, iron citrate 20mg / L, choline tartrate 2mg / L, vitamin D2 0.3mg / L, vitamin K2 0.8mg / L, vitamin K32mg / L, adenine 8mg / L, guanine hydrochloride 2mg / L, uracil 3mg / L, linoleic acid 2mg / L, Cholesterol 4mg / L, Insulin 0.2mg / L, Hydrocortisone 0.00001mg / L, Dexamethasone 0.01mg / L, Yeast Hydrolyzate 10mg / L, Milk Protein Hydrolyzate 30mg / L, Casein Hydrolyzate 30mg / L , Malt peptone 20mg / L.

Embodiment 3

[0021] A serum substitute for cell culture, comprising raw materials with the following mass concentrations: manganese dichloride 100mg / L, sodium selenite 90mg / L, ammonium molybdate 80mg / L, iron citrate 100mg / L, choline tartrate 5mg / L, vitamin D2 8mg / L, vitamin K2 9mg / L, vitamin K3 5mg / L, adenine 4mg / L, guanine hydrochloride 8mg / L, uracil 6mg / L, linoleic acid 8mg / L, cholesterol 7mg / L, insulin 0.8mg / L, hydrocortisone 0.001mg / L, dexamethasone 0.6mg / L, yeast hydrolyzate 600mg / L, milk protein hydrolyzate 700mg / L, casein hydrolyzate 850mg / L, Malt peptone 300mg / L.

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Abstract

The invention discloses a serum replacement for cell culture. The serum replacement is prepared from the following raw materials: manganese chloride, sodium selenite, ammonium molybdate, ferric citrate, choline bitartrate, vitamin D2, vitamin K2, vitamin K3, adenine, guanine hydrochloride, uracil, linoleic acid, cholesterol, insulin, hydrocortisone, dexamethasone, yeast hydrolysate, lactoprotein hydrolysate, casein hydrolysate and malt peptone. The serum replacement disclosed by the invention is added into a common basic culture medium and can be used for culturing cells, and can completely replace serum; the serum replacement does not contain IgG so that a monoclonal antibody produced antibody purification technology is greatly simplified; the cell culture is remarkably prolonged and thecells do not die; the production of protein and antibodies is facilitated; the cost can be greatly reduced and the production cost can be reduced.

Description

technical field [0001] The present invention relates to a serum substitute for culture medium, in particular to a serum substitute for cell culture. Background technique [0002] After decades of development, cell culture is now widely used in biology, medical technology, pharmaceuticals and other fields, forming a huge industry. Generally, bovine serum is often added to animal cell culture. Usually, animal cell culture is used for protein, antibody and vaccine production without using serum. 2) The quality difference between batches of products is reduced, and the repeatability and accuracy of the experiment are improved; 3) The composition of serum-free medium is clear, which avoids the influence of components on experimental research and is conducive to the development of cell metabolism. Basic research and the effect of specific components on cells; ④The serum-free medium is completely made of synthetic compounds, so the composition is determined, and the interference o...

Claims

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Application Information

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IPC IPC(8): C12N5/00
CPCC12N5/0018C12N2500/10C12N2500/30C12N2500/38C12N2500/40C12N2500/74C12N2501/33C12N2501/39C12N2501/998
Inventor 刘才明
Owner 成都源泉生物科技有限公司
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