Method for preparing ACE (angiotensin converting enzyme) inhibitory peptide by using shellfish meat

A technology for inhibiting peptides and shellfish meat, applied in the field of preparing ACE-inhibiting peptides from shellfish meat, can solve the problems of high yield of ACE-inhibiting peptides, immature technology, and large amount of enzymes, so as to reduce the difficulty of operation, improve the fermentation efficiency, and improve the efficiency of the fermentation process. separation effect

Active Publication Date: 2018-05-18
欧美生物技术孵化转移中心香港有限公司
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, enzymatic extraction of ACE inhibitory peptides from shellfish is relatively mature, but the amount of enzyme used is large and the cost is high; the cost of microbial fermentation is low, and the yield of ACE inhibitory peptides is high, but the technology is immature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing ACE (angiotensin converting enzyme) inhibitory peptide by using shellfish meat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A. Fresh shellfish is steamed, chopped, and placed in a -20°C refrigerator for freezing. After 6 hours, it is transferred to a vacuum freeze dryer, freeze-dried for 48 hours, and crushed through a 40-mesh sieve to obtain dried shellfish powder;

[0043] B. adding the dried shellfish powder to distilled water for homogenization according to the material-to-liquid ratio of 1:3, adjusting the pH to 6.3, then adding 40 mg / ml magnetic carbon microspheres, and autoclaving for 20 minutes to obtain the culture medium;

[0044] The preparation method of described magnetic carbon microsphere is as follows:

[0045] (1) Dissolve waste bean dregs and potassium carbonate in distilled water at a mass ratio of 1:2, immerse at room temperature for 4 hours, then dry to a water content of 2%, and transfer to a tube furnace, under the protection of 400ml / min nitrogen, at 6 Heating at the rate of ℃ / min to 600 ℃, keeping it warm for 75 minutes, washing the sample until neutral after cooling...

Embodiment 2

[0059] A. Fresh shellfish is steamed, chopped, and placed in a -20°C refrigerator for freezing. After 6 hours, it is transferred to a vacuum freeze dryer, freeze-dried for 48 hours, and crushed through a 40-mesh sieve to obtain dried shellfish powder;

[0060] B. Add distilled water to the dry shellfish meat powder and carry out homogenization according to the ratio of material to liquid 1: 4, adjust the pH to be 7.0, then add 60mg / ml magnetic carbon microspheres, after autoclaving for 30min, obtain the culture medium;

[0061] The preparation method of described magnetic carbon microsphere is as follows:

[0062] (1) Dissolve waste bean dregs and potassium carbonate in distilled water at a mass ratio of 1:2, immerse at room temperature for 4 hours, then dry to a water content of 5%, and transfer to a tube furnace. Heat at a rate of ℃ / min to 650 ℃, keep warm for 100min, wash the sample until neutral after cooling, dry and grind into powder, and pass through a 100-mesh sieve to...

Embodiment 3

[0076] A. Fresh shellfish is steamed, chopped, and placed in a -20°C refrigerator for freezing. After 6 hours, it is transferred to a vacuum freeze dryer, freeze-dried for 48 hours, and crushed through a 40-mesh sieve to obtain dried shellfish powder;

[0077] B. adding distilled water to the dried shellfish meat powder for homogenization according to the ratio of material to liquid 4, adjusting the pH to 6.5, then adding 50 mg / ml magnetic carbon microspheres, and autoclaving for 30 minutes to obtain the culture medium;

[0078] The preparation method of described magnetic carbon microsphere is as follows:

[0079] (1) Dissolve waste bean dregs and potassium carbonate in distilled water at a mass ratio of 1:2, immerse at room temperature for 4 hours, then dry to a water content of 4%, and transfer to a tube furnace. Heat at a rate of ℃ / min to 600 ℃, keep it warm for 80 minutes, wash the sample until neutral after cooling, dry and grind it into powder, pass through a 80-mesh si...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for preparing ACE (angiotensin converting enzyme) inhibitory peptide by using shellfish meat. The method has the advantages that the utilization rate and extraction rate of enzyme are improved, the activity of the ACE inhibitory peptide is high, and the good blood pressure reducing effect is realized. The method comprises the following steps of fermenting the shellfish meat by bacillus subtilis and aspergillus niger, and assisting by magnetic carbon microspheres, so as to improve the activity and purity of the ACE inhibitory peptide, and improve the fermenting rate; finally, purifying the ACE inhibitory peptide by a magnetic silicon dioxide adsorption column, so as to obtain the product. The prepared ACE inhibitory peptide has the advantages that the activity is high, the inhibition rate is high, and the good blood pressure reducing function is realized.

Description

technical field [0001] The invention belongs to the technical field of natural substance extraction, and in particular relates to a method for preparing ACE inhibitory peptides from shellfish meat. Background technique [0002] Hypertension refers to an increase in systemic arterial blood pressure (systolic and / or diastolic) as the main feature (systolic blood pressure ≥ 140 mm Hg, diastolic blood pressure ≥ 90 mm Hg), which may be accompanied by heart, brain, kidney, etc. Clinical syndrome of functional or organic damage to organs. At present, although the antihypertensive drugs commonly used in clinic have a good antihypertensive effect, they also have some toxic side effects and adverse reactions. [0003] From the perspective of the pathogenesis of hypertension, the inactive angiotensin I is converted into angiotensin II with strong vasoconstriction activity under the catalysis of angiotensin I-converting enzyme (ACE), which degrades the vasodilation hormone. Peptides ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P21/00C12P21/06C12R1/125C12R1/685
CPCC12P21/00C12P21/06C12P39/00
Inventor 周文辽
Owner 欧美生物技术孵化转移中心香港有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap