Decoloring agent containing Pleurotus citrinopileatus residue extract
A technology of yellow elm mushroom and decolorizing agent, which is applied in the field of reuse of yellow mushroom dregs, can solve the problems of hidden safety hazards in large-scale production areas of edible fungi, low comprehensive utilization rate of dregs, breeding of miscellaneous bacteria and pathogenic bacteria, etc., and achieves The effect of easy to obtain raw materials, low cost and high laccase activity
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Embodiment 1
[0032] The present embodiment relates to a kind of preparation method of decolorizing agent, comprises the steps:
[0033] 1) Slowly mix the fungus slag of yellow mushroom and citric acid with a pH of 4.8 according to the mass volume ratio of 1:4, and then shake it in a shaker at 190rpm for 4 hours at 28°C to extract the fungus residue of yellow mushroom , the lower part of the sediment obtained after centrifugation is the residue of bacteria residue;
[0034] The dregs of the yellow elm mushrooms are cultured for 22 to 25 days after the mushrooms are harvested; the yellow mushrooms are cultured on the following medium, including 58 parts of kenaf stalks in parts by weight , 20 parts of sawdust, 20 parts of bran, 1 part of gypsum, 1 part of lime;
[0035] 2) add distilled water to it after drying the residue of the bacteria residue until its water content is 50-65%, inoculate Pleurotus ostreatus strains in it, and cultivate it statically for 21 days under the condition of 28°...
Embodiment 2
[0037] Compared with Example 1, the difference is that the Pleurotus ostreatus is fermented and cultured for 19d in the step 2).
experiment example
[0047] The relevant properties of Examples 1-4 and Comparative Examples 1-4 were tested.
[0048] Determination of Enzyme Activity:
[0049] Experiment method: laccase activity determination method: accurately pipette 10ml of 100mmol / L malonate-sodium malonate buffer solution and 0.6mmol / L ABTS solution, mix, shake well, and bathe in 30℃ water for 5min; take the mixture 200ul in the well of the microplate plate, set to zero at 420nm; accurately add 10ul of the decolorizing agent in the embodiment and the comparative example, record the absorbance value immediately, record once every 1min, a total of 3 times, take the average value, and convert it into Change in absorbance (OD 420 Variety).
[0050] Indigo decolorization experiment: Take 3 parts of 10ml 100mg / L indigo solution, add 2ml 2.5g / L HBT solution, and 20ml decolorizing agent of Example and Comparative Example, place in 30°C, 70r / min water bath shaker, every Absorbance was measured every 2 hours.
[0051] Congo red ...
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