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Preparation method and application of Pueraria lobata extract and/or pueraria flavones

A technology of kudzu root extract and kudzu root flavonoids, applied in the field of preparation of kudzu root extract and/or kudzu root flavonoids, to achieve the effects of reducing BOD and COD, improving curative effect, and increasing yield

Inactive Publication Date: 2018-05-11
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aiming at the existing technical defects, the present invention develops a new technology of using pueraria starch mother liquor as raw material, first extracting pueraria extract, and then extracting pueraria flavonoids, which has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of Pueraria Radix Extract and Pueraria Radix Flavonoids

[0041] After 100 kilograms of kudzu root are washed, pulverized and crossed 80 orders, kudzu root slurry is obtained; the kudzu root slurry is deslagged, desilted, and kudzu root starch is separated to obtain kudzu root starch and kudzu root starch mother liquor; add 0.01% (weight / weight) to kudzu root starch mother liquor Calcium sulfate, stir evenly; heat up to 100°C, keep warm for 0 minutes; cool down to 0°C for 24 hours, filter to obtain Pueraria root extract and supernatant; dry and pulverize Pueraria root extract to obtain Pueraria root extract product. Put the macroporous resin and supernatant on the column according to the volume ratio of macroporous resin: supernatant 1:1 and the speed of 5% (column volume / minute); after the column is completed, wash with 20 times the volume of the resin The resin is then eluted with 100% ethanol of 20 times the volume of the resin, and the ethanol ...

Embodiment 2

[0046] Example 2 Preparation of Pueraria Radix Extract and Pueraria Radix Flavonoids

[0047] After 100 kilograms of kudzu root are washed, pulverized and crossed 80 orders, kudzu root pulp is obtained; kudzu root pulp is deslagging, desilted, and kudzu root starch is separated to obtain kudzu root starch and kudzu root starch mother liquor; add 5% (weight / weight) to kudzu root starch mother liquor magnesium sulfate, stirred evenly; heated to 50°C, and kept warm for 60 minutes; stood still for 0.1 hour to 40°C, filtered to obtain the Pueraria root extract and supernatant; dried the Pueraria root extract, and pulverized to obtain the Pueraria root extract product. Put the macroporous resin and the supernatant on the column according to the volume ratio macroporous resin: supernatant 1:1000 and the speed of 0.01% (times resin volume / minute); after the column is completed, use 0.1 times the resin volume of water The resin is washed, and then eluted with 10% ethanol of 0.1 times t...

Embodiment 3

[0052] Example 3 The preparation of kudzu root extract and kudzu root flavonoids

[0053] After 100 kilograms of kudzu root are cleaned, pulverized and crossed 80 orders, the kudzu root slurry is obtained; the kudzu root slurry is deslagging, desanded, and the kudzu root starch is separated to obtain the kudzu root starch and kudzu root starch mother liquor; add 1% (weight / weight) of kudzu root starch to the kudzu root starch mother liquor aluminum sulfate, stir evenly; heat up to 60°C, keep warm for 10 minutes; cool down to 40°C for 6 hours, filter to obtain Pueraria root extract and supernatant; dry Pueraria root extract, pulverize to obtain Pueraria root extract product. Put the macroporous resin and the supernatant on the column according to the volume ratio macroporous resin:supernatant 1:100 and the speed of 0.1% (column volume / minute); after the column is completed, wash with 1 times the volume of the resin The resin is then eluted with 40% ethanol of 1 times the volume...

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PUM

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Abstract

The invention belongs to the field of food or medicines, in particular relates to a preparation method and application of pueraria lobata extract and / or pueraria flavones. The preparation method of Pueraria lobata extract and / or pueraria flavones provided by the present invention can increase the precipitation rate of proteins and other colloidal components to 95% or more, not only improves the yield and curative effect of the Pueraria lobata extract, but also facilitates the use of macroporous resin in the next step for extraction of the Pueraria flavones, the service life of the macroporousresin can be extended to100 cycles or more, the recovery rate of the pueraria flavones can be as high as 95%, and the purity can be increased to 80%. The pueraria extract and / or pueraria flavones prepared by the method can be used as a substitute for Pueraria lobata, and is used for production and formulation of foods, health foods and pharmaceuticals related to anti-oxidation, blood vessel-dilation, hypoglycemic, lipid-lowering, antihypertensive, antibacterial alcohol-relieving and other related functions. The pueraria extract and the pueraria flavones have important economic value, and through the method, the BOD and COD of the pueraria lobata wastewater can be greatly reduced, and the method has important environmental significance.

Description

technical field [0001] The invention belongs to the field of food or medicine, and in particular relates to a preparation method and application of kudzu root extract and / or kudzu root flavonoids. Background technique [0002] Pueraria lobata, the dried root of the leguminous plant Pueraria lobata, sweet and pungent in taste, calm in nature, and returns to the spleen, stomach, lung and bladder meridians; it has the functions of relieving muscles and reducing fever, clearing rashes, promoting body fluid and quenching thirst, promoting yang and relieving diarrhea; commonly used Symptoms include fever, stiff neck and back pain, opaque measles, pyretic disease and thirst, yin deficiency and thirst, heat diarrhea and dysentery, spleen deficiency and diarrhea. In addition to Pueraria starch, Pueraria also contains a variety of active ingredients, such as Pueraria flavonoids, whose representative components are Puerarin, etc. Pueraria has a wide range of pharmacological effects, su...

Claims

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Application Information

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IPC IPC(8): A61K36/488A61P39/06A61P9/08A61P3/10A61P3/06A61P9/12A61P25/28A61P39/02A23L33/105
CPCA23V2002/00A61K36/488A61K2236/53A61K2236/55A23V2200/30A23V2200/326A23V2200/328A23V2200/3262A23V2200/322A23V2200/334A23V2250/21A23V2250/2116
Inventor 叶小利李学刚李滟临李亚松
Owner SOUTHWEST UNIVERSITY
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