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Endophytic bacterium of halophyte plants and application of endophytic bacterium

A technology of endophytic bacteria and halophytes, applied in the field of microorganisms, can solve the problems of unseen patent research and application reports, few data reports, etc., and achieve the effect of promoting healthy growth of plants, increasing biomass, and increasing biomass

Active Publication Date: 2018-05-11
BINZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are few reports at home and abroad on the promotion of plant growth in salinized petroleum-contaminated soil by endophytic bacteria in halophytes and the improvement of the plant-microbe system’s ability to degrade petroleum pollutants, and there are no relevant patent research and application reports.

Method used

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  • Endophytic bacterium of halophyte plants and application of endophytic bacterium
  • Endophytic bacterium of halophyte plants and application of endophytic bacterium
  • Endophytic bacterium of halophyte plants and application of endophytic bacterium

Examples

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Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Isolation of Reed Endophytic Bacteria Degrading Petroleum Hydrocarbons

[0047] The wild plants of Phragmites reed growing healthily in oil-polluted heavily saline-alkali areas were collected, and the endophytic bacteria isolation and screening test was carried out immediately.

[0048] The whole plant was washed with tap water for 30 minutes, and then washed with distilled water for 3 times, each time for 3 minutes. Drain the water on the surface of the plant, divide the plant into three parts: root, stem and leaf, soak each part in 70% ethanol solution for 2 minutes, wash with sterile water for 3 times, then put it into 1.2% sodium hypochlorite solution for shaking for 30 minutes, rinse with sterile water for 3 minutes times, 2 minutes each time. Take 100 μL of sterile water for the last rinse, spread it on LB solid medium (yeast powder 5.0 g / L, tryptone 10.0 g / L, NaCl 10.0 g / L, agar 15.0 g / L, pH 7.4) and incubate for 24 h, Verify complete surface sterili...

Embodiment 2

[0049] Example 2: Screening, identification and preservation of reed endogenous growth-promoting bacteria that degrade petroleum hydrocarbons

[0050]Endogenous growth-promoting bacteria that efficiently degrade petroleum hydrocarbons were screened by measuring the ACC deaminase-producing activity, siderophore-producing ability, IAA-producing ability, phosphorus-dissolving ability and the ability to degrade total petroleum hydrocarbons in diesel oil obtained from the strains isolated above.

[0051] 1. Determination of ACC deaminase activity

[0052] 1-aminocyclopropane-1-carboxylic acid (ACC) in plants is a precursor for ethylene synthesis. Endophytic bacteria producing ACC deaminase can use this enzyme to degrade ACC into α- Butyruvate and ammonia reduce the concentration of ethylene under adverse conditions, reduce the production of harmful ethylene, and promote the rapid growth of plants.

[0053] Refer to Penrose D M and Glick B R [Document 15: Penrose D M, Glick B R. Me...

Embodiment 3

[0083] Embodiment 3: Effect of Pseudomonas aeruginosa L10 (Pseudomonas aeruginosa L10) degrading petroleum hydrocarbons under different NaCl concentrations

[0084] The pure hydrocarbons tested were three normal alkanes, n-undecane, n-hexadecane, and n-octadecane, and three polycyclic aromatic hydrocarbons, naphthalene, phenanthrene, and pyrene.

[0085] The number of prepared cells is about 1×10 8 The bacterial suspension of CFU / mL (referring to the method of embodiment 2), inserts the inorganic salt liquid culture medium (the content of single hydrocarbon substance is 200mg / L) with single hydrocarbon substance as sole carbon source by 5% inoculum size. Wherein, the NaCl concentration in the culture medium was respectively set to 2%, 4%, and 6% according to the strain tolerance. The culture medium without adding bacteria and adding a single hydrocarbon was used as the blank control, and three replicates were set up. The pH value was adjusted to 7.4, and after culturing on a...

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Abstract

The invention belongs to the technical field of microorganisms and particularly relates to an endophytic bacterium of halophyte plants and an application of the endophytic bacterium. The endophytic bacterium of the halophyte plants is completely named Pseudomonas aeruginosa L10, and the strain is preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number being CGMCC No.9406 on July, 3rd, 2014. The Pseudomonas aeruginosa L10 can be colonized in phragmites australis, and can promote healthy growth of plants in petroleum-contaminated salinity soil, thereby remarkably increasing biomass of host plants and cooperating with the host plants to promote degradation of petroleum hydrocarbon in soil.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to endophytic bacteria of halophytes and applications thereof. Background technique [0002] Soil oil pollution poses a serious threat to human health and ecosystem security, and is an important environmental problem that needs to be solved urgently. [0003] In my country, most of the oil fields are located in areas with salinized soil, and oil enters the soil during the process of exploitation, storage, transportation, and processing, which easily forms oil-polluted salinized soil. A large amount of saline drilling waste entering the soil can also easily cause salinization of oil-contaminated soil around the well site [Document 1: Qin X, Tang J C, Li D S, et al. Effect of salinity on the bioremediation of petroleum hydrocarbons in a saline-alkaline soil[J]. Letters in applied microbiology, 2012,55(3):210-217]. Studies have proved that soil salinization signifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/385
CPCB09C1/10C12N1/205C12R2001/385
Inventor 吴涛许杰谢文军姚志刚李甲亮王怡杨红军孙春龙
Owner BINZHOU UNIV
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